Apoptosis, proliferation and presence of estradiol receptors in the testes and Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura)

The dynamic equilibrium between spermatogonial proliferation and testicular apoptosis determines the progression of spermatogenesis in amphibians. Estrogens and their receptors play a central role in regulating spermatogenesis in vertebrates, and in some species of anurans, estradiol (E2) is involve...

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Detalles Bibliográficos
Autores principales: Scaia, María Florencia, Ceballos, Nora Raquel
Publicado: 2016
Materias:
Amphibians
Bufonidae
Estrogens
Reproduction
Spermatogenesis
estradiol receptor
estrogen receptor beta
anatomy and histology
animal
apoptosis
cell proliferation
cytology
male
metabolism
physiology
Sertoli cell
spermatogenesis
testis
Animals
Apoptosis
Cell Proliferation
Estrogen Receptor beta
Male
Receptors, Estradiol
Sertoli Cells
Testis
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03622525_v277_n4_p412_Scaia
http://hdl.handle.net/20.500.12110/paper_03622525_v277_n4_p412_Scaia
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling paper:paper_03622525_v277_n4_p412_Scaia2023-06-08T15:35:04Z Apoptosis, proliferation and presence of estradiol receptors in the testes and Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura) Scaia, María Florencia Ceballos, Nora Raquel Amphibians Bufonidae Estrogens Reproduction Spermatogenesis estradiol receptor estrogen receptor beta anatomy and histology animal apoptosis Bufonidae cell proliferation cytology male metabolism physiology Sertoli cell spermatogenesis testis Animals Apoptosis Bufonidae Cell Proliferation Estrogen Receptor beta Male Receptors, Estradiol Sertoli Cells Spermatogenesis Testis The dynamic equilibrium between spermatogonial proliferation and testicular apoptosis determines the progression of spermatogenesis in amphibians. Estrogens and their receptors play a central role in regulating spermatogenesis in vertebrates, and in some species of anurans, estradiol (E2) is involved in the regulation of spermatogonial proliferation and apoptosis of germ cells. Bidder's organ (BO) is a structure characteristic of Bufonidae that has historically been compared to an undeveloped ovary. In adult Rhinella arenarum males, BO is one of the main sources of plasma E2. The aim of this study was 1) to describe the seasonal variations in testicular apoptosis, spermatogonial proliferation, and cellular proliferation in BO; and 2) to analyze the presence and localization of estrogen receptor β (ERβ) in the testes and BO of R. arenarum. Testicular fragments and BOs from animals collected during the year were labeled with 5-bromo-2′-deoxyuridine (BrdU) and BrdU incorporation was determined using immunohistochemistry. Apoptosis in testicular sections was detected using the TUNEL method, and ERβ localization was assessed using immunohistochemistry in testes and BOs. The results indicate that spermatogonial proliferation is highest during the reproductive season and that cysts of spermatocytes and spermatids undergo apoptosis during the postreproductive season. Furthermore, the proliferation of follicular cells is highest during the reproductive and postreproductive seasons. ERβ was primarily detected by immunolocalization in Sertoli cells, follicular cells, and oocytes. Taken together, these results suggest that cysts that do not form spermatozoa are removed from testes by apoptosis and that estrogens regulate both spermatogenesis and oogenesis in adult males of R. arenarum. © 2016 Wiley Periodicals, Inc. Fil:Scaia, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03622525_v277_n4_p412_Scaia http://hdl.handle.net/20.500.12110/paper_03622525_v277_n4_p412_Scaia
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Amphibians
Bufonidae
Estrogens
Reproduction
Spermatogenesis
estradiol receptor
estrogen receptor beta
anatomy and histology
animal
apoptosis
Bufonidae
cell proliferation
cytology
male
metabolism
physiology
Sertoli cell
spermatogenesis
testis
Animals
Apoptosis
Bufonidae
Cell Proliferation
Estrogen Receptor beta
Male
Receptors, Estradiol
Sertoli Cells
Spermatogenesis
Testis
spellingShingle Amphibians
Bufonidae
Estrogens
Reproduction
Spermatogenesis
estradiol receptor
estrogen receptor beta
anatomy and histology
animal
apoptosis
Bufonidae
cell proliferation
cytology
male
metabolism
physiology
Sertoli cell
spermatogenesis
testis
Animals
Apoptosis
Bufonidae
Cell Proliferation
Estrogen Receptor beta
Male
Receptors, Estradiol
Sertoli Cells
Spermatogenesis
Testis
Scaia, María Florencia
Ceballos, Nora Raquel
Apoptosis, proliferation and presence of estradiol receptors in the testes and Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura)
topic_facet Amphibians
Bufonidae
Estrogens
Reproduction
Spermatogenesis
estradiol receptor
estrogen receptor beta
anatomy and histology
animal
apoptosis
Bufonidae
cell proliferation
cytology
male
metabolism
physiology
Sertoli cell
spermatogenesis
testis
Animals
Apoptosis
Bufonidae
Cell Proliferation
Estrogen Receptor beta
Male
Receptors, Estradiol
Sertoli Cells
Spermatogenesis
Testis
description The dynamic equilibrium between spermatogonial proliferation and testicular apoptosis determines the progression of spermatogenesis in amphibians. Estrogens and their receptors play a central role in regulating spermatogenesis in vertebrates, and in some species of anurans, estradiol (E2) is involved in the regulation of spermatogonial proliferation and apoptosis of germ cells. Bidder's organ (BO) is a structure characteristic of Bufonidae that has historically been compared to an undeveloped ovary. In adult Rhinella arenarum males, BO is one of the main sources of plasma E2. The aim of this study was 1) to describe the seasonal variations in testicular apoptosis, spermatogonial proliferation, and cellular proliferation in BO; and 2) to analyze the presence and localization of estrogen receptor β (ERβ) in the testes and BO of R. arenarum. Testicular fragments and BOs from animals collected during the year were labeled with 5-bromo-2′-deoxyuridine (BrdU) and BrdU incorporation was determined using immunohistochemistry. Apoptosis in testicular sections was detected using the TUNEL method, and ERβ localization was assessed using immunohistochemistry in testes and BOs. The results indicate that spermatogonial proliferation is highest during the reproductive season and that cysts of spermatocytes and spermatids undergo apoptosis during the postreproductive season. Furthermore, the proliferation of follicular cells is highest during the reproductive and postreproductive seasons. ERβ was primarily detected by immunolocalization in Sertoli cells, follicular cells, and oocytes. Taken together, these results suggest that cysts that do not form spermatozoa are removed from testes by apoptosis and that estrogens regulate both spermatogenesis and oogenesis in adult males of R. arenarum. © 2016 Wiley Periodicals, Inc.
author Scaia, María Florencia
Ceballos, Nora Raquel
author_facet Scaia, María Florencia
Ceballos, Nora Raquel
author_sort Scaia, María Florencia
title Apoptosis, proliferation and presence of estradiol receptors in the testes and Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura)
title_short Apoptosis, proliferation and presence of estradiol receptors in the testes and Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura)
title_full Apoptosis, proliferation and presence of estradiol receptors in the testes and Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura)
title_fullStr Apoptosis, proliferation and presence of estradiol receptors in the testes and Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura)
title_full_unstemmed Apoptosis, proliferation and presence of estradiol receptors in the testes and Bidder's organ of the toad Rhinella arenarum (Amphibia, Anura)
title_sort apoptosis, proliferation and presence of estradiol receptors in the testes and bidder's organ of the toad rhinella arenarum (amphibia, anura)
publishDate 2016
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03622525_v277_n4_p412_Scaia
http://hdl.handle.net/20.500.12110/paper_03622525_v277_n4_p412_Scaia
work_keys_str_mv AT scaiamariaflorencia apoptosisproliferationandpresenceofestradiolreceptorsinthetestesandbiddersorganofthetoadrhinellaarenarumamphibiaanura
AT ceballosnoraraquel apoptosisproliferationandpresenceofestradiolreceptorsinthetestesandbiddersorganofthetoadrhinellaarenarumamphibiaanura
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