Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats
The hypothalamic-growth hormone (GH)-liver axis represents a new concept in endocrine regulation of drug toxicity. Preponderant sex differences are found in liver gene expression, mostly dependent on the sexually dimorphic pattern of GH secretion which is set during the neonatal period by gonadal st...
Guardado en:
| Publicado: |
2012
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03784274_v213_n3_p325_Ramirez http://hdl.handle.net/20.500.12110/paper_03784274_v213_n3_p325_Ramirez |
| Aporte de: |
| id |
paper:paper_03784274_v213_n3_p325_Ramirez |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_03784274_v213_n3_p325_Ramirez2023-06-08T15:39:43Z Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats Bisphenol Cyps Growth hormone Liver Prolactin Sexual dimorphism 4,4' isopropylidenediphenol alcohol dehydrogenase castor oil complementary DNA cytochrome P450 2C11 cytochrome P450 2C12 endocrine disruptor glyceraldehyde 3 phosphate dehydrogenase growth hormone liver enzyme messenger RNA prolactin prolactin receptor RNA somatomedin C unclassified drug xenoestrogen animal experiment animal tissue article controlled study detoxification electrophoresis environmental exposure gene expression growth hormone release hormone blood level hypothalamus liver liver metabolism mouse newborn nonhuman perinatal period polyacrylamide gel electrophoresis priority journal protein urine level radioimmunoassay real time polymerase chain reaction Age Factors Aging Alcohol Dehydrogenase Animals Animals, Newborn Aryl Hydrocarbon Hydroxylases Drug Administration Schedule Electrophoresis, Polyacrylamide Gel Endocrine Disruptors Estrogens, Non-Steroidal Female Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Growth Hormone Hepatocyte Nuclear Factor 6 Injections, Subcutaneous Insulin-Like Growth Factor I Liver Male Phenols Pituitary Gland Prolactin Rats Rats, Sprague-Dawley Real-Time Polymerase Chain Reaction Receptors, Prolactin RNA, Messenger Sex Characteristics Sex Factors Steroid 16-alpha-Hydroxylase Steroid Hydroxylases Rattus The hypothalamic-growth hormone (GH)-liver axis represents a new concept in endocrine regulation of drug toxicity. Preponderant sex differences are found in liver gene expression, mostly dependent on the sexually dimorphic pattern of GH secretion which is set during the neonatal period by gonadal steroids. We tested if GH-dependent sexually dimorphic liver enzymes and proteins was perturbed by neonatal Bisphenol A (BPA) treatment in female rats. Female rats were sc injected with BPA (50 or 500μg/50μl) or castor oil vehicle from postnatal day 1 to 10. At five months serum prolactin, pituitary GH, and serum and liver insulin growth factor-I (IGF-I) were measured by RIA. Major urinary proteins (MUPs) were determined by electrophoresis. Liver Cyp2c11, Cyp2c12, Adh1, Hnf6, and Prlr mRNA levels were determined by real time PCR. Pituitary GH content and liver IGF-I concentration were increased by neonatal BPA treatment, indicating partial masculinization of the GH axis in treated females. GH-dependent female predominant liver enzyme genes (Cyp2c12 and Adh1) and a transcription factor (Hnf6) were downregulated or defeminized, while there were no changes in a male predominant gene (Cyp2c11) or protein (MUP). Our findings indicate that perinatal exposure to BPA may compromise the sexually dimorphic capacity of the liver to metabolize drugs and steroids. © 2012 Elsevier Ireland Ltd. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03784274_v213_n3_p325_Ramirez http://hdl.handle.net/20.500.12110/paper_03784274_v213_n3_p325_Ramirez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bisphenol Cyps Growth hormone Liver Prolactin Sexual dimorphism 4,4' isopropylidenediphenol alcohol dehydrogenase castor oil complementary DNA cytochrome P450 2C11 cytochrome P450 2C12 endocrine disruptor glyceraldehyde 3 phosphate dehydrogenase growth hormone liver enzyme messenger RNA prolactin prolactin receptor RNA somatomedin C unclassified drug xenoestrogen animal experiment animal tissue article controlled study detoxification electrophoresis environmental exposure gene expression growth hormone release hormone blood level hypothalamus liver liver metabolism mouse newborn nonhuman perinatal period polyacrylamide gel electrophoresis priority journal protein urine level radioimmunoassay real time polymerase chain reaction Age Factors Aging Alcohol Dehydrogenase Animals Animals, Newborn Aryl Hydrocarbon Hydroxylases Drug Administration Schedule Electrophoresis, Polyacrylamide Gel Endocrine Disruptors Estrogens, Non-Steroidal Female Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Growth Hormone Hepatocyte Nuclear Factor 6 Injections, Subcutaneous Insulin-Like Growth Factor I Liver Male Phenols Pituitary Gland Prolactin Rats Rats, Sprague-Dawley Real-Time Polymerase Chain Reaction Receptors, Prolactin RNA, Messenger Sex Characteristics Sex Factors Steroid 16-alpha-Hydroxylase Steroid Hydroxylases Rattus |
| spellingShingle |
Bisphenol Cyps Growth hormone Liver Prolactin Sexual dimorphism 4,4' isopropylidenediphenol alcohol dehydrogenase castor oil complementary DNA cytochrome P450 2C11 cytochrome P450 2C12 endocrine disruptor glyceraldehyde 3 phosphate dehydrogenase growth hormone liver enzyme messenger RNA prolactin prolactin receptor RNA somatomedin C unclassified drug xenoestrogen animal experiment animal tissue article controlled study detoxification electrophoresis environmental exposure gene expression growth hormone release hormone blood level hypothalamus liver liver metabolism mouse newborn nonhuman perinatal period polyacrylamide gel electrophoresis priority journal protein urine level radioimmunoassay real time polymerase chain reaction Age Factors Aging Alcohol Dehydrogenase Animals Animals, Newborn Aryl Hydrocarbon Hydroxylases Drug Administration Schedule Electrophoresis, Polyacrylamide Gel Endocrine Disruptors Estrogens, Non-Steroidal Female Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Growth Hormone Hepatocyte Nuclear Factor 6 Injections, Subcutaneous Insulin-Like Growth Factor I Liver Male Phenols Pituitary Gland Prolactin Rats Rats, Sprague-Dawley Real-Time Polymerase Chain Reaction Receptors, Prolactin RNA, Messenger Sex Characteristics Sex Factors Steroid 16-alpha-Hydroxylase Steroid Hydroxylases Rattus Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats |
| topic_facet |
Bisphenol Cyps Growth hormone Liver Prolactin Sexual dimorphism 4,4' isopropylidenediphenol alcohol dehydrogenase castor oil complementary DNA cytochrome P450 2C11 cytochrome P450 2C12 endocrine disruptor glyceraldehyde 3 phosphate dehydrogenase growth hormone liver enzyme messenger RNA prolactin prolactin receptor RNA somatomedin C unclassified drug xenoestrogen animal experiment animal tissue article controlled study detoxification electrophoresis environmental exposure gene expression growth hormone release hormone blood level hypothalamus liver liver metabolism mouse newborn nonhuman perinatal period polyacrylamide gel electrophoresis priority journal protein urine level radioimmunoassay real time polymerase chain reaction Age Factors Aging Alcohol Dehydrogenase Animals Animals, Newborn Aryl Hydrocarbon Hydroxylases Drug Administration Schedule Electrophoresis, Polyacrylamide Gel Endocrine Disruptors Estrogens, Non-Steroidal Female Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Growth Hormone Hepatocyte Nuclear Factor 6 Injections, Subcutaneous Insulin-Like Growth Factor I Liver Male Phenols Pituitary Gland Prolactin Rats Rats, Sprague-Dawley Real-Time Polymerase Chain Reaction Receptors, Prolactin RNA, Messenger Sex Characteristics Sex Factors Steroid 16-alpha-Hydroxylase Steroid Hydroxylases Rattus |
| description |
The hypothalamic-growth hormone (GH)-liver axis represents a new concept in endocrine regulation of drug toxicity. Preponderant sex differences are found in liver gene expression, mostly dependent on the sexually dimorphic pattern of GH secretion which is set during the neonatal period by gonadal steroids. We tested if GH-dependent sexually dimorphic liver enzymes and proteins was perturbed by neonatal Bisphenol A (BPA) treatment in female rats. Female rats were sc injected with BPA (50 or 500μg/50μl) or castor oil vehicle from postnatal day 1 to 10. At five months serum prolactin, pituitary GH, and serum and liver insulin growth factor-I (IGF-I) were measured by RIA. Major urinary proteins (MUPs) were determined by electrophoresis. Liver Cyp2c11, Cyp2c12, Adh1, Hnf6, and Prlr mRNA levels were determined by real time PCR. Pituitary GH content and liver IGF-I concentration were increased by neonatal BPA treatment, indicating partial masculinization of the GH axis in treated females. GH-dependent female predominant liver enzyme genes (Cyp2c12 and Adh1) and a transcription factor (Hnf6) were downregulated or defeminized, while there were no changes in a male predominant gene (Cyp2c11) or protein (MUP). Our findings indicate that perinatal exposure to BPA may compromise the sexually dimorphic capacity of the liver to metabolize drugs and steroids. © 2012 Elsevier Ireland Ltd. |
| title |
Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats |
| title_short |
Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats |
| title_full |
Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats |
| title_fullStr |
Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats |
| title_full_unstemmed |
Neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats |
| title_sort |
neonatal xenoestrogen exposure alters growth hormone-dependent liver proteins and genes in adult female rats |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03784274_v213_n3_p325_Ramirez http://hdl.handle.net/20.500.12110/paper_03784274_v213_n3_p325_Ramirez |
| _version_ |
1768545096358166528 |