5-Aminolevulinic acid-mediated photodynamic therapy on Hep-2 and MCF-7c3 cells

The cytotoxic effect of 5-aminolevulinic acid (ALA) induced protoporphyrin IX (PPIX) on two human carcinoma cell lines, MCF-7c3 cells and Hep 2 cells, was studied. In both cell lines, PPIX content depends on the ALA concentration and incubation time. The maximal PPIX content was higher in the MCF-7c...

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Autores principales:	Batlle, Alcira María del Carmen, Fukuda, Haydeé
Publicado:	2007
Materias:	5-aminolevulinic acid Apoptosis Necrosis Photodynamic therapy Photosensitisation aminolevulinic acid deferoxamine iron chelating agent protoporphyrin analytic method apoptosis cancer cell cell death cell structure chromatin comparative study conference paper controlled study fluorescence microscopy human human cell incubation time lysosome photodynamic therapy priority journal radiation dose
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07318898_v26_n2_p75_Alvarez http://hdl.handle.net/20.500.12110/paper_07318898_v26_n2_p75_Alvarez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_07318898_v26_n2_p75_Alvarez
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spelling	paper:paper_07318898_v26_n2_p75_Alvarez2023-06-08T15:43:54Z 5-Aminolevulinic acid-mediated photodynamic therapy on Hep-2 and MCF-7c3 cells Batlle, Alcira María del Carmen Fukuda, Haydeé 5-aminolevulinic acid Apoptosis Necrosis Photodynamic therapy Photosensitisation aminolevulinic acid deferoxamine iron chelating agent protoporphyrin analytic method apoptosis cancer cell cell death cell structure chromatin comparative study conference paper controlled study fluorescence microscopy human human cell incubation time lysosome photodynamic therapy priority journal radiation dose The cytotoxic effect of 5-aminolevulinic acid (ALA) induced protoporphyrin IX (PPIX) on two human carcinoma cell lines, MCF-7c3 cells and Hep 2 cells, was studied. In both cell lines, PPIX content depends on the ALA concentration and incubation time. The maximal PPIX content was higher in the MCF-7c3 cells, reaching a value of 8 μg/106 cells, compared to the Hep-2 cells, which accumulated 3.2 μg/106 cells. Treatment of cells with the iron chelator desferrioxamine prior to ALA exposure enhances the amount of PPIX, consequently diminishing enzymatic activity of ferroquelatase. Photo sensitization of the cells was in correlation with the PPIX content; therefore, conditions leading to 80% cell death in the MCF-7c3 cells provoke a 50% cell death in the Hep 2 cells. Using fluorescence microscopy, cell morphology was analyzed after incubation with 1 mM ALA during 5 hr and irradiation with 54 Jcm-2; 24 hr post-PDT, MCF-7c3 cells revealed the typical morphological changes of necrosis. Under the same conditions, Hep-2 cells produced chromatine fragmentation characteristic of apoptosis. PPIX accumulation was observed to occur in a perinuclear region in the MCF-7c3 cells; while in Hep-2 cells, it was localized in lysosomes. Different mechanisms of cell death were observed in both cell lines, depending on the different intracellular localization of PPIX. © 2007 by Begell House, Inc. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fukuda, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07318898_v26_n2_p75_Alvarez http://hdl.handle.net/20.500.12110/paper_07318898_v26_n2_p75_Alvarez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	5-aminolevulinic acid Apoptosis Necrosis Photodynamic therapy Photosensitisation aminolevulinic acid deferoxamine iron chelating agent protoporphyrin analytic method apoptosis cancer cell cell death cell structure chromatin comparative study conference paper controlled study fluorescence microscopy human human cell incubation time lysosome photodynamic therapy priority journal radiation dose
spellingShingle	5-aminolevulinic acid Apoptosis Necrosis Photodynamic therapy Photosensitisation aminolevulinic acid deferoxamine iron chelating agent protoporphyrin analytic method apoptosis cancer cell cell death cell structure chromatin comparative study conference paper controlled study fluorescence microscopy human human cell incubation time lysosome photodynamic therapy priority journal radiation dose Batlle, Alcira María del Carmen Fukuda, Haydeé 5-Aminolevulinic acid-mediated photodynamic therapy on Hep-2 and MCF-7c3 cells
topic_facet	5-aminolevulinic acid Apoptosis Necrosis Photodynamic therapy Photosensitisation aminolevulinic acid deferoxamine iron chelating agent protoporphyrin analytic method apoptosis cancer cell cell death cell structure chromatin comparative study conference paper controlled study fluorescence microscopy human human cell incubation time lysosome photodynamic therapy priority journal radiation dose
description	The cytotoxic effect of 5-aminolevulinic acid (ALA) induced protoporphyrin IX (PPIX) on two human carcinoma cell lines, MCF-7c3 cells and Hep 2 cells, was studied. In both cell lines, PPIX content depends on the ALA concentration and incubation time. The maximal PPIX content was higher in the MCF-7c3 cells, reaching a value of 8 μg/106 cells, compared to the Hep-2 cells, which accumulated 3.2 μg/106 cells. Treatment of cells with the iron chelator desferrioxamine prior to ALA exposure enhances the amount of PPIX, consequently diminishing enzymatic activity of ferroquelatase. Photo sensitization of the cells was in correlation with the PPIX content; therefore, conditions leading to 80% cell death in the MCF-7c3 cells provoke a 50% cell death in the Hep 2 cells. Using fluorescence microscopy, cell morphology was analyzed after incubation with 1 mM ALA during 5 hr and irradiation with 54 Jcm-2; 24 hr post-PDT, MCF-7c3 cells revealed the typical morphological changes of necrosis. Under the same conditions, Hep-2 cells produced chromatine fragmentation characteristic of apoptosis. PPIX accumulation was observed to occur in a perinuclear region in the MCF-7c3 cells; while in Hep-2 cells, it was localized in lysosomes. Different mechanisms of cell death were observed in both cell lines, depending on the different intracellular localization of PPIX. © 2007 by Begell House, Inc.
author	Batlle, Alcira María del Carmen Fukuda, Haydeé
author_facet	Batlle, Alcira María del Carmen Fukuda, Haydeé
author_sort	Batlle, Alcira María del Carmen
title	5-Aminolevulinic acid-mediated photodynamic therapy on Hep-2 and MCF-7c3 cells
title_short	5-Aminolevulinic acid-mediated photodynamic therapy on Hep-2 and MCF-7c3 cells
title_full	5-Aminolevulinic acid-mediated photodynamic therapy on Hep-2 and MCF-7c3 cells
title_fullStr	5-Aminolevulinic acid-mediated photodynamic therapy on Hep-2 and MCF-7c3 cells
title_full_unstemmed	5-Aminolevulinic acid-mediated photodynamic therapy on Hep-2 and MCF-7c3 cells
title_sort	5-aminolevulinic acid-mediated photodynamic therapy on hep-2 and mcf-7c3 cells
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07318898_v26_n2_p75_Alvarez http://hdl.handle.net/20.500.12110/paper_07318898_v26_n2_p75_Alvarez
work_keys_str_mv	AT batllealciramariadelcarmen 5aminolevulinicacidmediatedphotodynamictherapyonhep2andmcf7c3cells AT fukudahaydee 5aminolevulinicacidmediatedphotodynamictherapyonhep2andmcf7c3cells
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5-Aminolevulinic acid-mediated photodynamic therapy on Hep-2 and MCF-7c3 cells

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