Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)
The promoter region of two sunflower (Helianthus annuus L. HA89 genotype) seed specifically expressed genes, coding for an oleate desaturase (HaFAD2-1) and a lipid transfer protein (HaAP10), were cloned and in silico characterized. The isolated fragments are 867 and 964 bp long, respectively, and co...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07217714_v29_n3_p239_Zavallo http://hdl.handle.net/20.500.12110/paper_07217714_v29_n3_p239_Zavallo |
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paper:paper_07217714_v29_n3_p239_Zavallo2023-06-08T15:43:22Z Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.) Zavallo, Diego Hopp, Horacio Esteban Heinz, Ruth Amelia Lipid transfer protein Oleate desaturase Seed promoter Sunflower Arabidopsis Helianthus Helianthus annuus acyl coenzyme A desaturase carrier protein lipid transfer protein oxidoreductase Phosphatidylcholine desaturase plant RNA vegetable protein Arabidopsis article gene expression profiling gene expression regulation genetics molecular cloning plant gene plant seed promoter region sunflower transgenic plant Arabidopsis Carrier Proteins Cloning, Molecular Fatty Acid Desaturases Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Helianthus Oxidoreductases Acting on CH-CH Group Donors Plant Proteins Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant Seeds The promoter region of two sunflower (Helianthus annuus L. HA89 genotype) seed specifically expressed genes, coding for an oleate desaturase (HaFAD2-1) and a lipid transfer protein (HaAP10), were cloned and in silico characterized. The isolated fragments are 867 and 964 bp long, respectively, and contain several seed-specific motifs, such as AACA motif, ACGT element, E-Boxes, SEF binding sites and GCN4 motif. Functional analysis of these promoters in transgenic Arabidopsis plants was investigated after fusing them with the β-glucuronidase (GUS) reporter gene. None of the promoters triggered GUS activity in any vegetative tissue, with the exception of early seedling cotyledons. HaFAD2-1 and HaAP10 promoters were tested along seed development from globular stage to mature seeds. GUS staining was restricted to embryonic tissue and quantitative fluorometric assays showed high activity values at the later stages of development. In this work we demonstrate that HaFAD2-1 promoter is as strong as 35S promoter even though it is a tissue-specific promoter and its activity derived just from the embryo, thus confirming that it can be considered a strong highly specific seed promoter useful for biotechnology applications. © 2010 Springer-Verlag. Fil:Zavallo, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Heinz, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07217714_v29_n3_p239_Zavallo http://hdl.handle.net/20.500.12110/paper_07217714_v29_n3_p239_Zavallo |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Lipid transfer protein Oleate desaturase Seed promoter Sunflower Arabidopsis Helianthus Helianthus annuus acyl coenzyme A desaturase carrier protein lipid transfer protein oxidoreductase Phosphatidylcholine desaturase plant RNA vegetable protein Arabidopsis article gene expression profiling gene expression regulation genetics molecular cloning plant gene plant seed promoter region sunflower transgenic plant Arabidopsis Carrier Proteins Cloning, Molecular Fatty Acid Desaturases Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Helianthus Oxidoreductases Acting on CH-CH Group Donors Plant Proteins Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant Seeds |
spellingShingle |
Lipid transfer protein Oleate desaturase Seed promoter Sunflower Arabidopsis Helianthus Helianthus annuus acyl coenzyme A desaturase carrier protein lipid transfer protein oxidoreductase Phosphatidylcholine desaturase plant RNA vegetable protein Arabidopsis article gene expression profiling gene expression regulation genetics molecular cloning plant gene plant seed promoter region sunflower transgenic plant Arabidopsis Carrier Proteins Cloning, Molecular Fatty Acid Desaturases Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Helianthus Oxidoreductases Acting on CH-CH Group Donors Plant Proteins Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant Seeds Zavallo, Diego Hopp, Horacio Esteban Heinz, Ruth Amelia Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.) |
topic_facet |
Lipid transfer protein Oleate desaturase Seed promoter Sunflower Arabidopsis Helianthus Helianthus annuus acyl coenzyme A desaturase carrier protein lipid transfer protein oxidoreductase Phosphatidylcholine desaturase plant RNA vegetable protein Arabidopsis article gene expression profiling gene expression regulation genetics molecular cloning plant gene plant seed promoter region sunflower transgenic plant Arabidopsis Carrier Proteins Cloning, Molecular Fatty Acid Desaturases Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Helianthus Oxidoreductases Acting on CH-CH Group Donors Plant Proteins Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant Seeds |
description |
The promoter region of two sunflower (Helianthus annuus L. HA89 genotype) seed specifically expressed genes, coding for an oleate desaturase (HaFAD2-1) and a lipid transfer protein (HaAP10), were cloned and in silico characterized. The isolated fragments are 867 and 964 bp long, respectively, and contain several seed-specific motifs, such as AACA motif, ACGT element, E-Boxes, SEF binding sites and GCN4 motif. Functional analysis of these promoters in transgenic Arabidopsis plants was investigated after fusing them with the β-glucuronidase (GUS) reporter gene. None of the promoters triggered GUS activity in any vegetative tissue, with the exception of early seedling cotyledons. HaFAD2-1 and HaAP10 promoters were tested along seed development from globular stage to mature seeds. GUS staining was restricted to embryonic tissue and quantitative fluorometric assays showed high activity values at the later stages of development. In this work we demonstrate that HaFAD2-1 promoter is as strong as 35S promoter even though it is a tissue-specific promoter and its activity derived just from the embryo, thus confirming that it can be considered a strong highly specific seed promoter useful for biotechnology applications. © 2010 Springer-Verlag. |
author |
Zavallo, Diego Hopp, Horacio Esteban Heinz, Ruth Amelia |
author_facet |
Zavallo, Diego Hopp, Horacio Esteban Heinz, Ruth Amelia |
author_sort |
Zavallo, Diego |
title |
Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.) |
title_short |
Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.) |
title_full |
Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.) |
title_fullStr |
Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.) |
title_full_unstemmed |
Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.) |
title_sort |
isolation and functional characterization of two novel seed-specific promoters from sunflower (helianthus annuus l.) |
publishDate |
2010 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07217714_v29_n3_p239_Zavallo http://hdl.handle.net/20.500.12110/paper_07217714_v29_n3_p239_Zavallo |
work_keys_str_mv |
AT zavallodiego isolationandfunctionalcharacterizationoftwonovelseedspecificpromotersfromsunflowerhelianthusannuusl AT hopphoracioesteban isolationandfunctionalcharacterizationoftwonovelseedspecificpromotersfromsunflowerhelianthusannuusl AT heinzruthamelia isolationandfunctionalcharacterizationoftwonovelseedspecificpromotersfromsunflowerhelianthusannuusl |
_version_ |
1768544456183644160 |