Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS

Bile acids, bile salts and aminoacids are important tracers of different newborn pathologies. Their detection and quantification by simple and secure methods are very important, specially if they can be carried out on the original biological fluid, avoiding previous isolation. In this sense, tandem...

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Publicado: 1998
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v86_n3-6_p110_Seldes
http://hdl.handle.net/20.500.12110/paper_03650375_v86_n3-6_p110_Seldes
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03650375_v86_n3-6_p110_Seldes
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spelling paper:paper_03650375_v86_n3-6_p110_Seldes2023-06-08T15:35:45Z Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS Bile acids, bile salts and aminoacids are important tracers of different newborn pathologies. Their detection and quantification by simple and secure methods are very important, specially if they can be carried out on the original biological fluid, avoiding previous isolation. In this sense, tandem mass spectrometry is the method of choice. In order to improve the level of detection of these type of compounds when using negative FAB-MS-MS we tried different concentrations of each of them in four different matrixes: triethanolamine, glycerol, glycerol + NH4OH [1:2] and glycerol + NH4OH [2:1]. The results allowed the construction of the sensitivity curves and the calculation of the respective detection limits (LOD and LOQ) for each of the samples in each of the different matrixes. The use of glycerol + NH4OH [1:2] improved greatly the detection level for all of them and resulted in a simple and effective option for the proposed study. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v86_n3-6_p110_Seldes http://hdl.handle.net/20.500.12110/paper_03650375_v86_n3-6_p110_Seldes
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description Bile acids, bile salts and aminoacids are important tracers of different newborn pathologies. Their detection and quantification by simple and secure methods are very important, specially if they can be carried out on the original biological fluid, avoiding previous isolation. In this sense, tandem mass spectrometry is the method of choice. In order to improve the level of detection of these type of compounds when using negative FAB-MS-MS we tried different concentrations of each of them in four different matrixes: triethanolamine, glycerol, glycerol + NH4OH [1:2] and glycerol + NH4OH [2:1]. The results allowed the construction of the sensitivity curves and the calculation of the respective detection limits (LOD and LOQ) for each of the samples in each of the different matrixes. The use of glycerol + NH4OH [1:2] improved greatly the detection level for all of them and resulted in a simple and effective option for the proposed study.
title Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS
spellingShingle Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS
title_short Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS
title_full Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS
title_fullStr Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS
title_full_unstemmed Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS
title_sort improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative fab-ms-ms
publishDate 1998
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03650375_v86_n3-6_p110_Seldes
http://hdl.handle.net/20.500.12110/paper_03650375_v86_n3-6_p110_Seldes
_version_ 1768544868466950144

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