rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species Pseudomonas oleovorans
The expression of the rpoS gene during PHA depolymerization was monitored in Pseudomonas oleovorans GPol and its mutant defective in PHA degradation by analyzing the tolerance to oxidative and thermal stresses and the RpoS intracellular content. An increase in the tolerance to H2O 2 and heat shock w...
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v48_n6_p396_Ruiz http://hdl.handle.net/20.500.12110/paper_03438651_v48_n6_p396_Ruiz |
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paper:paper_03438651_v48_n6_p396_Ruiz2023-06-08T15:34:32Z rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species Pseudomonas oleovorans Ruiz, Jimena Alicia Méndez, Beatriz Silvia heat shock protein hydrogen peroxide polyhydroxyalkanoic acid article bacterial gene bacterium culture bacterium mutant degradation depolymerization gene expression gene mutation heat shock response nonhuman oxidative stress phaZ gene phenotype priority journal Pseudomonas oleovorans RpoS gene stress thermal stimulation Western blotting Bacteria (microorganisms) Negibacteria Pseudomonas Pseudomonas oleovorans Pseudomonas oleovorans The expression of the rpoS gene during PHA depolymerization was monitored in Pseudomonas oleovorans GPol and its mutant defective in PHA degradation by analyzing the tolerance to oxidative and thermal stresses and the RpoS intracellular content. An increase in the tolerance to H2O 2 and heat shock was observed coincidentally with PHA degradation. Western blotting experiments performed in carbon-starved cultures showed that the RpoS levels were higher in the wild type than in the mutant strain. Complementation of the phaZ mutation restores the wild-type RpoS levels. These results suggest a probable association between PHA depolymerization and the stress tolerance phenotype controlled by RpoS. Fil:Ruiz, J.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v48_n6_p396_Ruiz http://hdl.handle.net/20.500.12110/paper_03438651_v48_n6_p396_Ruiz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
heat shock protein hydrogen peroxide polyhydroxyalkanoic acid article bacterial gene bacterium culture bacterium mutant degradation depolymerization gene expression gene mutation heat shock response nonhuman oxidative stress phaZ gene phenotype priority journal Pseudomonas oleovorans RpoS gene stress thermal stimulation Western blotting Bacteria (microorganisms) Negibacteria Pseudomonas Pseudomonas oleovorans Pseudomonas oleovorans |
| spellingShingle |
heat shock protein hydrogen peroxide polyhydroxyalkanoic acid article bacterial gene bacterium culture bacterium mutant degradation depolymerization gene expression gene mutation heat shock response nonhuman oxidative stress phaZ gene phenotype priority journal Pseudomonas oleovorans RpoS gene stress thermal stimulation Western blotting Bacteria (microorganisms) Negibacteria Pseudomonas Pseudomonas oleovorans Pseudomonas oleovorans Ruiz, Jimena Alicia Méndez, Beatriz Silvia rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species Pseudomonas oleovorans |
| topic_facet |
heat shock protein hydrogen peroxide polyhydroxyalkanoic acid article bacterial gene bacterium culture bacterium mutant degradation depolymerization gene expression gene mutation heat shock response nonhuman oxidative stress phaZ gene phenotype priority journal Pseudomonas oleovorans RpoS gene stress thermal stimulation Western blotting Bacteria (microorganisms) Negibacteria Pseudomonas Pseudomonas oleovorans Pseudomonas oleovorans |
| description |
The expression of the rpoS gene during PHA depolymerization was monitored in Pseudomonas oleovorans GPol and its mutant defective in PHA degradation by analyzing the tolerance to oxidative and thermal stresses and the RpoS intracellular content. An increase in the tolerance to H2O 2 and heat shock was observed coincidentally with PHA degradation. Western blotting experiments performed in carbon-starved cultures showed that the RpoS levels were higher in the wild type than in the mutant strain. Complementation of the phaZ mutation restores the wild-type RpoS levels. These results suggest a probable association between PHA depolymerization and the stress tolerance phenotype controlled by RpoS. |
| author |
Ruiz, Jimena Alicia Méndez, Beatriz Silvia |
| author_facet |
Ruiz, Jimena Alicia Méndez, Beatriz Silvia |
| author_sort |
Ruiz, Jimena Alicia |
| title |
rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species Pseudomonas oleovorans |
| title_short |
rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species Pseudomonas oleovorans |
| title_full |
rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species Pseudomonas oleovorans |
| title_fullStr |
rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species Pseudomonas oleovorans |
| title_full_unstemmed |
rpoS gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species Pseudomonas oleovorans |
| title_sort |
rpos gene expression in carbon-starved cultures of the polyhydroxyalkanoate-accumulating species pseudomonas oleovorans |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v48_n6_p396_Ruiz http://hdl.handle.net/20.500.12110/paper_03438651_v48_n6_p396_Ruiz |
| work_keys_str_mv |
AT ruizjimenaalicia rposgeneexpressionincarbonstarvedculturesofthepolyhydroxyalkanoateaccumulatingspeciespseudomonasoleovorans AT mendezbeatrizsilvia rposgeneexpressionincarbonstarvedculturesofthepolyhydroxyalkanoateaccumulatingspeciespseudomonasoleovorans |
| _version_ |
1768546161519493120 |