DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects
The objective of the present work was to study the fine kinetics of DNA repair in xeroderma pigmentosum (XP) syndrome, a complex disorder linked to a deficiency in repair that increases cancer susceptibility. The repair process was evaluated by the comet assay (CA) in cells from 2 XP patients and 9...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07318898_v33_n4_p279_PrietoGonzalez http://hdl.handle.net/20.500.12110/paper_07318898_v33_n4_p279_PrietoGonzalez |
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paper:paper_07318898_v33_n4_p279_PrietoGonzalez2023-06-08T15:43:54Z DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects Base excision repair (BER) Comet assay DNA repair mechanisms Hydrogen peroxide Nucleotide excision repair (NER) rUVA/B Xeroderma pigmentosum (XP) base DNA hydrogen peroxide sunscreen hydrogen peroxide adult Article blood sampling cancer susceptibility case report cell migration child clinical feature comet assay controlled study DNA repair excision repair female human human cell kinetics leukocyte lotion male nutritional counseling oxidation oxidative stress radiation exposure room temperature school child skin cancer skin defect sun exposure ultraviolet A radiation ultraviolet B radiation xeroderma pigmentosum chemically induced DNA damage drug effects genetics leukocyte metabolism pathology radiation response time toxicity ultraviolet radiation xeroderma pigmentosum Adult Child Comet Assay DNA Damage DNA Repair Female Humans Hydrogen Peroxide Kinetics Leukocytes Male Time Factors Ultraviolet Rays Xeroderma Pigmentosum The objective of the present work was to study the fine kinetics of DNA repair in xeroderma pigmentosum (XP) syndrome, a complex disorder linked to a deficiency in repair that increases cancer susceptibility. The repair process was evaluated by the comet assay (CA) in cells from 2 XP patients and 9 controls exposed to UVA/B (UVA 366/UVB 280 nm) and H2O2(150 μM) at temperatures of 4, 15, and 37°C. Samples were taken at 2-min intervals during the first 10 min to analyze the “fine kinetics” repair during the initial phase of the curve, and then at 15, 20, 25, 30, 45, 60, and 120 min. CA evaluation of DNA repair activity points to BER/NER initiation in the first 30 min with both inductors at 37°C and 15°C, but final comet length showed differences according to treatment. Repair kinetics during 120 min showed a good correlation with clinical features in both XP patients. Differences in final comet length were less pronounced in XP cells treated with H2O2than with UVA/B, probably because the peroxide produces mainly base oxidation but less bulky lesions; UVA/B generates a mixture of both. These findings reinforce the value of CA in testing in DNA repair ability or exposure monitoring. © 2014 Begell House, Inc. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07318898_v33_n4_p279_PrietoGonzalez http://hdl.handle.net/20.500.12110/paper_07318898_v33_n4_p279_PrietoGonzalez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Base excision repair (BER) Comet assay DNA repair mechanisms Hydrogen peroxide Nucleotide excision repair (NER) rUVA/B Xeroderma pigmentosum (XP) base DNA hydrogen peroxide sunscreen hydrogen peroxide adult Article blood sampling cancer susceptibility case report cell migration child clinical feature comet assay controlled study DNA repair excision repair female human human cell kinetics leukocyte lotion male nutritional counseling oxidation oxidative stress radiation exposure room temperature school child skin cancer skin defect sun exposure ultraviolet A radiation ultraviolet B radiation xeroderma pigmentosum chemically induced DNA damage drug effects genetics leukocyte metabolism pathology radiation response time toxicity ultraviolet radiation xeroderma pigmentosum Adult Child Comet Assay DNA Damage DNA Repair Female Humans Hydrogen Peroxide Kinetics Leukocytes Male Time Factors Ultraviolet Rays Xeroderma Pigmentosum |
| spellingShingle |
Base excision repair (BER) Comet assay DNA repair mechanisms Hydrogen peroxide Nucleotide excision repair (NER) rUVA/B Xeroderma pigmentosum (XP) base DNA hydrogen peroxide sunscreen hydrogen peroxide adult Article blood sampling cancer susceptibility case report cell migration child clinical feature comet assay controlled study DNA repair excision repair female human human cell kinetics leukocyte lotion male nutritional counseling oxidation oxidative stress radiation exposure room temperature school child skin cancer skin defect sun exposure ultraviolet A radiation ultraviolet B radiation xeroderma pigmentosum chemically induced DNA damage drug effects genetics leukocyte metabolism pathology radiation response time toxicity ultraviolet radiation xeroderma pigmentosum Adult Child Comet Assay DNA Damage DNA Repair Female Humans Hydrogen Peroxide Kinetics Leukocytes Male Time Factors Ultraviolet Rays Xeroderma Pigmentosum DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects |
| topic_facet |
Base excision repair (BER) Comet assay DNA repair mechanisms Hydrogen peroxide Nucleotide excision repair (NER) rUVA/B Xeroderma pigmentosum (XP) base DNA hydrogen peroxide sunscreen hydrogen peroxide adult Article blood sampling cancer susceptibility case report cell migration child clinical feature comet assay controlled study DNA repair excision repair female human human cell kinetics leukocyte lotion male nutritional counseling oxidation oxidative stress radiation exposure room temperature school child skin cancer skin defect sun exposure ultraviolet A radiation ultraviolet B radiation xeroderma pigmentosum chemically induced DNA damage drug effects genetics leukocyte metabolism pathology radiation response time toxicity ultraviolet radiation xeroderma pigmentosum Adult Child Comet Assay DNA Damage DNA Repair Female Humans Hydrogen Peroxide Kinetics Leukocytes Male Time Factors Ultraviolet Rays Xeroderma Pigmentosum |
| description |
The objective of the present work was to study the fine kinetics of DNA repair in xeroderma pigmentosum (XP) syndrome, a complex disorder linked to a deficiency in repair that increases cancer susceptibility. The repair process was evaluated by the comet assay (CA) in cells from 2 XP patients and 9 controls exposed to UVA/B (UVA 366/UVB 280 nm) and H2O2(150 μM) at temperatures of 4, 15, and 37°C. Samples were taken at 2-min intervals during the first 10 min to analyze the “fine kinetics” repair during the initial phase of the curve, and then at 15, 20, 25, 30, 45, 60, and 120 min. CA evaluation of DNA repair activity points to BER/NER initiation in the first 30 min with both inductors at 37°C and 15°C, but final comet length showed differences according to treatment. Repair kinetics during 120 min showed a good correlation with clinical features in both XP patients. Differences in final comet length were less pronounced in XP cells treated with H2O2than with UVA/B, probably because the peroxide produces mainly base oxidation but less bulky lesions; UVA/B generates a mixture of both. These findings reinforce the value of CA in testing in DNA repair ability or exposure monitoring. © 2014 Begell House, Inc. |
| title |
DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects |
| title_short |
DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects |
| title_full |
DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects |
| title_fullStr |
DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects |
| title_full_unstemmed |
DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects |
| title_sort |
dna repair kinetic of hydrogen peroxide and uva/b induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07318898_v33_n4_p279_PrietoGonzalez http://hdl.handle.net/20.500.12110/paper_07318898_v33_n4_p279_PrietoGonzalez |
| _version_ |
1768544645380308992 |