Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS

Soluble sugars play a central role in metabolism and water relations, their analysis can provide important information about biochemistry of growth, flowering and exposure of plants to different stresses. Here we report a successful application of UV-MALDI-MS for direct profiling of soluble sugars o...

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Guardado en:
Detalles Bibliográficos
Publicado: 2011
Materias:
Mass spectrometry
Soluble carbohydrate profiling
Sugar
Tulipa
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_05677572_v923_n_p53_Nonami
http://hdl.handle.net/20.500.12110/paper_05677572_v923_n_p53_Nonami
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_05677572_v923_n_p53_Nonami
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spelling paper:paper_05677572_v923_n_p53_Nonami2023-06-08T15:42:37Z Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS Mass spectrometry Soluble carbohydrate profiling Sugar Tulipa Soluble sugars play a central role in metabolism and water relations, their analysis can provide important information about biochemistry of growth, flowering and exposure of plants to different stresses. Here we report a successful application of UV-MALDI-MS for direct profiling of soluble sugars on the surface of tulip bulb tissue and also their detection in microsamples sucked from single cells of tulip bulbs by using Pressure Probe. Soluble neutral carbohydrates could be directly desorbed/ ionized by deposition of UV-MALDI matrix on the dried tissue. Despite the tiny volume of the single cell microsample sucked (picoliter) the high sensitivity of UVMALDI- MS allowed the detection of soluble sugars after its deposition on air-dried layers of different matrixes. Results obtained from intact tissue and single cell level experiments were comparable; similar sugars signals could be detected and also their relative signal intensity were similar in both experiments. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_05677572_v923_n_p53_Nonami http://hdl.handle.net/20.500.12110/paper_05677572_v923_n_p53_Nonami
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Mass spectrometry
Soluble carbohydrate profiling
Sugar
Tulipa
spellingShingle Mass spectrometry
Soluble carbohydrate profiling
Sugar
Tulipa
Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS
topic_facet Mass spectrometry
Soluble carbohydrate profiling
Sugar
Tulipa
description Soluble sugars play a central role in metabolism and water relations, their analysis can provide important information about biochemistry of growth, flowering and exposure of plants to different stresses. Here we report a successful application of UV-MALDI-MS for direct profiling of soluble sugars on the surface of tulip bulb tissue and also their detection in microsamples sucked from single cells of tulip bulbs by using Pressure Probe. Soluble neutral carbohydrates could be directly desorbed/ ionized by deposition of UV-MALDI matrix on the dried tissue. Despite the tiny volume of the single cell microsample sucked (picoliter) the high sensitivity of UVMALDI- MS allowed the detection of soluble sugars after its deposition on air-dried layers of different matrixes. Results obtained from intact tissue and single cell level experiments were comparable; similar sugars signals could be detected and also their relative signal intensity were similar in both experiments.
title Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS
title_short Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS
title_full Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS
title_fullStr Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS
title_full_unstemmed Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS
title_sort direct tissue and cell analysis of tulip bulbs by combining pressure probe and uv-maldi-ms
publishDate 2011
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_05677572_v923_n_p53_Nonami
http://hdl.handle.net/20.500.12110/paper_05677572_v923_n_p53_Nonami
_version_ 1768546447604580352

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