Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map
In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the ident...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07173458_v13_n6_p_Talia http://hdl.handle.net/20.500.12110/paper_07173458_v13_n6_p_Talia |
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paper:paper_07173458_v13_n6_p_Talia2023-06-08T15:43:01Z Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map Talia, Paola M. Hopp, Horacio Esteban Heinz, Ruth Amelia EST-SSR InDels Linkage map SSR Sunflower EST-SSR InDels Linkage map SSR Sunflower Plants (botany) Genes agronomic trait amplified fragment length polymorphism article controlled study expressed sequence tag gene deletion gene insertion gene locus gene mapping genetic database genetic linkage genetic recombination genetic trait inbred strain linkage analysis nonhuman nucleotide sequence plant gene polymerase chain reaction simple sequence repeat sunflower Helianthus In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the identification and characterization of 1,134 simple sequence repeat (SSR) containing expressed sequence tags (ESTs) from unigenes available databases. Twelve of these functional markers as well as 41 public SSR markers were successfully localized in linkage groups, thus contributing to the saturation of specific regions on a reference genetic-linkage-map derived from recombinant inbred lines (RIL) mapping population from the cross between PAC2 x RHA266 lines. The enriched map includes 547 markers (231 SSR, 9 EST-SSR, 3 insertions/deletions (InDels) and 304 amplified fragment length polymorphisms (AFLPs) distributed in 17 linkage groups (LG), spanning genetic size to 1,942.3 cM and improving its mean density to 3.6 cM per locus. As consequence, no gaps longer than 13.2 cM remain uncovered throughout the entire map, which increases the feasibility of detecting genes or traits of agronomic importance in sunflower. © 2010 by Pontificia Universidad Católica de Valparaíso, Chile. Fil:Talia, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Heinz, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07173458_v13_n6_p_Talia http://hdl.handle.net/20.500.12110/paper_07173458_v13_n6_p_Talia |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
EST-SSR InDels Linkage map SSR Sunflower EST-SSR InDels Linkage map SSR Sunflower Plants (botany) Genes agronomic trait amplified fragment length polymorphism article controlled study expressed sequence tag gene deletion gene insertion gene locus gene mapping genetic database genetic linkage genetic recombination genetic trait inbred strain linkage analysis nonhuman nucleotide sequence plant gene polymerase chain reaction simple sequence repeat sunflower Helianthus |
spellingShingle |
EST-SSR InDels Linkage map SSR Sunflower EST-SSR InDels Linkage map SSR Sunflower Plants (botany) Genes agronomic trait amplified fragment length polymorphism article controlled study expressed sequence tag gene deletion gene insertion gene locus gene mapping genetic database genetic linkage genetic recombination genetic trait inbred strain linkage analysis nonhuman nucleotide sequence plant gene polymerase chain reaction simple sequence repeat sunflower Helianthus Talia, Paola M. Hopp, Horacio Esteban Heinz, Ruth Amelia Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map |
topic_facet |
EST-SSR InDels Linkage map SSR Sunflower EST-SSR InDels Linkage map SSR Sunflower Plants (botany) Genes agronomic trait amplified fragment length polymorphism article controlled study expressed sequence tag gene deletion gene insertion gene locus gene mapping genetic database genetic linkage genetic recombination genetic trait inbred strain linkage analysis nonhuman nucleotide sequence plant gene polymerase chain reaction simple sequence repeat sunflower Helianthus |
description |
In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the identification and characterization of 1,134 simple sequence repeat (SSR) containing expressed sequence tags (ESTs) from unigenes available databases. Twelve of these functional markers as well as 41 public SSR markers were successfully localized in linkage groups, thus contributing to the saturation of specific regions on a reference genetic-linkage-map derived from recombinant inbred lines (RIL) mapping population from the cross between PAC2 x RHA266 lines. The enriched map includes 547 markers (231 SSR, 9 EST-SSR, 3 insertions/deletions (InDels) and 304 amplified fragment length polymorphisms (AFLPs) distributed in 17 linkage groups (LG), spanning genetic size to 1,942.3 cM and improving its mean density to 3.6 cM per locus. As consequence, no gaps longer than 13.2 cM remain uncovered throughout the entire map, which increases the feasibility of detecting genes or traits of agronomic importance in sunflower. © 2010 by Pontificia Universidad Católica de Valparaíso, Chile. |
author |
Talia, Paola M. Hopp, Horacio Esteban Heinz, Ruth Amelia |
author_facet |
Talia, Paola M. Hopp, Horacio Esteban Heinz, Ruth Amelia |
author_sort |
Talia, Paola M. |
title |
Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map |
title_short |
Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map |
title_full |
Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map |
title_fullStr |
Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map |
title_full_unstemmed |
Genetic mapping of EST-SSRs, SSR and InDels to improve saturation of genomic regions in a previously developed sunflower map |
title_sort |
genetic mapping of est-ssrs, ssr and indels to improve saturation of genomic regions in a previously developed sunflower map |
publishDate |
2010 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07173458_v13_n6_p_Talia http://hdl.handle.net/20.500.12110/paper_07173458_v13_n6_p_Talia |
work_keys_str_mv |
AT taliapaolam geneticmappingofestssrsssrandindelstoimprovesaturationofgenomicregionsinapreviouslydevelopedsunflowermap AT hopphoracioesteban geneticmappingofestssrsssrandindelstoimprovesaturationofgenomicregionsinapreviouslydevelopedsunflowermap AT heinzruthamelia geneticmappingofestssrsssrandindelstoimprovesaturationofgenomicregionsinapreviouslydevelopedsunflowermap |
_version_ |
1768544230091784192 |