DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives

Tinidazole (TNZ), ornidazole (ONZ) and metronidazole (MTZ) are antiparasitic drugs (nitroimidazole derivatives) that have proven to be effective against Trichomonas vaginalis, Entoamoeba histolytica, Giardia lamblia and Helicobacter pylori. The reduction of the nitro group and the generation of shor...

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Publicado:	2002
Materias:	Comet assay DNA damage Metronidazole Nitroimidazoles Ornidazole Tinidazole DNA metronidazole nitroimidazole derivative ornidazole reactive oxygen metabolite tinidazole aerobic metabolism antimicrobial activity article bacterial cell comet assay concentration response controlled study DNA strand breakage drug cytotoxicity human human cell lymphocyte mammal cell mutagenic activity priority journal Adult Antitrichomonal Agents Cells, Cultured Comet Assay DNA Damage DNA, Single-Stranded Dose-Response Relationship, Drug Humans Lymphocytes Male Giardia Giardia intestinalis Helicobacter Helicobacter pylori Mammalia Trichomonas vaginalis
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03784274_v132_n2_p109_RodriguezFerreiro http://hdl.handle.net/20.500.12110/paper_03784274_v132_n2_p109_RodriguezFerreiro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03784274_v132_n2_p109_RodriguezFerreiro
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spelling	paper:paper_03784274_v132_n2_p109_RodriguezFerreiro2023-06-08T15:39:43Z DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives Comet assay DNA damage Metronidazole Nitroimidazoles Ornidazole Tinidazole DNA metronidazole nitroimidazole derivative ornidazole reactive oxygen metabolite tinidazole aerobic metabolism antimicrobial activity article bacterial cell comet assay concentration response controlled study DNA damage DNA strand breakage drug cytotoxicity human human cell lymphocyte mammal cell mutagenic activity priority journal Adult Antitrichomonal Agents Cells, Cultured Comet Assay DNA Damage DNA, Single-Stranded Dose-Response Relationship, Drug Humans Lymphocytes Male Metronidazole Nitroimidazoles Ornidazole Tinidazole Giardia Giardia intestinalis Helicobacter Helicobacter pylori Mammalia Trichomonas vaginalis Tinidazole (TNZ), ornidazole (ONZ) and metronidazole (MTZ) are antiparasitic drugs (nitroimidazole derivatives) that have proven to be effective against Trichomonas vaginalis, Entoamoeba histolytica, Giardia lamblia and Helicobacter pylori. The reduction of the nitro group and the generation of short-lived reactive intermediates are the basis of its parasiticidal activity. This reduction is associated with its mutagenic activity in bacteria, although in mammalian cells DNA damage seems to be related to the production of reactive oxygen species (ROS). Using alkaline single cell electrophoresis, a significant increase in single strand breaks and alkali labile sites in human peripheral blood lymphocytes (PBL) exposed to MTZ, ONZ and TNZ at 10, 100 and 500 μg/ml is observed. MTZ causes less damage, especially at higher concentrations, when compared with TNZ, the most harmful of the drugs tested. These findings suggest that primary damage is induced under aerobic conditions and confirms that these nitroimidazoles are DNA damaging agents. © 2002 Elsevier Science Ireland Ltd. All rights reserved. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03784274_v132_n2_p109_RodriguezFerreiro http://hdl.handle.net/20.500.12110/paper_03784274_v132_n2_p109_RodriguezFerreiro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Comet assay DNA damage Metronidazole Nitroimidazoles Ornidazole Tinidazole DNA metronidazole nitroimidazole derivative ornidazole reactive oxygen metabolite tinidazole aerobic metabolism antimicrobial activity article bacterial cell comet assay concentration response controlled study DNA damage DNA strand breakage drug cytotoxicity human human cell lymphocyte mammal cell mutagenic activity priority journal Adult Antitrichomonal Agents Cells, Cultured Comet Assay DNA Damage DNA, Single-Stranded Dose-Response Relationship, Drug Humans Lymphocytes Male Metronidazole Nitroimidazoles Ornidazole Tinidazole Giardia Giardia intestinalis Helicobacter Helicobacter pylori Mammalia Trichomonas vaginalis
spellingShingle	Comet assay DNA damage Metronidazole Nitroimidazoles Ornidazole Tinidazole DNA metronidazole nitroimidazole derivative ornidazole reactive oxygen metabolite tinidazole aerobic metabolism antimicrobial activity article bacterial cell comet assay concentration response controlled study DNA damage DNA strand breakage drug cytotoxicity human human cell lymphocyte mammal cell mutagenic activity priority journal Adult Antitrichomonal Agents Cells, Cultured Comet Assay DNA Damage DNA, Single-Stranded Dose-Response Relationship, Drug Humans Lymphocytes Male Metronidazole Nitroimidazoles Ornidazole Tinidazole Giardia Giardia intestinalis Helicobacter Helicobacter pylori Mammalia Trichomonas vaginalis DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives
topic_facet	Comet assay DNA damage Metronidazole Nitroimidazoles Ornidazole Tinidazole DNA metronidazole nitroimidazole derivative ornidazole reactive oxygen metabolite tinidazole aerobic metabolism antimicrobial activity article bacterial cell comet assay concentration response controlled study DNA damage DNA strand breakage drug cytotoxicity human human cell lymphocyte mammal cell mutagenic activity priority journal Adult Antitrichomonal Agents Cells, Cultured Comet Assay DNA Damage DNA, Single-Stranded Dose-Response Relationship, Drug Humans Lymphocytes Male Metronidazole Nitroimidazoles Ornidazole Tinidazole Giardia Giardia intestinalis Helicobacter Helicobacter pylori Mammalia Trichomonas vaginalis
description	Tinidazole (TNZ), ornidazole (ONZ) and metronidazole (MTZ) are antiparasitic drugs (nitroimidazole derivatives) that have proven to be effective against Trichomonas vaginalis, Entoamoeba histolytica, Giardia lamblia and Helicobacter pylori. The reduction of the nitro group and the generation of short-lived reactive intermediates are the basis of its parasiticidal activity. This reduction is associated with its mutagenic activity in bacteria, although in mammalian cells DNA damage seems to be related to the production of reactive oxygen species (ROS). Using alkaline single cell electrophoresis, a significant increase in single strand breaks and alkali labile sites in human peripheral blood lymphocytes (PBL) exposed to MTZ, ONZ and TNZ at 10, 100 and 500 μg/ml is observed. MTZ causes less damage, especially at higher concentrations, when compared with TNZ, the most harmful of the drugs tested. These findings suggest that primary damage is induced under aerobic conditions and confirms that these nitroimidazoles are DNA damaging agents. © 2002 Elsevier Science Ireland Ltd. All rights reserved.
title	DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives
title_short	DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives
title_full	DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives
title_fullStr	DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives
title_full_unstemmed	DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives
title_sort	dna single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives
publishDate	2002
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03784274_v132_n2_p109_RodriguezFerreiro http://hdl.handle.net/20.500.12110/paper_03784274_v132_n2_p109_RodriguezFerreiro
_version_	1768544552937848832

DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives

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