Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum
Little is known about isoprenoid biosynthesis in parasitic protozoa. The presence of dolichol and isoprenylated proteins has been detected in Plasmodium falciparum, but no studies are available about the biosynthesis of the isoprenic side chain attached to the benzoquinone ring of coenzyme Q. In the...
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2002
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v207_n1_p13_DeMacedo http://hdl.handle.net/20.500.12110/paper_03781097_v207_n1_p13_DeMacedo |
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paper:paper_03781097_v207_n1_p13_DeMacedo2023-06-08T15:39:08Z Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum Apicomplexan Coenzyme Q Isoprenoid Malaria Plasmodium falciparum isoprenoid nerolidol ubiquinone antimalarial activity article biosynthesis controlled study drug mechanism erythrocyte IC 50 nonhuman parasite development Plasmodium falciparum priority journal Animals Carbon Radioisotopes Erythrocytes Humans Malaria, Falciparum Parasitic Sensitivity Tests Plasmodium falciparum Polyisoprenyl Phosphates Protein Isoprenylation Sesquiterpenes Tritium Ubiquinone Plasmodium falciparum Protozoa Little is known about isoprenoid biosynthesis in parasitic protozoa. The presence of dolichol and isoprenylated proteins has been detected in Plasmodium falciparum, but no studies are available about the biosynthesis of the isoprenic side chain attached to the benzoquinone ring of coenzyme Q. In the present study, using metabolic labelling with different intermediates, we demonstrated the presence of an active isoprenoid pathway for the biosynthesis of the isoprenic chain of coenzyme Q. Our results show that P. falciparum is able to synthesize different homologs (coenzyme Q8 and coenzyme Q9), depending on the given intermediate. Parasites treated with nerolidol at doses 2.2 times below the IC50 showed a decreased ability to synthesize the isoprenic chain attached to coenzyme Q at all intraerythrocytic stages. Treatment with nerolidol arrested development of the intraerythrocytic stages of the parasites, indicating that the drug may have an antimalarial potential. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v207_n1_p13_DeMacedo http://hdl.handle.net/20.500.12110/paper_03781097_v207_n1_p13_DeMacedo |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Apicomplexan Coenzyme Q Isoprenoid Malaria Plasmodium falciparum isoprenoid nerolidol ubiquinone antimalarial activity article biosynthesis controlled study drug mechanism erythrocyte IC 50 nonhuman parasite development Plasmodium falciparum priority journal Animals Carbon Radioisotopes Erythrocytes Humans Malaria, Falciparum Parasitic Sensitivity Tests Plasmodium falciparum Polyisoprenyl Phosphates Protein Isoprenylation Sesquiterpenes Tritium Ubiquinone Plasmodium falciparum Protozoa |
spellingShingle |
Apicomplexan Coenzyme Q Isoprenoid Malaria Plasmodium falciparum isoprenoid nerolidol ubiquinone antimalarial activity article biosynthesis controlled study drug mechanism erythrocyte IC 50 nonhuman parasite development Plasmodium falciparum priority journal Animals Carbon Radioisotopes Erythrocytes Humans Malaria, Falciparum Parasitic Sensitivity Tests Plasmodium falciparum Polyisoprenyl Phosphates Protein Isoprenylation Sesquiterpenes Tritium Ubiquinone Plasmodium falciparum Protozoa Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum |
topic_facet |
Apicomplexan Coenzyme Q Isoprenoid Malaria Plasmodium falciparum isoprenoid nerolidol ubiquinone antimalarial activity article biosynthesis controlled study drug mechanism erythrocyte IC 50 nonhuman parasite development Plasmodium falciparum priority journal Animals Carbon Radioisotopes Erythrocytes Humans Malaria, Falciparum Parasitic Sensitivity Tests Plasmodium falciparum Polyisoprenyl Phosphates Protein Isoprenylation Sesquiterpenes Tritium Ubiquinone Plasmodium falciparum Protozoa |
description |
Little is known about isoprenoid biosynthesis in parasitic protozoa. The presence of dolichol and isoprenylated proteins has been detected in Plasmodium falciparum, but no studies are available about the biosynthesis of the isoprenic side chain attached to the benzoquinone ring of coenzyme Q. In the present study, using metabolic labelling with different intermediates, we demonstrated the presence of an active isoprenoid pathway for the biosynthesis of the isoprenic chain of coenzyme Q. Our results show that P. falciparum is able to synthesize different homologs (coenzyme Q8 and coenzyme Q9), depending on the given intermediate. Parasites treated with nerolidol at doses 2.2 times below the IC50 showed a decreased ability to synthesize the isoprenic chain attached to coenzyme Q at all intraerythrocytic stages. Treatment with nerolidol arrested development of the intraerythrocytic stages of the parasites, indicating that the drug may have an antimalarial potential. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. |
title |
Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum |
title_short |
Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum |
title_full |
Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum |
title_fullStr |
Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum |
title_full_unstemmed |
Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum |
title_sort |
characterization of the isoprenoid chain of coenzyme q in plasmodium falciparum |
publishDate |
2002 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v207_n1_p13_DeMacedo http://hdl.handle.net/20.500.12110/paper_03781097_v207_n1_p13_DeMacedo |
_version_ |
1768546025625092096 |