Digestion of human immunoglobulin G by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi
The major cysteine proteinase (cruzipain) from Trypanosoma cruzi was able to digest human IgG, as shown by polyacrylamide gel electrophoresis in the presence of SDS, and by gel filtration on a Superose 12 column, in a FPLC system. The Fab fragment of IgG was only slightly degraded, but Fc was extens...
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1990
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v70_n3_p337_Bontempi http://hdl.handle.net/20.500.12110/paper_03781097_v70_n3_p337_Bontempi |
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paper:paper_03781097_v70_n3_p337_Bontempi2023-06-08T15:39:12Z Digestion of human immunoglobulin G by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi Bontempi, Esteban José Crazipain Cysteine proteinase Fc degradation IgG degradation Trypanosoma cruzi proteinase cruzipain cysteine proteinase immunoglobulin F(ab) fragment immunoglobulin Fc fragment immunoglobulin G article human human cell nonhuman priority journal protozoon trypanosoma cruzi animal enzymology hydrolysis Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi Animal Cysteine Endopeptidases Human Hydrolysis Immunoglobulin G Immunoglobulins, Fab Immunoglobulins, Fc Support, Non-U.S. Gov't Trypanosoma cruzi The major cysteine proteinase (cruzipain) from Trypanosoma cruzi was able to digest human IgG, as shown by polyacrylamide gel electrophoresis in the presence of SDS, and by gel filtration on a Superose 12 column, in a FPLC system. The Fab fragment of IgG was only slightly degraded, but Fc was extensively hydrolyzed to small peptides. The results suggest that cruzipain might be involved in the defense mechanisms of the parasite against the immune response of the host. © 1990 Federation of European Microbiological Societies. Fil:Bontempi, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v70_n3_p337_Bontempi http://hdl.handle.net/20.500.12110/paper_03781097_v70_n3_p337_Bontempi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Crazipain Cysteine proteinase Fc degradation IgG degradation Trypanosoma cruzi proteinase cruzipain cysteine proteinase immunoglobulin F(ab) fragment immunoglobulin Fc fragment immunoglobulin G article human human cell nonhuman priority journal protozoon trypanosoma cruzi animal enzymology hydrolysis Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi Animal Cysteine Endopeptidases Human Hydrolysis Immunoglobulin G Immunoglobulins, Fab Immunoglobulins, Fc Support, Non-U.S. Gov't Trypanosoma cruzi |
| spellingShingle |
Crazipain Cysteine proteinase Fc degradation IgG degradation Trypanosoma cruzi proteinase cruzipain cysteine proteinase immunoglobulin F(ab) fragment immunoglobulin Fc fragment immunoglobulin G article human human cell nonhuman priority journal protozoon trypanosoma cruzi animal enzymology hydrolysis Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi Animal Cysteine Endopeptidases Human Hydrolysis Immunoglobulin G Immunoglobulins, Fab Immunoglobulins, Fc Support, Non-U.S. Gov't Trypanosoma cruzi Bontempi, Esteban José Digestion of human immunoglobulin G by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi |
| topic_facet |
Crazipain Cysteine proteinase Fc degradation IgG degradation Trypanosoma cruzi proteinase cruzipain cysteine proteinase immunoglobulin F(ab) fragment immunoglobulin Fc fragment immunoglobulin G article human human cell nonhuman priority journal protozoon trypanosoma cruzi animal enzymology hydrolysis Trypanosoma cruzi Protozoa Trypanosoma Trypanosoma cruzi Animal Cysteine Endopeptidases Human Hydrolysis Immunoglobulin G Immunoglobulins, Fab Immunoglobulins, Fc Support, Non-U.S. Gov't Trypanosoma cruzi |
| description |
The major cysteine proteinase (cruzipain) from Trypanosoma cruzi was able to digest human IgG, as shown by polyacrylamide gel electrophoresis in the presence of SDS, and by gel filtration on a Superose 12 column, in a FPLC system. The Fab fragment of IgG was only slightly degraded, but Fc was extensively hydrolyzed to small peptides. The results suggest that cruzipain might be involved in the defense mechanisms of the parasite against the immune response of the host. © 1990 Federation of European Microbiological Societies. |
| author |
Bontempi, Esteban José |
| author_facet |
Bontempi, Esteban José |
| author_sort |
Bontempi, Esteban José |
| title |
Digestion of human immunoglobulin G by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi |
| title_short |
Digestion of human immunoglobulin G by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi |
| title_full |
Digestion of human immunoglobulin G by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi |
| title_fullStr |
Digestion of human immunoglobulin G by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi |
| title_full_unstemmed |
Digestion of human immunoglobulin G by the major cysteine proteinase (cruzipain) from Trypanosoma cruzi |
| title_sort |
digestion of human immunoglobulin g by the major cysteine proteinase (cruzipain) from trypanosoma cruzi |
| publishDate |
1990 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v70_n3_p337_Bontempi http://hdl.handle.net/20.500.12110/paper_03781097_v70_n3_p337_Bontempi |
| work_keys_str_mv |
AT bontempiestebanjose digestionofhumanimmunoglobulingbythemajorcysteineproteinasecruzipainfromtrypanosomacruzi |
| _version_ |
1768546254996897792 |