Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis

Type IV pili (Tfp) are widely distributed adhesins of bacterial surfaces. In plant pathogenic bacteria, Tfp are involved in host colonization and pathogenesis. Xanthomonas citri subsp. citri (Xcc) is the phytopathogen responsible for citrus canker disease. In this work, three Tfp structural genes, f...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Casabuono, Adriana Cristina, Couto, Alicia Susana
Publicado:	2016
Materias:	bacterial protein pilin protein Tfp unclassified drug fimbria protein Article bacterial colonization bacterial gene bacterial growth bacterial plant disease bacterial strain bacterial virulence bacterium adherence biofilm controlled study fimA gene fimA1 gene gene deletion gene identification gene mutation nonhuman pilA gene priority journal protein glycosylation protein purification structure analysis sweet orange Xanthomonas citri Xanthomonas citri subsp. citri Citrus genetics metabolism microbiology pathogenicity plant disease virulence Xanthomonas Bacterial Proteins Fimbriae Proteins Gene Deletion Plant Diseases Virulence
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v73_n6_p904_Petrocelli http://hdl.handle.net/20.500.12110/paper_03438651_v73_n6_p904_Petrocelli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03438651_v73_n6_p904_Petrocelli
record_format	dspace
spelling	paper:paper_03438651_v73_n6_p904_Petrocelli2023-06-08T15:34:34Z Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis Casabuono, Adriana Cristina Couto, Alicia Susana bacterial protein pilin protein Tfp unclassified drug fimbria protein Article bacterial colonization bacterial gene bacterial growth bacterial plant disease bacterial strain bacterial virulence bacterium adherence biofilm controlled study fimA gene fimA1 gene gene deletion gene identification gene mutation nonhuman pilA gene priority journal protein glycosylation protein purification structure analysis sweet orange Xanthomonas citri Xanthomonas citri subsp. citri Citrus gene deletion genetics metabolism microbiology pathogenicity plant disease virulence Xanthomonas Bacterial Proteins Citrus Fimbriae Proteins Gene Deletion Plant Diseases Virulence Xanthomonas Type IV pili (Tfp) are widely distributed adhesins of bacterial surfaces. In plant pathogenic bacteria, Tfp are involved in host colonization and pathogenesis. Xanthomonas citri subsp. citri (Xcc) is the phytopathogen responsible for citrus canker disease. In this work, three Tfp structural genes, fimA, fimA1, and pilA from Xcc were studied. A pilA mutant strain from Xcc (XccΔpilA) was constructed and differences in physiological features, such as motilities, adhesion, and biofilm formation, were observed. A structural study of the purified Tfp fractions from Xcc wild-type and Xcc∆pilA showed that pilins are glycosylated in both strains and that FimA and FimA1 are the main structural components of the pili. Furthermore, smaller lesion symptoms and reduced bacterial growth were produced by Xcc∆pilA in orange plants compared to the wild-type strain. These results indicate that the minor pilin-like gene, pilA, is involved in Tfp performance during the infection process. © 2016, Springer Science+Business Media New York. Fil:Casabuono, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v73_n6_p904_Petrocelli http://hdl.handle.net/20.500.12110/paper_03438651_v73_n6_p904_Petrocelli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bacterial protein pilin protein Tfp unclassified drug fimbria protein Article bacterial colonization bacterial gene bacterial growth bacterial plant disease bacterial strain bacterial virulence bacterium adherence biofilm controlled study fimA gene fimA1 gene gene deletion gene identification gene mutation nonhuman pilA gene priority journal protein glycosylation protein purification structure analysis sweet orange Xanthomonas citri Xanthomonas citri subsp. citri Citrus gene deletion genetics metabolism microbiology pathogenicity plant disease virulence Xanthomonas Bacterial Proteins Citrus Fimbriae Proteins Gene Deletion Plant Diseases Virulence Xanthomonas
spellingShingle	bacterial protein pilin protein Tfp unclassified drug fimbria protein Article bacterial colonization bacterial gene bacterial growth bacterial plant disease bacterial strain bacterial virulence bacterium adherence biofilm controlled study fimA gene fimA1 gene gene deletion gene identification gene mutation nonhuman pilA gene priority journal protein glycosylation protein purification structure analysis sweet orange Xanthomonas citri Xanthomonas citri subsp. citri Citrus gene deletion genetics metabolism microbiology pathogenicity plant disease virulence Xanthomonas Bacterial Proteins Citrus Fimbriae Proteins Gene Deletion Plant Diseases Virulence Xanthomonas Casabuono, Adriana Cristina Couto, Alicia Susana Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
topic_facet	bacterial protein pilin protein Tfp unclassified drug fimbria protein Article bacterial colonization bacterial gene bacterial growth bacterial plant disease bacterial strain bacterial virulence bacterium adherence biofilm controlled study fimA gene fimA1 gene gene deletion gene identification gene mutation nonhuman pilA gene priority journal protein glycosylation protein purification structure analysis sweet orange Xanthomonas citri Xanthomonas citri subsp. citri Citrus gene deletion genetics metabolism microbiology pathogenicity plant disease virulence Xanthomonas Bacterial Proteins Citrus Fimbriae Proteins Gene Deletion Plant Diseases Virulence Xanthomonas
description	Type IV pili (Tfp) are widely distributed adhesins of bacterial surfaces. In plant pathogenic bacteria, Tfp are involved in host colonization and pathogenesis. Xanthomonas citri subsp. citri (Xcc) is the phytopathogen responsible for citrus canker disease. In this work, three Tfp structural genes, fimA, fimA1, and pilA from Xcc were studied. A pilA mutant strain from Xcc (XccΔpilA) was constructed and differences in physiological features, such as motilities, adhesion, and biofilm formation, were observed. A structural study of the purified Tfp fractions from Xcc wild-type and Xcc∆pilA showed that pilins are glycosylated in both strains and that FimA and FimA1 are the main structural components of the pili. Furthermore, smaller lesion symptoms and reduced bacterial growth were produced by Xcc∆pilA in orange plants compared to the wild-type strain. These results indicate that the minor pilin-like gene, pilA, is involved in Tfp performance during the infection process. © 2016, Springer Science+Business Media New York.
author	Casabuono, Adriana Cristina Couto, Alicia Susana
author_facet	Casabuono, Adriana Cristina Couto, Alicia Susana
author_sort	Casabuono, Adriana Cristina
title	Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
title_short	Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
title_full	Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
title_fullStr	Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
title_full_unstemmed	Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
title_sort	deletion of pila, a minor pilin-like gene, from xanthomonas citri subsp. citri influences bacterial physiology and pathogenesis
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v73_n6_p904_Petrocelli http://hdl.handle.net/20.500.12110/paper_03438651_v73_n6_p904_Petrocelli
work_keys_str_mv	AT casabuonoadrianacristina deletionofpilaaminorpilinlikegenefromxanthomonascitrisubspcitriinfluencesbacterialphysiologyandpathogenesis AT coutoaliciasusana deletionofpilaaminorpilinlikegenefromxanthomonascitrisubspcitriinfluencesbacterialphysiologyandpathogenesis
_version_	1768543802233978880

Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis

Ejemplares similares