DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects

The objective of the present work was to study the fine kinetics of DNA repair in xeroderma pigmentosum (XP) syndrome, a complex disorder linked to a deficiency in repair that increases cancer susceptibility. The repair process was evaluated by the comet assay (CA) in cells from 2 XP patients and 9...

Descripción completa

Guardado en:
Detalles Bibliográficos
Publicado: 2014
Materias:
DNA
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07318898_v33_n4_p279_PrietoGonzalez
http://hdl.handle.net/20.500.12110/paper_07318898_v33_n4_p279_PrietoGonzalez
Aporte de:
id paper:paper_07318898_v33_n4_p279_PrietoGonzalez
record_format dspace
spelling paper:paper_07318898_v33_n4_p279_PrietoGonzalez2023-06-08T15:43:54Z DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects Base excision repair (BER) Comet assay DNA repair mechanisms Hydrogen peroxide Nucleotide excision repair (NER) rUVA/B Xeroderma pigmentosum (XP) base DNA hydrogen peroxide sunscreen hydrogen peroxide adult Article blood sampling cancer susceptibility case report cell migration child clinical feature comet assay controlled study DNA repair excision repair female human human cell kinetics leukocyte lotion male nutritional counseling oxidation oxidative stress radiation exposure room temperature school child skin cancer skin defect sun exposure ultraviolet A radiation ultraviolet B radiation xeroderma pigmentosum chemically induced DNA damage drug effects genetics leukocyte metabolism pathology radiation response time toxicity ultraviolet radiation xeroderma pigmentosum Adult Child Comet Assay DNA Damage DNA Repair Female Humans Hydrogen Peroxide Kinetics Leukocytes Male Time Factors Ultraviolet Rays Xeroderma Pigmentosum The objective of the present work was to study the fine kinetics of DNA repair in xeroderma pigmentosum (XP) syndrome, a complex disorder linked to a deficiency in repair that increases cancer susceptibility. The repair process was evaluated by the comet assay (CA) in cells from 2 XP patients and 9 controls exposed to UVA/B (UVA 366/UVB 280 nm) and H2O2(150 μM) at temperatures of 4, 15, and 37°C. Samples were taken at 2-min intervals during the first 10 min to analyze the “fine kinetics” repair during the initial phase of the curve, and then at 15, 20, 25, 30, 45, 60, and 120 min. CA evaluation of DNA repair activity points to BER/NER initiation in the first 30 min with both inductors at 37°C and 15°C, but final comet length showed differences according to treatment. Repair kinetics during 120 min showed a good correlation with clinical features in both XP patients. Differences in final comet length were less pronounced in XP cells treated with H2O2than with UVA/B, probably because the peroxide produces mainly base oxidation but less bulky lesions; UVA/B generates a mixture of both. These findings reinforce the value of CA in testing in DNA repair ability or exposure monitoring. © 2014 Begell House, Inc. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07318898_v33_n4_p279_PrietoGonzalez http://hdl.handle.net/20.500.12110/paper_07318898_v33_n4_p279_PrietoGonzalez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Base excision repair (BER)
Comet assay
DNA repair mechanisms
Hydrogen peroxide
Nucleotide excision repair (NER)
rUVA/B
Xeroderma pigmentosum (XP)
base
DNA
hydrogen peroxide
sunscreen
hydrogen peroxide
adult
Article
blood sampling
cancer susceptibility
case report
cell migration
child
clinical feature
comet assay
controlled study
DNA repair
excision repair
female
human
human cell
kinetics
leukocyte
lotion
male
nutritional counseling
oxidation
oxidative stress
radiation exposure
room temperature
school child
skin cancer
skin defect
sun exposure
ultraviolet A radiation
ultraviolet B radiation
xeroderma pigmentosum
chemically induced
DNA damage
drug effects
genetics
leukocyte
metabolism
pathology
radiation response
time
toxicity
ultraviolet radiation
xeroderma pigmentosum
Adult
Child
Comet Assay
DNA Damage
DNA Repair
Female
Humans
Hydrogen Peroxide
Kinetics
Leukocytes
Male
Time Factors
Ultraviolet Rays
Xeroderma Pigmentosum
spellingShingle Base excision repair (BER)
Comet assay
DNA repair mechanisms
Hydrogen peroxide
Nucleotide excision repair (NER)
rUVA/B
Xeroderma pigmentosum (XP)
base
DNA
hydrogen peroxide
sunscreen
hydrogen peroxide
adult
Article
blood sampling
cancer susceptibility
case report
cell migration
child
clinical feature
comet assay
controlled study
DNA repair
excision repair
female
human
human cell
kinetics
leukocyte
lotion
male
nutritional counseling
oxidation
oxidative stress
radiation exposure
room temperature
school child
skin cancer
skin defect
sun exposure
ultraviolet A radiation
ultraviolet B radiation
xeroderma pigmentosum
chemically induced
DNA damage
drug effects
genetics
leukocyte
metabolism
pathology
radiation response
time
toxicity
ultraviolet radiation
xeroderma pigmentosum
Adult
Child
Comet Assay
DNA Damage
DNA Repair
Female
Humans
Hydrogen Peroxide
Kinetics
Leukocytes
Male
Time Factors
Ultraviolet Rays
Xeroderma Pigmentosum
DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects
topic_facet Base excision repair (BER)
Comet assay
DNA repair mechanisms
Hydrogen peroxide
Nucleotide excision repair (NER)
rUVA/B
Xeroderma pigmentosum (XP)
base
DNA
hydrogen peroxide
sunscreen
hydrogen peroxide
adult
Article
blood sampling
cancer susceptibility
case report
cell migration
child
clinical feature
comet assay
controlled study
DNA repair
excision repair
female
human
human cell
kinetics
leukocyte
lotion
male
nutritional counseling
oxidation
oxidative stress
radiation exposure
room temperature
school child
skin cancer
skin defect
sun exposure
ultraviolet A radiation
ultraviolet B radiation
xeroderma pigmentosum
chemically induced
DNA damage
drug effects
genetics
leukocyte
metabolism
pathology
radiation response
time
toxicity
ultraviolet radiation
xeroderma pigmentosum
Adult
Child
Comet Assay
DNA Damage
DNA Repair
Female
Humans
Hydrogen Peroxide
Kinetics
Leukocytes
Male
Time Factors
Ultraviolet Rays
Xeroderma Pigmentosum
description The objective of the present work was to study the fine kinetics of DNA repair in xeroderma pigmentosum (XP) syndrome, a complex disorder linked to a deficiency in repair that increases cancer susceptibility. The repair process was evaluated by the comet assay (CA) in cells from 2 XP patients and 9 controls exposed to UVA/B (UVA 366/UVB 280 nm) and H2O2(150 μM) at temperatures of 4, 15, and 37°C. Samples were taken at 2-min intervals during the first 10 min to analyze the “fine kinetics” repair during the initial phase of the curve, and then at 15, 20, 25, 30, 45, 60, and 120 min. CA evaluation of DNA repair activity points to BER/NER initiation in the first 30 min with both inductors at 37°C and 15°C, but final comet length showed differences according to treatment. Repair kinetics during 120 min showed a good correlation with clinical features in both XP patients. Differences in final comet length were less pronounced in XP cells treated with H2O2than with UVA/B, probably because the peroxide produces mainly base oxidation but less bulky lesions; UVA/B generates a mixture of both. These findings reinforce the value of CA in testing in DNA repair ability or exposure monitoring. © 2014 Begell House, Inc.
title DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects
title_short DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects
title_full DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects
title_fullStr DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects
title_full_unstemmed DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects
title_sort dna repair kinetic of hydrogen peroxide and uva/b induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects
publishDate 2014
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07318898_v33_n4_p279_PrietoGonzalez
http://hdl.handle.net/20.500.12110/paper_07318898_v33_n4_p279_PrietoGonzalez
_version_ 1768544645380308992