Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi

Cell-free extracts of epimastigotes of Trypanosoma cruzi contain tyrosine aminotransferase (TAT) and p-hydroxyphenyllactate dehydrogenase (pHPLDH). The TAT activity could be separated from aspartate aminotransferase (ASAT) by polyacrylamide gel electrophoresis or DEAE-cellulose chromatography; the l...

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Publicado:	1992
Materias:	p-Hydroxyphenyl lactate dehydrogenase Trypanosoma cruzi Tyrosine aminotransferase 4 hydroxyphenyllactic acid 4-hydroxyphenyllactic acid oxidoreductase phenylpropionic acid derivative tyrosine aminotransferase aspartate aminotransferase animal article cell cycle cell fractionation enzymology isolation and purification metabolism ultrastructure epimastigote mitochondrion nonhuman priority journal protein localization trypanosoma cruzi Animal Cell Cycle Oxidoreductases Phenylpropionates Subcellular Fractions Support, Non-U.S. Gov't Tyrosine Transaminase Trypanosoma
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v92_n2_p119_Nowicki http://hdl.handle.net/20.500.12110/paper_03781097_v92_n2_p119_Nowicki
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03781097_v92_n2_p119_Nowicki
record_format	dspace
spelling	paper:paper_03781097_v92_n2_p119_Nowicki2023-06-08T15:39:13Z Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi p-Hydroxyphenyl lactate dehydrogenase Trypanosoma cruzi Tyrosine aminotransferase 4 hydroxyphenyllactic acid 4-hydroxyphenyllactic acid oxidoreductase phenylpropionic acid derivative tyrosine aminotransferase aspartate aminotransferase tyrosine aminotransferase animal article cell cycle cell fractionation enzymology isolation and purification metabolism Trypanosoma cruzi ultrastructure epimastigote mitochondrion nonhuman priority journal protein localization trypanosoma cruzi Animal Cell Cycle Oxidoreductases Phenylpropionates Subcellular Fractions Support, Non-U.S. Gov't Trypanosoma cruzi Tyrosine Transaminase Trypanosoma Trypanosoma cruzi Cell-free extracts of epimastigotes of Trypanosoma cruzi contain tyrosine aminotransferase (TAT) and p-hydroxyphenyllactate dehydrogenase (pHPLDH). The TAT activity could be separated from aspartate aminotransferase (ASAT) by polyacrylamide gel electrophoresis or DEAE-cellulose chromatography; the latter procedure also allowed complete separation of pHPLDH. The subcellular localization of both T. cruzi enzymes, as determined by digitonin extraction, subcellular fractionation by differential centrifugation, and isopycnic ultracentrifugation in sucrose gradients, was mainly cytosolic, with low mitochondrial activities. © 1992. 1992 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v92_n2_p119_Nowicki http://hdl.handle.net/20.500.12110/paper_03781097_v92_n2_p119_Nowicki
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	p-Hydroxyphenyl lactate dehydrogenase Trypanosoma cruzi Tyrosine aminotransferase 4 hydroxyphenyllactic acid 4-hydroxyphenyllactic acid oxidoreductase phenylpropionic acid derivative tyrosine aminotransferase aspartate aminotransferase tyrosine aminotransferase animal article cell cycle cell fractionation enzymology isolation and purification metabolism Trypanosoma cruzi ultrastructure epimastigote mitochondrion nonhuman priority journal protein localization trypanosoma cruzi Animal Cell Cycle Oxidoreductases Phenylpropionates Subcellular Fractions Support, Non-U.S. Gov't Trypanosoma cruzi Tyrosine Transaminase Trypanosoma Trypanosoma cruzi
spellingShingle	p-Hydroxyphenyl lactate dehydrogenase Trypanosoma cruzi Tyrosine aminotransferase 4 hydroxyphenyllactic acid 4-hydroxyphenyllactic acid oxidoreductase phenylpropionic acid derivative tyrosine aminotransferase aspartate aminotransferase tyrosine aminotransferase animal article cell cycle cell fractionation enzymology isolation and purification metabolism Trypanosoma cruzi ultrastructure epimastigote mitochondrion nonhuman priority journal protein localization trypanosoma cruzi Animal Cell Cycle Oxidoreductases Phenylpropionates Subcellular Fractions Support, Non-U.S. Gov't Trypanosoma cruzi Tyrosine Transaminase Trypanosoma Trypanosoma cruzi Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi
topic_facet	p-Hydroxyphenyl lactate dehydrogenase Trypanosoma cruzi Tyrosine aminotransferase 4 hydroxyphenyllactic acid 4-hydroxyphenyllactic acid oxidoreductase phenylpropionic acid derivative tyrosine aminotransferase aspartate aminotransferase tyrosine aminotransferase animal article cell cycle cell fractionation enzymology isolation and purification metabolism Trypanosoma cruzi ultrastructure epimastigote mitochondrion nonhuman priority journal protein localization trypanosoma cruzi Animal Cell Cycle Oxidoreductases Phenylpropionates Subcellular Fractions Support, Non-U.S. Gov't Trypanosoma cruzi Tyrosine Transaminase Trypanosoma Trypanosoma cruzi
description	Cell-free extracts of epimastigotes of Trypanosoma cruzi contain tyrosine aminotransferase (TAT) and p-hydroxyphenyllactate dehydrogenase (pHPLDH). The TAT activity could be separated from aspartate aminotransferase (ASAT) by polyacrylamide gel electrophoresis or DEAE-cellulose chromatography; the latter procedure also allowed complete separation of pHPLDH. The subcellular localization of both T. cruzi enzymes, as determined by digitonin extraction, subcellular fractionation by differential centrifugation, and isopycnic ultracentrifugation in sucrose gradients, was mainly cytosolic, with low mitochondrial activities. © 1992.
title	Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi
title_short	Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi
title_full	Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi
title_fullStr	Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi
title_full_unstemmed	Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi
title_sort	presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of trypanosoma cruzi
publishDate	1992
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v92_n2_p119_Nowicki http://hdl.handle.net/20.500.12110/paper_03781097_v92_n2_p119_Nowicki
_version_	1768546725886164992

Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi

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