Diagnostico de la hipertermia maligna en cerdos por PCR/REA

The malignant hyperthermia (MH) is an autosomal recessive illness with incomplete penetrance which produces major economic losses in pig production. The affected animals often have a sudden death and their meats can be confiscated due to its poor quality. The halothane test has been the method to cl...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Flawiá, Mirtha María, Torres, Héctor Norberto
Publicado: 1998
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v86_n3-6_p188_Soria
http://hdl.handle.net/20.500.12110/paper_03650375_v86_n3-6_p188_Soria
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03650375_v86_n3-6_p188_Soria
record_format dspace
spelling paper:paper_03650375_v86_n3-6_p188_Soria2023-06-08T15:35:47Z Diagnostico de la hipertermia maligna en cerdos por PCR/REA Flawiá, Mirtha María Torres, Héctor Norberto The malignant hyperthermia (MH) is an autosomal recessive illness with incomplete penetrance which produces major economic losses in pig production. The affected animals often have a sudden death and their meats can be confiscated due to its poor quality. The halothane test has been the method to classify pigs into affected and non affected animals. This indirect test does not distinguish resistant homozygous animals from heterozygous carriers. Furthermore, it is imprecise because some pigs do not react as expected from their genotype. A point mutation (C for T) in the nucleotide 1843 of the cDNA from the ryanodine receptor gene accounts for the MH etiology. Therefore, a molecular test provides a more accurate method to determine genotypes. Thirty-five pigs from EEA-Inta Pergamino have been analysed by PCR/REA in order to discuss their genotypes for the ryr-1 gene. The results from the original halothane test and breedings were compared with those obtained from PCR/REA: we found 4 animals that did not present the expected genotype. Fil:Flawia, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v86_n3-6_p188_Soria http://hdl.handle.net/20.500.12110/paper_03650375_v86_n3-6_p188_Soria
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description The malignant hyperthermia (MH) is an autosomal recessive illness with incomplete penetrance which produces major economic losses in pig production. The affected animals often have a sudden death and their meats can be confiscated due to its poor quality. The halothane test has been the method to classify pigs into affected and non affected animals. This indirect test does not distinguish resistant homozygous animals from heterozygous carriers. Furthermore, it is imprecise because some pigs do not react as expected from their genotype. A point mutation (C for T) in the nucleotide 1843 of the cDNA from the ryanodine receptor gene accounts for the MH etiology. Therefore, a molecular test provides a more accurate method to determine genotypes. Thirty-five pigs from EEA-Inta Pergamino have been analysed by PCR/REA in order to discuss their genotypes for the ryr-1 gene. The results from the original halothane test and breedings were compared with those obtained from PCR/REA: we found 4 animals that did not present the expected genotype.
author Flawiá, Mirtha María
Torres, Héctor Norberto
spellingShingle Flawiá, Mirtha María
Torres, Héctor Norberto
Diagnostico de la hipertermia maligna en cerdos por PCR/REA
author_facet Flawiá, Mirtha María
Torres, Héctor Norberto
author_sort Flawiá, Mirtha María
title Diagnostico de la hipertermia maligna en cerdos por PCR/REA
title_short Diagnostico de la hipertermia maligna en cerdos por PCR/REA
title_full Diagnostico de la hipertermia maligna en cerdos por PCR/REA
title_fullStr Diagnostico de la hipertermia maligna en cerdos por PCR/REA
title_full_unstemmed Diagnostico de la hipertermia maligna en cerdos por PCR/REA
title_sort diagnostico de la hipertermia maligna en cerdos por pcr/rea
publishDate 1998
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v86_n3-6_p188_Soria
http://hdl.handle.net/20.500.12110/paper_03650375_v86_n3-6_p188_Soria
work_keys_str_mv AT flawiamirthamaria diagnosticodelahipertermiamalignaencerdosporpcrrea
AT torreshectornorberto diagnosticodelahipertermiamalignaencerdosporpcrrea
_version_ 1768544914465882112

Ejemplares similares