A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities

Tyrosine aminotransferase purified from epimastigotes of Trypanosoma cruzi displays an additional activity of alanine aminotransferase, absent in all other tyrosine aminotransferases characterized so far. Since the parasite's genome contains a high number of copies of the tyrosine aminotransfer...

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Detalles Bibliográficos
Autores principales:	Búa, Jacqueline Elena, Bontempi, Esteban José, Ruiz, Andrés Mariano
Publicado:	1995
Materias:	Alanine aminotransferase Fusion protein Recombinant transaminase Trypanosoma cruzi Tyrosine aminotransferase alanine aminotransferase hybrid protein tyrosine aminotransferase article controlled study enzyme activity molecular cloning nonhuman priority journal trypanosoma cruzi Alanine Transaminase Amino Acid Sequence Animal Blotting, Western Escherichia coli Gene Expression Regulation, Enzymologic Glutathione Transferase Molecular Sequence Data Recombinant Proteins Support, Non-U.S. Gov't Tyrosine Transaminase
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v133_n1-2_p17_Montemartini http://hdl.handle.net/20.500.12110/paper_03781097_v133_n1-2_p17_Montemartini
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03781097_v133_n1-2_p17_Montemartini
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spelling	paper:paper_03781097_v133_n1-2_p17_Montemartini2023-06-08T15:39:06Z A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities Búa, Jacqueline Elena Bontempi, Esteban José Ruiz, Andrés Mariano Alanine aminotransferase Fusion protein Recombinant transaminase Trypanosoma cruzi Tyrosine aminotransferase alanine aminotransferase hybrid protein tyrosine aminotransferase article controlled study enzyme activity molecular cloning nonhuman priority journal trypanosoma cruzi Alanine Transaminase Amino Acid Sequence Animal Blotting, Western Escherichia coli Gene Expression Regulation, Enzymologic Glutathione Transferase Molecular Sequence Data Recombinant Proteins Support, Non-U.S. Gov't Trypanosoma cruzi Tyrosine Transaminase Tyrosine aminotransferase purified from epimastigotes of Trypanosoma cruzi displays an additional activity of alanine aminotransferase, absent in all other tyrosine aminotransferases characterized so far. Since the parasite's genome contains a high number of copies of the tyrosine aminotransferase gene, we could not rule out the possibility that two very similar proteins, with changed specificity due to a few amino acid substitutions, might be responsible for the two activities. We have now expressed in Escherichia coli a recombinant tyrosine aminotransferase as a fusion protein with glutathione S-trans-ferase. The purified fusion protein, intact or after thrombin cleavage, displays tyrosine aminotransferase and alanine aminotransferase activities with apparent Km values similar to those for the natural enzyme, thus proving that they belong to the same protein. © 1995. Fil:Búa, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bontempi, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruiz, a.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v133_n1-2_p17_Montemartini http://hdl.handle.net/20.500.12110/paper_03781097_v133_n1-2_p17_Montemartini
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Alanine aminotransferase Fusion protein Recombinant transaminase Trypanosoma cruzi Tyrosine aminotransferase alanine aminotransferase hybrid protein tyrosine aminotransferase article controlled study enzyme activity molecular cloning nonhuman priority journal trypanosoma cruzi Alanine Transaminase Amino Acid Sequence Animal Blotting, Western Escherichia coli Gene Expression Regulation, Enzymologic Glutathione Transferase Molecular Sequence Data Recombinant Proteins Support, Non-U.S. Gov't Trypanosoma cruzi Tyrosine Transaminase
spellingShingle	Alanine aminotransferase Fusion protein Recombinant transaminase Trypanosoma cruzi Tyrosine aminotransferase alanine aminotransferase hybrid protein tyrosine aminotransferase article controlled study enzyme activity molecular cloning nonhuman priority journal trypanosoma cruzi Alanine Transaminase Amino Acid Sequence Animal Blotting, Western Escherichia coli Gene Expression Regulation, Enzymologic Glutathione Transferase Molecular Sequence Data Recombinant Proteins Support, Non-U.S. Gov't Trypanosoma cruzi Tyrosine Transaminase Búa, Jacqueline Elena Bontempi, Esteban José Ruiz, Andrés Mariano A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities
topic_facet	Alanine aminotransferase Fusion protein Recombinant transaminase Trypanosoma cruzi Tyrosine aminotransferase alanine aminotransferase hybrid protein tyrosine aminotransferase article controlled study enzyme activity molecular cloning nonhuman priority journal trypanosoma cruzi Alanine Transaminase Amino Acid Sequence Animal Blotting, Western Escherichia coli Gene Expression Regulation, Enzymologic Glutathione Transferase Molecular Sequence Data Recombinant Proteins Support, Non-U.S. Gov't Trypanosoma cruzi Tyrosine Transaminase
description	Tyrosine aminotransferase purified from epimastigotes of Trypanosoma cruzi displays an additional activity of alanine aminotransferase, absent in all other tyrosine aminotransferases characterized so far. Since the parasite's genome contains a high number of copies of the tyrosine aminotransferase gene, we could not rule out the possibility that two very similar proteins, with changed specificity due to a few amino acid substitutions, might be responsible for the two activities. We have now expressed in Escherichia coli a recombinant tyrosine aminotransferase as a fusion protein with glutathione S-trans-ferase. The purified fusion protein, intact or after thrombin cleavage, displays tyrosine aminotransferase and alanine aminotransferase activities with apparent Km values similar to those for the natural enzyme, thus proving that they belong to the same protein. © 1995.
author	Búa, Jacqueline Elena Bontempi, Esteban José Ruiz, Andrés Mariano
author_facet	Búa, Jacqueline Elena Bontempi, Esteban José Ruiz, Andrés Mariano
author_sort	Búa, Jacqueline Elena
title	A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities
title_short	A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities
title_full	A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities
title_fullStr	A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities
title_full_unstemmed	A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities
title_sort	recombinant tyrosine aminotransferase from trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities
publishDate	1995
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03781097_v133_n1-2_p17_Montemartini http://hdl.handle.net/20.500.12110/paper_03781097_v133_n1-2_p17_Montemartini
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A recombinant tyrosine aminotransferase from Trypanosoma cruzi has both tyrosine aminotransferase and alanine aminotransferase activities

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