Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin
The widespread use of aluminum (Al) provides easy exposure of humans to the metal and its accumulation remains a potential problem. In vivo and in vitro assays have associated Al overload with anemia. To better understand the mechanisms by which Al affects human erythrocytes, morphological and bioch...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07302312_v113_n5_p1581_Vota http://hdl.handle.net/20.500.12110/paper_07302312_v113_n5_p1581_Vota |
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paper:paper_07302312_v113_n5_p1581_Vota2023-06-08T15:43:44Z Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin aluminum erythrocytes erythropoietin oxidative stress acetylcysteine aluminum calcium glutathione phosphatidylserine reactive oxygen metabolite recombinant erythropoietin adult anemia article calcium cell level cell death cell membrane cell protection cell shape cell structure cell surface controlled study eryptosis erythrocyte erythroid cell human human cell long term exposure normal human oxidation reduction reaction oxidative stress priority journal protein degradation Adult Aluminum Anemia Anion Exchange Protein 1, Erythrocyte Erythrocyte Aging Erythrocyte Membrane Erythrocytes Erythropoietin Humans Microscopy, Electron, Scanning Oxidative Stress Recombinant Proteins The widespread use of aluminum (Al) provides easy exposure of humans to the metal and its accumulation remains a potential problem. In vivo and in vitro assays have associated Al overload with anemia. To better understand the mechanisms by which Al affects human erythrocytes, morphological and biochemical changes were analyzed after long-term treatment using an in vitro model. The appearance of erythrocytes with abnormal shapes suggested metal interaction with cell surface, supported by the fact that high amounts of Al attached to cell membrane. Long-term incubation of human erythrocytes with Al induced signs of premature erythrocyte death (eryptosis), such as phosphatidylserine externalization, increased intracellular calcium, and band 3 degradation. Signs of oxidative stress, such as significant increase in reactive oxygen species in parallel with decrease in the amount of reduced glutathione, were also observed. These oxidative effects were completely prevented by the antioxidant N-acetylcysteine. Interestingly, erythrocytes were also protected from the prooxidative action of Al by the presence of erythropoietin (EPO). In conclusion, results provide evidence that chronic Al exposure may lead to biochemical and morphological alterations similar to those shown in eryptosis induced by oxidant compounds in human erythrocytes. The antieryptotic effect of EPO may contribute to enhance the knowledge of its physiological role on erythroid cells. Irrespective of the antioxidant mechanism, this property of EPO, shown in this model of Al exposure, let us suggest potential benefits by EPO treatment of patients with anemia associated to altered redox environment. © 2011 Wiley Periodicals, Inc. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07302312_v113_n5_p1581_Vota http://hdl.handle.net/20.500.12110/paper_07302312_v113_n5_p1581_Vota |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
aluminum erythrocytes erythropoietin oxidative stress acetylcysteine aluminum calcium glutathione phosphatidylserine reactive oxygen metabolite recombinant erythropoietin adult anemia article calcium cell level cell death cell membrane cell protection cell shape cell structure cell surface controlled study eryptosis erythrocyte erythroid cell human human cell long term exposure normal human oxidation reduction reaction oxidative stress priority journal protein degradation Adult Aluminum Anemia Anion Exchange Protein 1, Erythrocyte Erythrocyte Aging Erythrocyte Membrane Erythrocytes Erythropoietin Humans Microscopy, Electron, Scanning Oxidative Stress Recombinant Proteins |
| spellingShingle |
aluminum erythrocytes erythropoietin oxidative stress acetylcysteine aluminum calcium glutathione phosphatidylserine reactive oxygen metabolite recombinant erythropoietin adult anemia article calcium cell level cell death cell membrane cell protection cell shape cell structure cell surface controlled study eryptosis erythrocyte erythroid cell human human cell long term exposure normal human oxidation reduction reaction oxidative stress priority journal protein degradation Adult Aluminum Anemia Anion Exchange Protein 1, Erythrocyte Erythrocyte Aging Erythrocyte Membrane Erythrocytes Erythropoietin Humans Microscopy, Electron, Scanning Oxidative Stress Recombinant Proteins Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin |
| topic_facet |
aluminum erythrocytes erythropoietin oxidative stress acetylcysteine aluminum calcium glutathione phosphatidylserine reactive oxygen metabolite recombinant erythropoietin adult anemia article calcium cell level cell death cell membrane cell protection cell shape cell structure cell surface controlled study eryptosis erythrocyte erythroid cell human human cell long term exposure normal human oxidation reduction reaction oxidative stress priority journal protein degradation Adult Aluminum Anemia Anion Exchange Protein 1, Erythrocyte Erythrocyte Aging Erythrocyte Membrane Erythrocytes Erythropoietin Humans Microscopy, Electron, Scanning Oxidative Stress Recombinant Proteins |
| description |
The widespread use of aluminum (Al) provides easy exposure of humans to the metal and its accumulation remains a potential problem. In vivo and in vitro assays have associated Al overload with anemia. To better understand the mechanisms by which Al affects human erythrocytes, morphological and biochemical changes were analyzed after long-term treatment using an in vitro model. The appearance of erythrocytes with abnormal shapes suggested metal interaction with cell surface, supported by the fact that high amounts of Al attached to cell membrane. Long-term incubation of human erythrocytes with Al induced signs of premature erythrocyte death (eryptosis), such as phosphatidylserine externalization, increased intracellular calcium, and band 3 degradation. Signs of oxidative stress, such as significant increase in reactive oxygen species in parallel with decrease in the amount of reduced glutathione, were also observed. These oxidative effects were completely prevented by the antioxidant N-acetylcysteine. Interestingly, erythrocytes were also protected from the prooxidative action of Al by the presence of erythropoietin (EPO). In conclusion, results provide evidence that chronic Al exposure may lead to biochemical and morphological alterations similar to those shown in eryptosis induced by oxidant compounds in human erythrocytes. The antieryptotic effect of EPO may contribute to enhance the knowledge of its physiological role on erythroid cells. Irrespective of the antioxidant mechanism, this property of EPO, shown in this model of Al exposure, let us suggest potential benefits by EPO treatment of patients with anemia associated to altered redox environment. © 2011 Wiley Periodicals, Inc. |
| title |
Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin |
| title_short |
Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin |
| title_full |
Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin |
| title_fullStr |
Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin |
| title_full_unstemmed |
Oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin |
| title_sort |
oxidative stress due to aluminum exposure induces eryptosis which is prevented by erythropoietin |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07302312_v113_n5_p1581_Vota http://hdl.handle.net/20.500.12110/paper_07302312_v113_n5_p1581_Vota |
| _version_ |
1768543233495793664 |