Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad

A novel biospecific affinity chromatographic procedure was developed for the purification of the sex steroid binding protein from Bufo arenarum. A charcoal column connected in series to the affinity column allows the removal of any ligand non-covalently bound to the matrix or released during its sto...

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Autores principales:	Santa Coloma, Tomás Antonio, Garraffo, Hugo Martín, Gros, Eduardo Gervasio, Charreau, Eduardo Hernán
Publicado:	1987
Materias:	sex hormone binding protein affinity chromatography animal cell methodology nonhuman priority journal protein purification toad Ammonium Sulfate Androstane-3,17-diol Animal Bufo arenarum Carrier Proteins Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Indicators and Reagents Protein Binding Sex Hormone-Binding Globulin Stanolone
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03784347_v415_nC_p297_SantaColoma http://hdl.handle.net/20.500.12110/paper_03784347_v415_nC_p297_SantaColoma
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03784347_v415_nC_p297_SantaColoma
record_format	dspace
spelling	paper:paper_03784347_v415_nC_p297_SantaColoma2023-06-08T15:39:45Z Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad Santa Coloma, Tomás Antonio Garraffo, Hugo Martín Gros, Eduardo Gervasio Charreau, Eduardo Hernán sex hormone binding protein affinity chromatography animal cell methodology nonhuman priority journal protein purification toad Ammonium Sulfate Androstane-3,17-diol Animal Bufo arenarum Carrier Proteins Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Indicators and Reagents Protein Binding Sex Hormone-Binding Globulin Stanolone A novel biospecific affinity chromatographic procedure was developed for the purification of the sex steroid binding protein from Bufo arenarum. A charcoal column connected in series to the affinity column allows the removal of any ligand non-covalently bound to the matrix or released during its storage, thus avoiding the need for exhaustive and prolonged washing procedures. In addition, it is not necessary to remove the endogenous ligand from the starting material and the binding to the affinity column can be monitored to determine the time required to achieve the maximum yield. The advantages are the charcoal adsorption of the ligand "washed" from the affinity column by the protein to be purified and the amplification provided by the cyclic use of the system. The procedure improves the yield from less than 1% (by conventional procedures) to more than 50%. With minor modifications this procedure can be useful for the purification of binding proteins and receptors. © 1987. Fil:Santa-Coloma, T.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Garraffo, H.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gros, E.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1987 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03784347_v415_nC_p297_SantaColoma http://hdl.handle.net/20.500.12110/paper_03784347_v415_nC_p297_SantaColoma
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	sex hormone binding protein affinity chromatography animal cell methodology nonhuman priority journal protein purification toad Ammonium Sulfate Androstane-3,17-diol Animal Bufo arenarum Carrier Proteins Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Indicators and Reagents Protein Binding Sex Hormone-Binding Globulin Stanolone
spellingShingle	sex hormone binding protein affinity chromatography animal cell methodology nonhuman priority journal protein purification toad Ammonium Sulfate Androstane-3,17-diol Animal Bufo arenarum Carrier Proteins Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Indicators and Reagents Protein Binding Sex Hormone-Binding Globulin Stanolone Santa Coloma, Tomás Antonio Garraffo, Hugo Martín Gros, Eduardo Gervasio Charreau, Eduardo Hernán Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
topic_facet	sex hormone binding protein affinity chromatography animal cell methodology nonhuman priority journal protein purification toad Ammonium Sulfate Androstane-3,17-diol Animal Bufo arenarum Carrier Proteins Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Indicators and Reagents Protein Binding Sex Hormone-Binding Globulin Stanolone
description	A novel biospecific affinity chromatographic procedure was developed for the purification of the sex steroid binding protein from Bufo arenarum. A charcoal column connected in series to the affinity column allows the removal of any ligand non-covalently bound to the matrix or released during its storage, thus avoiding the need for exhaustive and prolonged washing procedures. In addition, it is not necessary to remove the endogenous ligand from the starting material and the binding to the affinity column can be monitored to determine the time required to achieve the maximum yield. The advantages are the charcoal adsorption of the ligand "washed" from the affinity column by the protein to be purified and the amplification provided by the cyclic use of the system. The procedure improves the yield from less than 1% (by conventional procedures) to more than 50%. With minor modifications this procedure can be useful for the purification of binding proteins and receptors. © 1987.
author	Santa Coloma, Tomás Antonio Garraffo, Hugo Martín Gros, Eduardo Gervasio Charreau, Eduardo Hernán
author_facet	Santa Coloma, Tomás Antonio Garraffo, Hugo Martín Gros, Eduardo Gervasio Charreau, Eduardo Hernán
author_sort	Santa Coloma, Tomás Antonio
title	Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
title_short	Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
title_full	Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
title_fullStr	Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
title_full_unstemmed	Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
title_sort	cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (sbp): application to the purification of sbp from toad
publishDate	1987
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03784347_v415_nC_p297_SantaColoma http://hdl.handle.net/20.500.12110/paper_03784347_v415_nC_p297_SantaColoma
work_keys_str_mv	AT santacolomatomasantonio cyclicbiospecificaffinitychromatographicmethodforthepurificationofthesexsteroidbindingproteinsbpapplicationtothepurificationofsbpfromtoad AT garraffohugomartin cyclicbiospecificaffinitychromatographicmethodforthepurificationofthesexsteroidbindingproteinsbpapplicationtothepurificationofsbpfromtoad AT groseduardogervasio cyclicbiospecificaffinitychromatographicmethodforthepurificationofthesexsteroidbindingproteinsbpapplicationtothepurificationofsbpfromtoad AT charreaueduardohernan cyclicbiospecificaffinitychromatographicmethodforthepurificationofthesexsteroidbindingproteinsbpapplicationtothepurificationofsbpfromtoad
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Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad

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