ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes

The expression of receptors belonging to the epidermal growth factor receptor subfamily has been largely studied these last years in epithelial cells mainly as involved in cell proliferation and malignant progression. Although much work has focused on the role of these growth factor receptors in the...

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Autores principales:	Pagano, Eleonora, Calvo, Juan Carlos
Publicado:	2003
Materias:	3T3-L1 cells Adipocyte differentiation CAMP Dexamethasone EGF EGFR ErbB2 Fibroblasts Heregulin Insulin Tyrosine phosphorylation dexamethasone epidermal growth factor receptor growth factor receptor growth hormone heregulin alpha1 heregulin beta1 methylxanthine derivative neu differentiation factor thyroid hormone unclassified drug adipocyte animal cell article cell differentiation cell migration cell proliferation controlled study epithelium cell malignant transformation mitogenesis nonhuman oncogene neu polyacrylamide gel electrophoresis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 Cells Adipocytes Animals Cell Differentiation Epidermal Growth Factor Humans Mice Neuregulin-1 Phosphorylation Receptor, Epidermal Growth Factor Receptor, erbB-2 Tumor Cells, Cultured Murinae
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07302312_v90_n3_p561_Pagano http://hdl.handle.net/20.500.12110/paper_07302312_v90_n3_p561_Pagano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_07302312_v90_n3_p561_Pagano
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spelling	paper:paper_07302312_v90_n3_p561_Pagano2023-06-08T15:43:46Z ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes Pagano, Eleonora Calvo, Juan Carlos 3T3-L1 cells Adipocyte differentiation CAMP Dexamethasone EGF EGFR ErbB2 Fibroblasts Heregulin Insulin Tyrosine phosphorylation dexamethasone epidermal growth factor receptor growth factor receptor growth hormone heregulin alpha1 heregulin beta1 methylxanthine derivative neu differentiation factor thyroid hormone unclassified drug adipocyte animal cell article cell differentiation cell migration cell proliferation controlled study epithelium cell malignant transformation mitogenesis nonhuman oncogene neu polyacrylamide gel electrophoresis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 Cells Adipocytes Animals Cell Differentiation Dexamethasone Epidermal Growth Factor Humans Insulin Mice Neuregulin-1 Phosphorylation Receptor, Epidermal Growth Factor Receptor, erbB-2 Tumor Cells, Cultured Murinae The expression of receptors belonging to the epidermal growth factor receptor subfamily has been largely studied these last years in epithelial cells mainly as involved in cell proliferation and malignant progression. Although much work has focused on the role of these growth factor receptors in the differentiation of a variety of tissues, there is little information in regards to normal stromal cells. We investigated erbB2 expression in the murine fibroblast cell line Swiss 3T3L1, which naturally or hormonally induced undergoes adipocyte differentiation. We found that the Swiss 3T3-L1 fibroblasts express erbB2, in addition to EGFR, and in a quantity comparable to or even greater than the breast cancer cell line T47D. Proliferating cells increased erbB2 and EGFR levels when reaching confluence up to 4- and 10-fold, respectively. This expression showed a significant decrease when growth-arrested cells were stimulated to differentiate with dexamethasone and isobutyl-methylxanthine. Differentiated cells presented a decreased expression of both erbB2 and EGFR regardless of whether the cells were hormonally or spontaneously differentiated. EGF stimulation of serum-starved cells increased erbB2 tyrosine phosphorylation and retarded erbB2 migration in SDS-PAGE, suggesting receptor association and activation. Heregulin-α1 and β1, two EGF related factors, had no effect on erbB2 or EGFR phosphorylation. Although 3T3-L1 cells expressed heregulin, its specific receptors, erbB3 and erbB4, were not found. This is the first time in which erbB2 is reported to be expressed in an adipocytic cell line which does not depend on non EGF family growth factors (thyroid hormone, growth hormone, etc.) to accomplish adipose differentiation. Since erbB2 and EGFR expression were downmodulated as differentiation progressed it is conceivable that a mechanism of switching from a mitogenic to a differentiating signaling pathway may be involved, through regulation of the expression of these growth factor receptors. © 2003 Wiley-Liss, Inc. Fil:Pagano, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07302312_v90_n3_p561_Pagano http://hdl.handle.net/20.500.12110/paper_07302312_v90_n3_p561_Pagano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	3T3-L1 cells Adipocyte differentiation CAMP Dexamethasone EGF EGFR ErbB2 Fibroblasts Heregulin Insulin Tyrosine phosphorylation dexamethasone epidermal growth factor receptor growth factor receptor growth hormone heregulin alpha1 heregulin beta1 methylxanthine derivative neu differentiation factor thyroid hormone unclassified drug adipocyte animal cell article cell differentiation cell migration cell proliferation controlled study epithelium cell malignant transformation mitogenesis nonhuman oncogene neu polyacrylamide gel electrophoresis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 Cells Adipocytes Animals Cell Differentiation Dexamethasone Epidermal Growth Factor Humans Insulin Mice Neuregulin-1 Phosphorylation Receptor, Epidermal Growth Factor Receptor, erbB-2 Tumor Cells, Cultured Murinae
spellingShingle	3T3-L1 cells Adipocyte differentiation CAMP Dexamethasone EGF EGFR ErbB2 Fibroblasts Heregulin Insulin Tyrosine phosphorylation dexamethasone epidermal growth factor receptor growth factor receptor growth hormone heregulin alpha1 heregulin beta1 methylxanthine derivative neu differentiation factor thyroid hormone unclassified drug adipocyte animal cell article cell differentiation cell migration cell proliferation controlled study epithelium cell malignant transformation mitogenesis nonhuman oncogene neu polyacrylamide gel electrophoresis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 Cells Adipocytes Animals Cell Differentiation Dexamethasone Epidermal Growth Factor Humans Insulin Mice Neuregulin-1 Phosphorylation Receptor, Epidermal Growth Factor Receptor, erbB-2 Tumor Cells, Cultured Murinae Pagano, Eleonora Calvo, Juan Carlos ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes
topic_facet	3T3-L1 cells Adipocyte differentiation CAMP Dexamethasone EGF EGFR ErbB2 Fibroblasts Heregulin Insulin Tyrosine phosphorylation dexamethasone epidermal growth factor receptor growth factor receptor growth hormone heregulin alpha1 heregulin beta1 methylxanthine derivative neu differentiation factor thyroid hormone unclassified drug adipocyte animal cell article cell differentiation cell migration cell proliferation controlled study epithelium cell malignant transformation mitogenesis nonhuman oncogene neu polyacrylamide gel electrophoresis priority journal proadipocyte protein expression protein phosphorylation 3T3-L1 Cells Adipocytes Animals Cell Differentiation Dexamethasone Epidermal Growth Factor Humans Insulin Mice Neuregulin-1 Phosphorylation Receptor, Epidermal Growth Factor Receptor, erbB-2 Tumor Cells, Cultured Murinae
description	The expression of receptors belonging to the epidermal growth factor receptor subfamily has been largely studied these last years in epithelial cells mainly as involved in cell proliferation and malignant progression. Although much work has focused on the role of these growth factor receptors in the differentiation of a variety of tissues, there is little information in regards to normal stromal cells. We investigated erbB2 expression in the murine fibroblast cell line Swiss 3T3L1, which naturally or hormonally induced undergoes adipocyte differentiation. We found that the Swiss 3T3-L1 fibroblasts express erbB2, in addition to EGFR, and in a quantity comparable to or even greater than the breast cancer cell line T47D. Proliferating cells increased erbB2 and EGFR levels when reaching confluence up to 4- and 10-fold, respectively. This expression showed a significant decrease when growth-arrested cells were stimulated to differentiate with dexamethasone and isobutyl-methylxanthine. Differentiated cells presented a decreased expression of both erbB2 and EGFR regardless of whether the cells were hormonally or spontaneously differentiated. EGF stimulation of serum-starved cells increased erbB2 tyrosine phosphorylation and retarded erbB2 migration in SDS-PAGE, suggesting receptor association and activation. Heregulin-α1 and β1, two EGF related factors, had no effect on erbB2 or EGFR phosphorylation. Although 3T3-L1 cells expressed heregulin, its specific receptors, erbB3 and erbB4, were not found. This is the first time in which erbB2 is reported to be expressed in an adipocytic cell line which does not depend on non EGF family growth factors (thyroid hormone, growth hormone, etc.) to accomplish adipose differentiation. Since erbB2 and EGFR expression were downmodulated as differentiation progressed it is conceivable that a mechanism of switching from a mitogenic to a differentiating signaling pathway may be involved, through regulation of the expression of these growth factor receptors. © 2003 Wiley-Liss, Inc.
author	Pagano, Eleonora Calvo, Juan Carlos
author_facet	Pagano, Eleonora Calvo, Juan Carlos
author_sort	Pagano, Eleonora
title	ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes
title_short	ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes
title_full	ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes
title_fullStr	ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes
title_full_unstemmed	ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes
title_sort	erbb2 and egfr are downmodulated during the differentiation of 3t3-l1 preadipocytes
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07302312_v90_n3_p561_Pagano http://hdl.handle.net/20.500.12110/paper_07302312_v90_n3_p561_Pagano
work_keys_str_mv	AT paganoeleonora erbb2andegfraredownmodulatedduringthedifferentiationof3t3l1preadipocytes AT calvojuancarlos erbb2andegfraredownmodulatedduringthedifferentiationof3t3l1preadipocytes
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ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes

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