A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli

Plasmid pPG1 from Staphylococcus aureus coding for ampicillin (Apr), gentamicin (Gmr) and amikacin (Akr) resistance was transformed into Escherichia coli. Transformation efficiency was about 2 × 103 transformants/μg of plasmid DNA. The plasmids present in the E. coli transformants were identical to...

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Autor principal:	Gadaleta, Patricia Gladys
Publicado:	1991
Materias:	Antibiotic resistance Hetero specific gene exchange Staphylococcus plasmid amikacin gentamicin ampicillin chloramphenicol tetracycline antibiotic resistance article bacterium transformation Escherichia coli genetics penicillin resistance plasmid Staphylococcus aureus dna hybridization escherichia coli nonhuman priority journal southern blotting staphylococcus Amikacin Ampicillin Resistance Drug Resistance, Microbial Gentamicins Plasmids Support, Non-U.S. Gov't Transformation, Bacterial Staphylococcus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v80_n2-3_p147_Gadaleta http://hdl.handle.net/20.500.12110/paper_03781097_v80_n2-3_p147_Gadaleta
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03781097_v80_n2-3_p147_Gadaleta
record_format	dspace
spelling	paper:paper_03781097_v80_n2-3_p147_Gadaleta2023-06-08T15:39:12Z A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli Gadaleta, Patricia Gladys Antibiotic resistance Hetero specific gene exchange Staphylococcus plasmid amikacin gentamicin amikacin ampicillin chloramphenicol gentamicin tetracycline antibiotic resistance article bacterium transformation Escherichia coli genetics penicillin resistance plasmid Staphylococcus aureus dna hybridization escherichia coli nonhuman plasmid priority journal southern blotting staphylococcus Amikacin Ampicillin Resistance Drug Resistance, Microbial Escherichia coli Gentamicins Plasmids Staphylococcus aureus Support, Non-U.S. Gov't Transformation, Bacterial Escherichia coli Staphylococcus Staphylococcus aureus Plasmid pPG1 from Staphylococcus aureus coding for ampicillin (Apr), gentamicin (Gmr) and amikacin (Akr) resistance was transformed into Escherichia coli. Transformation efficiency was about 2 × 103 transformants/μg of plasmid DNA. The plasmids present in the E. coli transformants were identical to pPG1 according to their restriction patterns. The copy number of pPG1 was estimated to be at least 20-times less in E. coli than in S. aureus. The minimal inhibitory concentrations (MICs) for Ap and Gm were lower in E. coli than in S. aureus. However, the MIC for Ak was higher in E. coli transformants than in S. aureus. pPG1 was maintained in the E. coli transformants for at least 80 generations at 37°C without antibiotic selection pressure. © 1991. Fil:Gadaleta, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1991 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v80_n2-3_p147_Gadaleta http://hdl.handle.net/20.500.12110/paper_03781097_v80_n2-3_p147_Gadaleta
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antibiotic resistance Hetero specific gene exchange Staphylococcus plasmid amikacin gentamicin amikacin ampicillin chloramphenicol gentamicin tetracycline antibiotic resistance article bacterium transformation Escherichia coli genetics penicillin resistance plasmid Staphylococcus aureus dna hybridization escherichia coli nonhuman plasmid priority journal southern blotting staphylococcus Amikacin Ampicillin Resistance Drug Resistance, Microbial Escherichia coli Gentamicins Plasmids Staphylococcus aureus Support, Non-U.S. Gov't Transformation, Bacterial Escherichia coli Staphylococcus Staphylococcus aureus
spellingShingle	Antibiotic resistance Hetero specific gene exchange Staphylococcus plasmid amikacin gentamicin amikacin ampicillin chloramphenicol gentamicin tetracycline antibiotic resistance article bacterium transformation Escherichia coli genetics penicillin resistance plasmid Staphylococcus aureus dna hybridization escherichia coli nonhuman plasmid priority journal southern blotting staphylococcus Amikacin Ampicillin Resistance Drug Resistance, Microbial Escherichia coli Gentamicins Plasmids Staphylococcus aureus Support, Non-U.S. Gov't Transformation, Bacterial Escherichia coli Staphylococcus Staphylococcus aureus Gadaleta, Patricia Gladys A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli
topic_facet	Antibiotic resistance Hetero specific gene exchange Staphylococcus plasmid amikacin gentamicin amikacin ampicillin chloramphenicol gentamicin tetracycline antibiotic resistance article bacterium transformation Escherichia coli genetics penicillin resistance plasmid Staphylococcus aureus dna hybridization escherichia coli nonhuman plasmid priority journal southern blotting staphylococcus Amikacin Ampicillin Resistance Drug Resistance, Microbial Escherichia coli Gentamicins Plasmids Staphylococcus aureus Support, Non-U.S. Gov't Transformation, Bacterial Escherichia coli Staphylococcus Staphylococcus aureus
description	Plasmid pPG1 from Staphylococcus aureus coding for ampicillin (Apr), gentamicin (Gmr) and amikacin (Akr) resistance was transformed into Escherichia coli. Transformation efficiency was about 2 × 103 transformants/μg of plasmid DNA. The plasmids present in the E. coli transformants were identical to pPG1 according to their restriction patterns. The copy number of pPG1 was estimated to be at least 20-times less in E. coli than in S. aureus. The minimal inhibitory concentrations (MICs) for Ap and Gm were lower in E. coli than in S. aureus. However, the MIC for Ak was higher in E. coli transformants than in S. aureus. pPG1 was maintained in the E. coli transformants for at least 80 generations at 37°C without antibiotic selection pressure. © 1991.
author	Gadaleta, Patricia Gladys
author_facet	Gadaleta, Patricia Gladys
author_sort	Gadaleta, Patricia Gladys
title	A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli
title_short	A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli
title_full	A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli
title_fullStr	A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli
title_full_unstemmed	A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli
title_sort	staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in escherichia coli
publishDate	1991
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v80_n2-3_p147_Gadaleta http://hdl.handle.net/20.500.12110/paper_03781097_v80_n2-3_p147_Gadaleta
work_keys_str_mv	AT gadaletapatriciagladys astaphylococcalplasmidthatreplicatesandexpressesampicillingentamicinandamikacinresistanceinescherichiacoli AT gadaletapatriciagladys staphylococcalplasmidthatreplicatesandexpressesampicillingentamicinandamikacinresistanceinescherichiacoli
_version_	1768541897029058560

A staphylococcal plasmid that replicates and expresses ampicillin, gentamicin and amikacin resistance in Escherichia coli

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