Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams
Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) ir...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03603016_v74_n4_p1226_Ibanez http://hdl.handle.net/20.500.12110/paper_03603016_v74_n4_p1226_Ibanez |
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paper:paper_03603016_v74_n4_p1226_Ibanez2023-06-08T15:34:36Z Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams Molinari de Rey, Beatriz Palmieri, Mónica Alejandra Policastro, Lucía Laura Kreiner, Andrés Juan Burlon, Alejandro Davidson, Jorge Davidson, Miguel Durán, Hebe Alicia γH2AX DNA DSB Ionizing radiation LET Melanoma cells Clonogenic assays DNA double strand breaks DNA DSB Gamma source High linear energy transfers In-cell LET LET radiation Linear-quadratic models Low-LET radiation Melanoma cells Monoenergetic protons Phosphorylated histone Postirradiation Survival curves Surviving fractions Cell membranes DNA Energy transfer Genes Ionizing radiation Lithium Nucleic acids Phosphorylation Proton irradiation Protons Radiation shielding Radioactivity Lighting fixtures double stranded DNA histone H2AX animal cell article cancer cell culture cancer radiotherapy cancer survival clonogenic assay DNA determination DNA strand breakage linear energy transfer low energy radiation melanoma melanoma cell mouse nonhuman priority journal protein determination protein phosphorylation proton radiation radiation dose Biological Markers Cell Survival DNA Breaks, Double-Stranded DNA Repair Dose-Response Relationship, Radiation Histones Humans Linear Energy Transfer Lithium Melanoma, Experimental Phosphorylation Protons Radiation Tolerance Radioisotopes Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irradiated with monoenergetic proton and lithium beams and with a gamma source. Survival curves were obtained by clonogenic assay and fitted to the linear-quadratic model. DSBs were evaluated by the detection of phosphorylated histone H2AX (γH2AX) foci at 30 min and 6 h post-irradiation. Results: Survival curves showed the increasing effectiveness of radiation as a function of LET. γH2AX labeling showed an increase in the number of foci vs. dose for all the radiations evaluated. A decrease in the number of foci was found at 6 h post-irradiation for low LET radiation, revealing the repair capacity of DSBs. An increase in the size of γH2AX foci in cells irradiated with lithium beams was found, as compared with gamma and proton irradiations, which could be attributed to the clusters of DSBs induced by high LET radiation. Foci size increased at 6 h post-irradiation for lithium and proton irradiations in relation with persistent DSBs, showing a correlation with surviving fraction. Conclusions: Our results showed the response of B16-F0 cells to charged particle beams evaluated by the detection of γH2AX foci. We conclude that γH2AX foci size is an accurate parameter to correlate the rejoining of DSBs induced by different LET radiations and radiosensitivity. © 2009 Elsevier Inc. All rights reserved. Fil:Molinari, B.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Palmieri, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Policastro, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kreiner, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Burlón, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Davidson, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Davidson, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Durán, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03603016_v74_n4_p1226_Ibanez http://hdl.handle.net/20.500.12110/paper_03603016_v74_n4_p1226_Ibanez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
γH2AX DNA DSB Ionizing radiation LET Melanoma cells Clonogenic assays DNA double strand breaks DNA DSB Gamma source High linear energy transfers In-cell LET LET radiation Linear-quadratic models Low-LET radiation Melanoma cells Monoenergetic protons Phosphorylated histone Postirradiation Survival curves Surviving fractions Cell membranes DNA Energy transfer Genes Ionizing radiation Lithium Nucleic acids Phosphorylation Proton irradiation Protons Radiation shielding Radioactivity Lighting fixtures double stranded DNA histone H2AX animal cell article cancer cell culture cancer radiotherapy cancer survival clonogenic assay DNA determination DNA strand breakage linear energy transfer low energy radiation melanoma melanoma cell mouse nonhuman priority journal protein determination protein phosphorylation proton radiation radiation dose Biological Markers Cell Survival DNA Breaks, Double-Stranded DNA Repair Dose-Response Relationship, Radiation Histones Humans Linear Energy Transfer Lithium Melanoma, Experimental Phosphorylation Protons Radiation Tolerance Radioisotopes |
| spellingShingle |
γH2AX DNA DSB Ionizing radiation LET Melanoma cells Clonogenic assays DNA double strand breaks DNA DSB Gamma source High linear energy transfers In-cell LET LET radiation Linear-quadratic models Low-LET radiation Melanoma cells Monoenergetic protons Phosphorylated histone Postirradiation Survival curves Surviving fractions Cell membranes DNA Energy transfer Genes Ionizing radiation Lithium Nucleic acids Phosphorylation Proton irradiation Protons Radiation shielding Radioactivity Lighting fixtures double stranded DNA histone H2AX animal cell article cancer cell culture cancer radiotherapy cancer survival clonogenic assay DNA determination DNA strand breakage linear energy transfer low energy radiation melanoma melanoma cell mouse nonhuman priority journal protein determination protein phosphorylation proton radiation radiation dose Biological Markers Cell Survival DNA Breaks, Double-Stranded DNA Repair Dose-Response Relationship, Radiation Histones Humans Linear Energy Transfer Lithium Melanoma, Experimental Phosphorylation Protons Radiation Tolerance Radioisotopes Molinari de Rey, Beatriz Palmieri, Mónica Alejandra Policastro, Lucía Laura Kreiner, Andrés Juan Burlon, Alejandro Davidson, Jorge Davidson, Miguel Durán, Hebe Alicia Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams |
| topic_facet |
γH2AX DNA DSB Ionizing radiation LET Melanoma cells Clonogenic assays DNA double strand breaks DNA DSB Gamma source High linear energy transfers In-cell LET LET radiation Linear-quadratic models Low-LET radiation Melanoma cells Monoenergetic protons Phosphorylated histone Postirradiation Survival curves Surviving fractions Cell membranes DNA Energy transfer Genes Ionizing radiation Lithium Nucleic acids Phosphorylation Proton irradiation Protons Radiation shielding Radioactivity Lighting fixtures double stranded DNA histone H2AX animal cell article cancer cell culture cancer radiotherapy cancer survival clonogenic assay DNA determination DNA strand breakage linear energy transfer low energy radiation melanoma melanoma cell mouse nonhuman priority journal protein determination protein phosphorylation proton radiation radiation dose Biological Markers Cell Survival DNA Breaks, Double-Stranded DNA Repair Dose-Response Relationship, Radiation Histones Humans Linear Energy Transfer Lithium Melanoma, Experimental Phosphorylation Protons Radiation Tolerance Radioisotopes |
| description |
Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irradiated with monoenergetic proton and lithium beams and with a gamma source. Survival curves were obtained by clonogenic assay and fitted to the linear-quadratic model. DSBs were evaluated by the detection of phosphorylated histone H2AX (γH2AX) foci at 30 min and 6 h post-irradiation. Results: Survival curves showed the increasing effectiveness of radiation as a function of LET. γH2AX labeling showed an increase in the number of foci vs. dose for all the radiations evaluated. A decrease in the number of foci was found at 6 h post-irradiation for low LET radiation, revealing the repair capacity of DSBs. An increase in the size of γH2AX foci in cells irradiated with lithium beams was found, as compared with gamma and proton irradiations, which could be attributed to the clusters of DSBs induced by high LET radiation. Foci size increased at 6 h post-irradiation for lithium and proton irradiations in relation with persistent DSBs, showing a correlation with surviving fraction. Conclusions: Our results showed the response of B16-F0 cells to charged particle beams evaluated by the detection of γH2AX foci. We conclude that γH2AX foci size is an accurate parameter to correlate the rejoining of DSBs induced by different LET radiations and radiosensitivity. © 2009 Elsevier Inc. All rights reserved. |
| author |
Molinari de Rey, Beatriz Palmieri, Mónica Alejandra Policastro, Lucía Laura Kreiner, Andrés Juan Burlon, Alejandro Davidson, Jorge Davidson, Miguel Durán, Hebe Alicia |
| author_facet |
Molinari de Rey, Beatriz Palmieri, Mónica Alejandra Policastro, Lucía Laura Kreiner, Andrés Juan Burlon, Alejandro Davidson, Jorge Davidson, Miguel Durán, Hebe Alicia |
| author_sort |
Molinari de Rey, Beatriz |
| title |
Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams |
| title_short |
Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams |
| title_full |
Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams |
| title_fullStr |
Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams |
| title_full_unstemmed |
Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams |
| title_sort |
induction and rejoining of dna double strand breaks assessed by h2ax phosphorylation in melanoma cells irradiated with proton and lithium beams |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03603016_v74_n4_p1226_Ibanez http://hdl.handle.net/20.500.12110/paper_03603016_v74_n4_p1226_Ibanez |
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