In vitro differences between astrocytes of control and Wobbler mice spinal cord
The Wobbler mouse, a model of amyotrophic lateral sclerosis (ALS), presents motorneuron degeneration and pronounced astrogliosis in the spinal cord. We have studied factors controlling astrocyte proliferation in cultures derived from Wobbler and control mice spinal cord. Basal rate of [3H]thymidine...
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1999
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v24_n12_p1535_GonzalezDeniselle http://hdl.handle.net/20.500.12110/paper_03643190_v24_n12_p1535_GonzalezDeniselle |
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paper:paper_03643190_v24_n12_p1535_GonzalezDeniselle2023-06-08T15:35:34Z In vitro differences between astrocytes of control and Wobbler mice spinal cord Astrocytes Culture Glial fibrillary acidic protein Glucocorticoids Interleukin 1 TGF-β1 Wobbler mouse corticosterone glial fibrillary acidic protein interleukin 1alpha transforming growth factor beta1 amyotrophic lateral sclerosis animal cell article astrocyte astrocytosis cell proliferation controlled study female male motoneuron mouse nerve cell degeneration nonhuman priority journal protein expression spinal cord atrophy Animals Astrocytes Cell Division Cells, Cultured Corticosterone Female Glial Fibrillary Acidic Protein Gliosis Interleukin-1 Kinetics Male Mice Mice, Inbred Strains Mice, Neurologic Mutants Motor Neuron Disease Neuroprotective Agents Pregnatrienes Receptors, Glucocorticoid Reference Values Spinal Cord Thymidine Transforming Growth Factor beta Animalia The Wobbler mouse, a model of amyotrophic lateral sclerosis (ALS), presents motorneuron degeneration and pronounced astrogliosis in the spinal cord. We have studied factors controlling astrocyte proliferation in cultures derived from Wobbler and control mice spinal cord. Basal rate of [3H]thymidine incorporation was 15 times lower in Wobbler astrocytes. While in control cultured cells interleukin-1α (IL-1) and corticosterone (CORT) significantly increased proliferation, both agents were inactive in Wobbler astrocytes. The lack of response to CORT was not due to the absence of glucocorticoid receptors, because similar receptor amounts were found in Wobbler and control astrocytes. In contrast to IL-1 and CORT, transforming growth factor-β1 (TGF-β1) substantially increased proliferation of Wobbler astrocytes but not of control cells. Differences in response to TGF- β1 were also obtained by measuring glial fibrillary acidic protein (GFAP) immunoreaction intensity, which was substantially higher in Wobbler astrocytes. Thus, abnormal responses to different mitogens characterized Wobbler astrocytes in culture. We suggest that TGF-β1 may play a role in the reactive gliosis and GFAP hyperexpression found in the degenerating spinal cord of this model of ALS. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v24_n12_p1535_GonzalezDeniselle http://hdl.handle.net/20.500.12110/paper_03643190_v24_n12_p1535_GonzalezDeniselle |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Astrocytes Culture Glial fibrillary acidic protein Glucocorticoids Interleukin 1 TGF-β1 Wobbler mouse corticosterone glial fibrillary acidic protein interleukin 1alpha transforming growth factor beta1 amyotrophic lateral sclerosis animal cell article astrocyte astrocytosis cell proliferation controlled study female male motoneuron mouse nerve cell degeneration nonhuman priority journal protein expression spinal cord atrophy Animals Astrocytes Cell Division Cells, Cultured Corticosterone Female Glial Fibrillary Acidic Protein Gliosis Interleukin-1 Kinetics Male Mice Mice, Inbred Strains Mice, Neurologic Mutants Motor Neuron Disease Neuroprotective Agents Pregnatrienes Receptors, Glucocorticoid Reference Values Spinal Cord Thymidine Transforming Growth Factor beta Animalia |
| spellingShingle |
Astrocytes Culture Glial fibrillary acidic protein Glucocorticoids Interleukin 1 TGF-β1 Wobbler mouse corticosterone glial fibrillary acidic protein interleukin 1alpha transforming growth factor beta1 amyotrophic lateral sclerosis animal cell article astrocyte astrocytosis cell proliferation controlled study female male motoneuron mouse nerve cell degeneration nonhuman priority journal protein expression spinal cord atrophy Animals Astrocytes Cell Division Cells, Cultured Corticosterone Female Glial Fibrillary Acidic Protein Gliosis Interleukin-1 Kinetics Male Mice Mice, Inbred Strains Mice, Neurologic Mutants Motor Neuron Disease Neuroprotective Agents Pregnatrienes Receptors, Glucocorticoid Reference Values Spinal Cord Thymidine Transforming Growth Factor beta Animalia In vitro differences between astrocytes of control and Wobbler mice spinal cord |
| topic_facet |
Astrocytes Culture Glial fibrillary acidic protein Glucocorticoids Interleukin 1 TGF-β1 Wobbler mouse corticosterone glial fibrillary acidic protein interleukin 1alpha transforming growth factor beta1 amyotrophic lateral sclerosis animal cell article astrocyte astrocytosis cell proliferation controlled study female male motoneuron mouse nerve cell degeneration nonhuman priority journal protein expression spinal cord atrophy Animals Astrocytes Cell Division Cells, Cultured Corticosterone Female Glial Fibrillary Acidic Protein Gliosis Interleukin-1 Kinetics Male Mice Mice, Inbred Strains Mice, Neurologic Mutants Motor Neuron Disease Neuroprotective Agents Pregnatrienes Receptors, Glucocorticoid Reference Values Spinal Cord Thymidine Transforming Growth Factor beta Animalia |
| description |
The Wobbler mouse, a model of amyotrophic lateral sclerosis (ALS), presents motorneuron degeneration and pronounced astrogliosis in the spinal cord. We have studied factors controlling astrocyte proliferation in cultures derived from Wobbler and control mice spinal cord. Basal rate of [3H]thymidine incorporation was 15 times lower in Wobbler astrocytes. While in control cultured cells interleukin-1α (IL-1) and corticosterone (CORT) significantly increased proliferation, both agents were inactive in Wobbler astrocytes. The lack of response to CORT was not due to the absence of glucocorticoid receptors, because similar receptor amounts were found in Wobbler and control astrocytes. In contrast to IL-1 and CORT, transforming growth factor-β1 (TGF-β1) substantially increased proliferation of Wobbler astrocytes but not of control cells. Differences in response to TGF- β1 were also obtained by measuring glial fibrillary acidic protein (GFAP) immunoreaction intensity, which was substantially higher in Wobbler astrocytes. Thus, abnormal responses to different mitogens characterized Wobbler astrocytes in culture. We suggest that TGF-β1 may play a role in the reactive gliosis and GFAP hyperexpression found in the degenerating spinal cord of this model of ALS. |
| title |
In vitro differences between astrocytes of control and Wobbler mice spinal cord |
| title_short |
In vitro differences between astrocytes of control and Wobbler mice spinal cord |
| title_full |
In vitro differences between astrocytes of control and Wobbler mice spinal cord |
| title_fullStr |
In vitro differences between astrocytes of control and Wobbler mice spinal cord |
| title_full_unstemmed |
In vitro differences between astrocytes of control and Wobbler mice spinal cord |
| title_sort |
in vitro differences between astrocytes of control and wobbler mice spinal cord |
| publishDate |
1999 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03643190_v24_n12_p1535_GonzalezDeniselle http://hdl.handle.net/20.500.12110/paper_03643190_v24_n12_p1535_GonzalezDeniselle |
| _version_ |
1768544914106220544 |