Nitric oxide reduces paracellular resistance in rat thick ascending limbs by increasing Na+ and Cl- permeabilities
About 50% of the Na+ reabsorbed in thick ascending limbs traverses the paracellular pathway. Nitric oxide (NO) reduces the permselectivity of this pathway via cGMP, but its effects on absolute Na+ (PNa +) and Cl- (PCl -) permeabilities are unknown. To address this, we measured the effect of L-argini...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03636127_v312_n6_pF1035_Monzon http://hdl.handle.net/20.500.12110/paper_03636127_v312_n6_pF1035_Monzon |
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paper:paper_03636127_v312_n6_pF1035_Monzon2023-06-08T15:35:27Z Nitric oxide reduces paracellular resistance in rat thick ascending limbs by increasing Na+ and Cl- permeabilities Kidney Nitric oxide Paracellular permeability Sodium transport chloride nitric oxide sodium arginine chloride cyclic GMP enzyme inhibitor n(g) nitroarginine methyl ester nitric oxide nitric oxide synthase sodium animal experiment Article cell membrane permeability conductance controlled study electric potential limb male Michaelis Menten kinetics nonhuman osmolality priority journal rat steady state transcytosis transepithelial resistance velocity animal antagonists and inhibitors biological model drug effects Henle loop impedance in vitro study kidney tubule absorption metabolism perfusion permeability Sprague Dawley rat transport at the cellular level Animals Arginine Biological Transport Chlorides Cyclic GMP Electric Impedance Enzyme Inhibitors In Vitro Techniques Loop of Henle Male Models, Biological NG-Nitroarginine Methyl Ester Nitric Oxide Nitric Oxide Synthase Perfusion Permeability Rats, Sprague-Dawley Renal Reabsorption Sodium About 50% of the Na+ reabsorbed in thick ascending limbs traverses the paracellular pathway. Nitric oxide (NO) reduces the permselectivity of this pathway via cGMP, but its effects on absolute Na+ (PNa +) and Cl- (PCl -) permeabilities are unknown. To address this, we measured the effect of L-arginine (0.5 mmol/l; NO synthase substrate) and cGMP (0.5 mmol/l) on PNa + and PCl - calculated from the transepithelial resistance (Rt) and PNa +/PCl - in medullary thick ascending limbs. Rt was 7,722 ± 1,554 ohm·cm in the control period and 6,318 ± 1,757 ohm·cm after L-arginine treatment (P < 0.05). PNa +/PCl - was 2.0 ± 0.2 in the control period and 1.7 ± 0.1 after L-arginine (P < 0.04). Calculated PNa + and PCl - were 3.52 ± 0.2 and 1.81 ± 0.10 × 10-5 cm/s, respectively, in the control period. After L-arginine they were 6.65 ± 0.69 (P < 0.0001 vs. control) and 3.97 ± 0.44 (P < 0.0001) × 10-5 cm/s, respectively. NOS inhibition with Nω-nitro-L-arginine methyl ester (5 mmol/l) prevented L-arginine’s effect on Rt. Next we tested the effect of cGMP. Rt in the control period was 7,592 ± 1,470 and 4,796 ± 847 ohm·cm after dibutyryl-cGMP (0.5 mmol/l; db-cGMP) treatment (P < 0.04). PNa +/PCl - was 1.8 ± 0.1 in the control period and 1.6 ± 0.1 after db-cGMP (P < 0.03). PNa + and PCl - were 4.58 ± 0.80 and 2.66 ± 0.57 × 10-5 cm/s, respectively, for the control period and 9.48 ± 1.63 (P < 0.007) and 6.01 ± 1.05 (P < 0.005) × 10-5 cm/s, respectively, after db-cGMP. We modeled NO’s effect on luminal Na+ concentration along the thick ascending limb. We found that NO’s effect on the paracellular pathway reduces net Na+ reabsorption and that the magnitude of this effect is similar to that due to NO’s inhibition of transcellular transport. © 2017 the American Physiological Society. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03636127_v312_n6_pF1035_Monzon http://hdl.handle.net/20.500.12110/paper_03636127_v312_n6_pF1035_Monzon |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Kidney Nitric oxide Paracellular permeability Sodium transport chloride nitric oxide sodium arginine chloride cyclic GMP enzyme inhibitor n(g) nitroarginine methyl ester nitric oxide nitric oxide synthase sodium animal experiment Article cell membrane permeability conductance controlled study electric potential limb male Michaelis Menten kinetics nonhuman osmolality priority journal rat steady state transcytosis transepithelial resistance velocity animal antagonists and inhibitors biological model drug effects Henle loop impedance in vitro study kidney tubule absorption metabolism perfusion permeability Sprague Dawley rat transport at the cellular level Animals Arginine Biological Transport Chlorides Cyclic GMP Electric Impedance Enzyme Inhibitors In Vitro Techniques Loop of Henle Male Models, Biological NG-Nitroarginine Methyl Ester Nitric Oxide Nitric Oxide Synthase Perfusion Permeability Rats, Sprague-Dawley Renal Reabsorption Sodium |
| spellingShingle |
Kidney Nitric oxide Paracellular permeability Sodium transport chloride nitric oxide sodium arginine chloride cyclic GMP enzyme inhibitor n(g) nitroarginine methyl ester nitric oxide nitric oxide synthase sodium animal experiment Article cell membrane permeability conductance controlled study electric potential limb male Michaelis Menten kinetics nonhuman osmolality priority journal rat steady state transcytosis transepithelial resistance velocity animal antagonists and inhibitors biological model drug effects Henle loop impedance in vitro study kidney tubule absorption metabolism perfusion permeability Sprague Dawley rat transport at the cellular level Animals Arginine Biological Transport Chlorides Cyclic GMP Electric Impedance Enzyme Inhibitors In Vitro Techniques Loop of Henle Male Models, Biological NG-Nitroarginine Methyl Ester Nitric Oxide Nitric Oxide Synthase Perfusion Permeability Rats, Sprague-Dawley Renal Reabsorption Sodium Nitric oxide reduces paracellular resistance in rat thick ascending limbs by increasing Na+ and Cl- permeabilities |
| topic_facet |
Kidney Nitric oxide Paracellular permeability Sodium transport chloride nitric oxide sodium arginine chloride cyclic GMP enzyme inhibitor n(g) nitroarginine methyl ester nitric oxide nitric oxide synthase sodium animal experiment Article cell membrane permeability conductance controlled study electric potential limb male Michaelis Menten kinetics nonhuman osmolality priority journal rat steady state transcytosis transepithelial resistance velocity animal antagonists and inhibitors biological model drug effects Henle loop impedance in vitro study kidney tubule absorption metabolism perfusion permeability Sprague Dawley rat transport at the cellular level Animals Arginine Biological Transport Chlorides Cyclic GMP Electric Impedance Enzyme Inhibitors In Vitro Techniques Loop of Henle Male Models, Biological NG-Nitroarginine Methyl Ester Nitric Oxide Nitric Oxide Synthase Perfusion Permeability Rats, Sprague-Dawley Renal Reabsorption Sodium |
| description |
About 50% of the Na+ reabsorbed in thick ascending limbs traverses the paracellular pathway. Nitric oxide (NO) reduces the permselectivity of this pathway via cGMP, but its effects on absolute Na+ (PNa +) and Cl- (PCl -) permeabilities are unknown. To address this, we measured the effect of L-arginine (0.5 mmol/l; NO synthase substrate) and cGMP (0.5 mmol/l) on PNa + and PCl - calculated from the transepithelial resistance (Rt) and PNa +/PCl - in medullary thick ascending limbs. Rt was 7,722 ± 1,554 ohm·cm in the control period and 6,318 ± 1,757 ohm·cm after L-arginine treatment (P < 0.05). PNa +/PCl - was 2.0 ± 0.2 in the control period and 1.7 ± 0.1 after L-arginine (P < 0.04). Calculated PNa + and PCl - were 3.52 ± 0.2 and 1.81 ± 0.10 × 10-5 cm/s, respectively, in the control period. After L-arginine they were 6.65 ± 0.69 (P < 0.0001 vs. control) and 3.97 ± 0.44 (P < 0.0001) × 10-5 cm/s, respectively. NOS inhibition with Nω-nitro-L-arginine methyl ester (5 mmol/l) prevented L-arginine’s effect on Rt. Next we tested the effect of cGMP. Rt in the control period was 7,592 ± 1,470 and 4,796 ± 847 ohm·cm after dibutyryl-cGMP (0.5 mmol/l; db-cGMP) treatment (P < 0.04). PNa +/PCl - was 1.8 ± 0.1 in the control period and 1.6 ± 0.1 after db-cGMP (P < 0.03). PNa + and PCl - were 4.58 ± 0.80 and 2.66 ± 0.57 × 10-5 cm/s, respectively, for the control period and 9.48 ± 1.63 (P < 0.007) and 6.01 ± 1.05 (P < 0.005) × 10-5 cm/s, respectively, after db-cGMP. We modeled NO’s effect on luminal Na+ concentration along the thick ascending limb. We found that NO’s effect on the paracellular pathway reduces net Na+ reabsorption and that the magnitude of this effect is similar to that due to NO’s inhibition of transcellular transport. © 2017 the American Physiological Society. |
| title |
Nitric oxide reduces paracellular resistance in rat thick ascending limbs by increasing Na+ and Cl- permeabilities |
| title_short |
Nitric oxide reduces paracellular resistance in rat thick ascending limbs by increasing Na+ and Cl- permeabilities |
| title_full |
Nitric oxide reduces paracellular resistance in rat thick ascending limbs by increasing Na+ and Cl- permeabilities |
| title_fullStr |
Nitric oxide reduces paracellular resistance in rat thick ascending limbs by increasing Na+ and Cl- permeabilities |
| title_full_unstemmed |
Nitric oxide reduces paracellular resistance in rat thick ascending limbs by increasing Na+ and Cl- permeabilities |
| title_sort |
nitric oxide reduces paracellular resistance in rat thick ascending limbs by increasing na+ and cl- permeabilities |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03636127_v312_n6_pF1035_Monzon http://hdl.handle.net/20.500.12110/paper_03636127_v312_n6_pF1035_Monzon |
| _version_ |
1768544275318964224 |