Anteroposterior variation of the cell types in the interrenal gland of the male toad Rhinella arenarum (Amphibia, Anura)
The interrenal gland of anurans synthesizes the steroids aldosterone and corticosterone, but it is unknown whether these hormones are synthesized by the same cell type. In this work, we aim to elucidate whether there are different steroidogenic cell types and whether they have specific regionalizati...
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2013
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03622525_v274_n3_p331_Regueira http://hdl.handle.net/20.500.12110/paper_03622525_v274_n3_p331_Regueira |
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paper:paper_03622525_v274_n3_p331_Regueira2023-06-08T15:35:03Z Anteroposterior variation of the cell types in the interrenal gland of the male toad Rhinella arenarum (Amphibia, Anura) Regueira, Eleonora Scaia, María Florencia Volonteri, María Clara Ceballos, Nora Raquel Anuran Histology Interrenal gland Radioimmunoassay Steroidogenic cells Transmission electron microscopy aldosterone corticosterone animal Anura article biosynthesis cell nucleus chromaffin cell cytology echography histology immunohistochemistry interrenal gland male metabolism ultrastructure Aldosterone Animals Anura Cell Nucleus Chromaffin Cells Corticosterone Immunohistochemistry Interrenal Gland Male Amphibia Anura The interrenal gland of anurans synthesizes the steroids aldosterone and corticosterone, but it is unknown whether these hormones are synthesized by the same cell type. In this work, we aim to elucidate whether there are different steroidogenic cell types and whether they have specific regionalization in the interrenal gland of the male toad Rhinella arenarum. We characterized all cell types using histological, immuhistochemical, and histochemical methods as well as transmission electron microscopy. Furthermore, we evaluated the organization of the cell types in the gland and anteroposterior variations in the synthesis of the steroids. We found evidence of five cell types: two morphologically different steroidogenic cells, type 1: polyhedral cells tightly attached to each other that have spherical euchromatic nuclei and type 2: retracted cells loosely attached to each other that have oval heterochromatic nuclei. Cell type 2 is mainly observed in the inner zone of the gland. In addition, we observed two types of chromaffin cells, called type 3 and 4 cells, randomly distributed throughout the interrenal gland, as well as type 5 cells, recognized as summer cells. Morphometric analyses of the cell types in the anterior and posterior zones of the interrenal showed that the ratio "area of type 2 cells/total interrenal area" is significantly lower in the posterior zone. In vitro incubations showed that the posterior portion of the gland produces significantly higher amounts of both corticosterone and aldosterone. Overall, our results suggest that the type 2 cells are less active to synthesize both aldosterone and corticosterone, compared to type 1 cells. Unlike most previous reports on the interrenal gland of anurans, in R. arenarum there is a zonation of the steroidogenic cell types, which implies that the organ is not anteroposterior or dorsoventrally homogeneous. © 2012 Wiley Periodicals, Inc. Fil:Regueira, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scaia, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Volonteri, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03622525_v274_n3_p331_Regueira http://hdl.handle.net/20.500.12110/paper_03622525_v274_n3_p331_Regueira |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Anuran Histology Interrenal gland Radioimmunoassay Steroidogenic cells Transmission electron microscopy aldosterone corticosterone animal Anura article biosynthesis cell nucleus chromaffin cell cytology echography histology immunohistochemistry interrenal gland male metabolism ultrastructure Aldosterone Animals Anura Cell Nucleus Chromaffin Cells Corticosterone Immunohistochemistry Interrenal Gland Male Amphibia Anura |
| spellingShingle |
Anuran Histology Interrenal gland Radioimmunoassay Steroidogenic cells Transmission electron microscopy aldosterone corticosterone animal Anura article biosynthesis cell nucleus chromaffin cell cytology echography histology immunohistochemistry interrenal gland male metabolism ultrastructure Aldosterone Animals Anura Cell Nucleus Chromaffin Cells Corticosterone Immunohistochemistry Interrenal Gland Male Amphibia Anura Regueira, Eleonora Scaia, María Florencia Volonteri, María Clara Ceballos, Nora Raquel Anteroposterior variation of the cell types in the interrenal gland of the male toad Rhinella arenarum (Amphibia, Anura) |
| topic_facet |
Anuran Histology Interrenal gland Radioimmunoassay Steroidogenic cells Transmission electron microscopy aldosterone corticosterone animal Anura article biosynthesis cell nucleus chromaffin cell cytology echography histology immunohistochemistry interrenal gland male metabolism ultrastructure Aldosterone Animals Anura Cell Nucleus Chromaffin Cells Corticosterone Immunohistochemistry Interrenal Gland Male Amphibia Anura |
| description |
The interrenal gland of anurans synthesizes the steroids aldosterone and corticosterone, but it is unknown whether these hormones are synthesized by the same cell type. In this work, we aim to elucidate whether there are different steroidogenic cell types and whether they have specific regionalization in the interrenal gland of the male toad Rhinella arenarum. We characterized all cell types using histological, immuhistochemical, and histochemical methods as well as transmission electron microscopy. Furthermore, we evaluated the organization of the cell types in the gland and anteroposterior variations in the synthesis of the steroids. We found evidence of five cell types: two morphologically different steroidogenic cells, type 1: polyhedral cells tightly attached to each other that have spherical euchromatic nuclei and type 2: retracted cells loosely attached to each other that have oval heterochromatic nuclei. Cell type 2 is mainly observed in the inner zone of the gland. In addition, we observed two types of chromaffin cells, called type 3 and 4 cells, randomly distributed throughout the interrenal gland, as well as type 5 cells, recognized as summer cells. Morphometric analyses of the cell types in the anterior and posterior zones of the interrenal showed that the ratio "area of type 2 cells/total interrenal area" is significantly lower in the posterior zone. In vitro incubations showed that the posterior portion of the gland produces significantly higher amounts of both corticosterone and aldosterone. Overall, our results suggest that the type 2 cells are less active to synthesize both aldosterone and corticosterone, compared to type 1 cells. Unlike most previous reports on the interrenal gland of anurans, in R. arenarum there is a zonation of the steroidogenic cell types, which implies that the organ is not anteroposterior or dorsoventrally homogeneous. © 2012 Wiley Periodicals, Inc. |
| author |
Regueira, Eleonora Scaia, María Florencia Volonteri, María Clara Ceballos, Nora Raquel |
| author_facet |
Regueira, Eleonora Scaia, María Florencia Volonteri, María Clara Ceballos, Nora Raquel |
| author_sort |
Regueira, Eleonora |
| title |
Anteroposterior variation of the cell types in the interrenal gland of the male toad Rhinella arenarum (Amphibia, Anura) |
| title_short |
Anteroposterior variation of the cell types in the interrenal gland of the male toad Rhinella arenarum (Amphibia, Anura) |
| title_full |
Anteroposterior variation of the cell types in the interrenal gland of the male toad Rhinella arenarum (Amphibia, Anura) |
| title_fullStr |
Anteroposterior variation of the cell types in the interrenal gland of the male toad Rhinella arenarum (Amphibia, Anura) |
| title_full_unstemmed |
Anteroposterior variation of the cell types in the interrenal gland of the male toad Rhinella arenarum (Amphibia, Anura) |
| title_sort |
anteroposterior variation of the cell types in the interrenal gland of the male toad rhinella arenarum (amphibia, anura) |
| publishDate |
2013 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03622525_v274_n3_p331_Regueira http://hdl.handle.net/20.500.12110/paper_03622525_v274_n3_p331_Regueira |
| work_keys_str_mv |
AT regueiraeleonora anteroposteriorvariationofthecelltypesintheinterrenalglandofthemaletoadrhinellaarenarumamphibiaanura AT scaiamariaflorencia anteroposteriorvariationofthecelltypesintheinterrenalglandofthemaletoadrhinellaarenarumamphibiaanura AT volonterimariaclara anteroposteriorvariationofthecelltypesintheinterrenalglandofthemaletoadrhinellaarenarumamphibiaanura AT ceballosnoraraquel anteroposteriorvariationofthecelltypesintheinterrenalglandofthemaletoadrhinellaarenarumamphibiaanura |
| _version_ |
1768542693814697984 |