The 21-aminosteroid U-74389F attenuates hyperexpression of GAP-43 and NADPH-diaphorase in the spinal cord of wobbler mouse, a model for amyotrophic lateral sclerosis
The wobbler mouse suffers an autosomal recessive mutation producing severe neurodegeneration and astrogliosis in spinal cord. It has been considered a model for amyotrophic lateral sclerosis. We have studied in these animals the expression of two proteins, the growth-associated protein (GAP-43) and...
Guardado en:
| Publicado: |
1999
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v24_n1_p1_GonzalezDeniselle http://hdl.handle.net/20.500.12110/paper_03643190_v24_n1_p1_GonzalezDeniselle |
| Aporte de: |
| id |
paper:paper_03643190_v24_n1_p1_GonzalezDeniselle |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_03643190_v24_n1_p1_GonzalezDeniselle2023-06-08T15:35:34Z The 21-aminosteroid U-74389F attenuates hyperexpression of GAP-43 and NADPH-diaphorase in the spinal cord of wobbler mouse, a model for amyotrophic lateral sclerosis 21-aminosteroids GAP-43 NADPH-diaphorase Neurogeneration Wobbler mouse 21 [4 [2,6 bis(1 pyrrolidinyl) 4 pyrimidinyl] 1 piperazinyl]pregna 1,4,9(11) triene 3,20 dione 21 aminosteroid neuromodulin reduced nicotinamide adenine dinucleotide phosphate dehydrogenase amyotrophic lateral sclerosis animal experiment animal model article controlled study drug effect enzyme inhibition glia cell immunocytochemistry motoneuron mouse nerve fiber regeneration nerve sprouting nonhuman oxidative stress priority journal protein expression spinal cord Amyotrophic Lateral Sclerosis Animals Antioxidants Dihydrolipoamide Dehydrogenase Disease Models, Animal Female GAP-43 Protein Gene Expression Regulation Humans Male Mice Mice, Neurologic Mutants Motor Neurons Pregnatrienes Spinal Cord Animalia The wobbler mouse suffers an autosomal recessive mutation producing severe neurodegeneration and astrogliosis in spinal cord. It has been considered a model for amyotrophic lateral sclerosis. We have studied in these animals the expression of two proteins, the growth-associated protein (GAP-43) and the NADPH-diaphorase, the nitric oxide synthesizing enzyme, employing immunocytochemistry and histochemistry. We found higher expression of GAP-43 immunoreactivity in dorsal horn, Lamina X, corticospinal tract and ventral horn motoneurons in wobbler mice compared to controls. We NADPH- diaphorase activity was present in control motoneurons, in contrast to intense labeling of the wobbler group. No differences in diaphorase activity was measured in the rest of the spinal cord between control and mutant mice. A group of animals received subcutaneously for 4 days a 50 mg pellet of U- 74389F, a glucocorticoid-derived 21-aminosteroid with antioxidant properties but without glucocorticoid activity. U-74389F slightly attenuated GAP-43 immunostaining in dorsal regions of the spinal cord from wobblers but not in controls. However, in motoneurons of wobbler mice number of GAP-43 immunopositive neurons, cell processes and reaction intensity were reduced by U-74389F. The aminosteroid reduced by 50% motoneuron NADPH-diaphorase activity. Hyperexpression of GAP-43 immunoreactivity in wobbler mice may represent an exaggerated neuronal response to advancing degeneration or muscle denervation. It may also be linked to increased nitric oxide levels. U-74389F may stop neurodegeneration and/or increase muscle trophism and stop oxidative stress, consequently GAP-43 hyperexpression was attenuated. Wobbler mice may be important models to evaluate the use of antioxidant steroid therapy with a view to its use in human motoneuron disease. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v24_n1_p1_GonzalezDeniselle http://hdl.handle.net/20.500.12110/paper_03643190_v24_n1_p1_GonzalezDeniselle |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
21-aminosteroids GAP-43 NADPH-diaphorase Neurogeneration Wobbler mouse 21 [4 [2,6 bis(1 pyrrolidinyl) 4 pyrimidinyl] 1 piperazinyl]pregna 1,4,9(11) triene 3,20 dione 21 aminosteroid neuromodulin reduced nicotinamide adenine dinucleotide phosphate dehydrogenase amyotrophic lateral sclerosis animal experiment animal model article controlled study drug effect enzyme inhibition glia cell immunocytochemistry motoneuron mouse nerve fiber regeneration nerve sprouting nonhuman oxidative stress priority journal protein expression spinal cord Amyotrophic Lateral Sclerosis Animals Antioxidants Dihydrolipoamide Dehydrogenase Disease Models, Animal Female GAP-43 Protein Gene Expression Regulation Humans Male Mice Mice, Neurologic Mutants Motor Neurons Pregnatrienes Spinal Cord Animalia |
| spellingShingle |
21-aminosteroids GAP-43 NADPH-diaphorase Neurogeneration Wobbler mouse 21 [4 [2,6 bis(1 pyrrolidinyl) 4 pyrimidinyl] 1 piperazinyl]pregna 1,4,9(11) triene 3,20 dione 21 aminosteroid neuromodulin reduced nicotinamide adenine dinucleotide phosphate dehydrogenase amyotrophic lateral sclerosis animal experiment animal model article controlled study drug effect enzyme inhibition glia cell immunocytochemistry motoneuron mouse nerve fiber regeneration nerve sprouting nonhuman oxidative stress priority journal protein expression spinal cord Amyotrophic Lateral Sclerosis Animals Antioxidants Dihydrolipoamide Dehydrogenase Disease Models, Animal Female GAP-43 Protein Gene Expression Regulation Humans Male Mice Mice, Neurologic Mutants Motor Neurons Pregnatrienes Spinal Cord Animalia The 21-aminosteroid U-74389F attenuates hyperexpression of GAP-43 and NADPH-diaphorase in the spinal cord of wobbler mouse, a model for amyotrophic lateral sclerosis |
| topic_facet |
21-aminosteroids GAP-43 NADPH-diaphorase Neurogeneration Wobbler mouse 21 [4 [2,6 bis(1 pyrrolidinyl) 4 pyrimidinyl] 1 piperazinyl]pregna 1,4,9(11) triene 3,20 dione 21 aminosteroid neuromodulin reduced nicotinamide adenine dinucleotide phosphate dehydrogenase amyotrophic lateral sclerosis animal experiment animal model article controlled study drug effect enzyme inhibition glia cell immunocytochemistry motoneuron mouse nerve fiber regeneration nerve sprouting nonhuman oxidative stress priority journal protein expression spinal cord Amyotrophic Lateral Sclerosis Animals Antioxidants Dihydrolipoamide Dehydrogenase Disease Models, Animal Female GAP-43 Protein Gene Expression Regulation Humans Male Mice Mice, Neurologic Mutants Motor Neurons Pregnatrienes Spinal Cord Animalia |
| description |
The wobbler mouse suffers an autosomal recessive mutation producing severe neurodegeneration and astrogliosis in spinal cord. It has been considered a model for amyotrophic lateral sclerosis. We have studied in these animals the expression of two proteins, the growth-associated protein (GAP-43) and the NADPH-diaphorase, the nitric oxide synthesizing enzyme, employing immunocytochemistry and histochemistry. We found higher expression of GAP-43 immunoreactivity in dorsal horn, Lamina X, corticospinal tract and ventral horn motoneurons in wobbler mice compared to controls. We NADPH- diaphorase activity was present in control motoneurons, in contrast to intense labeling of the wobbler group. No differences in diaphorase activity was measured in the rest of the spinal cord between control and mutant mice. A group of animals received subcutaneously for 4 days a 50 mg pellet of U- 74389F, a glucocorticoid-derived 21-aminosteroid with antioxidant properties but without glucocorticoid activity. U-74389F slightly attenuated GAP-43 immunostaining in dorsal regions of the spinal cord from wobblers but not in controls. However, in motoneurons of wobbler mice number of GAP-43 immunopositive neurons, cell processes and reaction intensity were reduced by U-74389F. The aminosteroid reduced by 50% motoneuron NADPH-diaphorase activity. Hyperexpression of GAP-43 immunoreactivity in wobbler mice may represent an exaggerated neuronal response to advancing degeneration or muscle denervation. It may also be linked to increased nitric oxide levels. U-74389F may stop neurodegeneration and/or increase muscle trophism and stop oxidative stress, consequently GAP-43 hyperexpression was attenuated. Wobbler mice may be important models to evaluate the use of antioxidant steroid therapy with a view to its use in human motoneuron disease. |
| title |
The 21-aminosteroid U-74389F attenuates hyperexpression of GAP-43 and NADPH-diaphorase in the spinal cord of wobbler mouse, a model for amyotrophic lateral sclerosis |
| title_short |
The 21-aminosteroid U-74389F attenuates hyperexpression of GAP-43 and NADPH-diaphorase in the spinal cord of wobbler mouse, a model for amyotrophic lateral sclerosis |
| title_full |
The 21-aminosteroid U-74389F attenuates hyperexpression of GAP-43 and NADPH-diaphorase in the spinal cord of wobbler mouse, a model for amyotrophic lateral sclerosis |
| title_fullStr |
The 21-aminosteroid U-74389F attenuates hyperexpression of GAP-43 and NADPH-diaphorase in the spinal cord of wobbler mouse, a model for amyotrophic lateral sclerosis |
| title_full_unstemmed |
The 21-aminosteroid U-74389F attenuates hyperexpression of GAP-43 and NADPH-diaphorase in the spinal cord of wobbler mouse, a model for amyotrophic lateral sclerosis |
| title_sort |
21-aminosteroid u-74389f attenuates hyperexpression of gap-43 and nadph-diaphorase in the spinal cord of wobbler mouse, a model for amyotrophic lateral sclerosis |
| publishDate |
1999 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v24_n1_p1_GonzalezDeniselle http://hdl.handle.net/20.500.12110/paper_03643190_v24_n1_p1_GonzalezDeniselle |
| _version_ |
1768544642626748416 |