Variations of the envelope composition of Bacillus subtilis during growth in hyperosmotic medium

The envelope properties of B. subtilis cultures grown in LB and LBN hyperosmotic media (LB + 1.5 M NaCl) were compared. Since hypertonic cultures showed a Spo-phenotype, a Spo-mutant grown in LB was also analyzed. LBN cultures showed extensive filamentation and presented different sensitivities towa...

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Autores principales: López, Claudia Susana, Ruzal, Sandra Mónica
Publicado: 1998
Materias:
cardiolipin
fosfomycin
glycolipid
lysozyme
membrane lipid
monounsaturated fatty acid
penicillin g
polymyxin b
article
bacillus subtilis
bacterial membrane
fatty acid synthesis
hyperosmolality
membrane binding
membrane structure
nonhuman
phenotype
priority journal
sporogenesis
structure analysis
Bacillus subtilis
Cell Membrane
Culture Media
Fatty Acids
Glycolipids
Hypertonic Solutions
Phospholipids
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v36_n1_p55_Lopez
http://hdl.handle.net/20.500.12110/paper_03438651_v36_n1_p55_Lopez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03438651_v36_n1_p55_Lopez
record_format dspace
spelling paper:paper_03438651_v36_n1_p55_Lopez2023-06-08T15:34:31Z Variations of the envelope composition of Bacillus subtilis during growth in hyperosmotic medium López, Claudia Susana Ruzal, Sandra Mónica cardiolipin fosfomycin glycolipid lysozyme membrane lipid monounsaturated fatty acid penicillin g polymyxin b article bacillus subtilis bacterial membrane fatty acid synthesis hyperosmolality membrane binding membrane structure nonhuman phenotype priority journal sporogenesis structure analysis Bacillus subtilis Cell Membrane Culture Media Fatty Acids Glycolipids Hypertonic Solutions Phospholipids The envelope properties of B. subtilis cultures grown in LB and LBN hyperosmotic media (LB + 1.5 M NaCl) were compared. Since hypertonic cultures showed a Spo-phenotype, a Spo-mutant grown in LB was also analyzed. LBN cultures showed extensive filamentation and presented different sensitivities toward phage infection (φ29 and φ105), or antibiotics whose targets are at wall (lysozyme, penicillin G) or membrane level (polymyxin B, phosphonomycin). Results of the biochemical composition revealed that during hyperosmotic growth, the cell wall increased in thickness, and among the membrane lipids, glycolipid and cardiolipin increased in parallel with a decrease in phosphatidylglycerol. The fatty acid composition was also modified, and an increase in saturated straight chain with a decrease of saturated iso-branched fatty acids was observed. The increase of monounsaturated 18-1 (ω-9) fatty acid was probably related to the absence of sporulation observed in hypertonic media, since its increase has been shown to inhibit the KinA sensor of sporulation. The significance of the other wall and membrane composition variations (and hydrophobic surface properties) in relation to the osmotic adaptation are discussed. Fil:López, C.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v36_n1_p55_Lopez http://hdl.handle.net/20.500.12110/paper_03438651_v36_n1_p55_Lopez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cardiolipin
fosfomycin
glycolipid
lysozyme
membrane lipid
monounsaturated fatty acid
penicillin g
polymyxin b
article
bacillus subtilis
bacterial membrane
fatty acid synthesis
hyperosmolality
membrane binding
membrane structure
nonhuman
phenotype
priority journal
sporogenesis
structure analysis
Bacillus subtilis
Cell Membrane
Culture Media
Fatty Acids
Glycolipids
Hypertonic Solutions
Phospholipids
spellingShingle cardiolipin
fosfomycin
glycolipid
lysozyme
membrane lipid
monounsaturated fatty acid
penicillin g
polymyxin b
article
bacillus subtilis
bacterial membrane
fatty acid synthesis
hyperosmolality
membrane binding
membrane structure
nonhuman
phenotype
priority journal
sporogenesis
structure analysis
Bacillus subtilis
Cell Membrane
Culture Media
Fatty Acids
Glycolipids
Hypertonic Solutions
Phospholipids
López, Claudia Susana
Ruzal, Sandra Mónica
Variations of the envelope composition of Bacillus subtilis during growth in hyperosmotic medium
topic_facet cardiolipin
fosfomycin
glycolipid
lysozyme
membrane lipid
monounsaturated fatty acid
penicillin g
polymyxin b
article
bacillus subtilis
bacterial membrane
fatty acid synthesis
hyperosmolality
membrane binding
membrane structure
nonhuman
phenotype
priority journal
sporogenesis
structure analysis
Bacillus subtilis
Cell Membrane
Culture Media
Fatty Acids
Glycolipids
Hypertonic Solutions
Phospholipids
description The envelope properties of B. subtilis cultures grown in LB and LBN hyperosmotic media (LB + 1.5 M NaCl) were compared. Since hypertonic cultures showed a Spo-phenotype, a Spo-mutant grown in LB was also analyzed. LBN cultures showed extensive filamentation and presented different sensitivities toward phage infection (φ29 and φ105), or antibiotics whose targets are at wall (lysozyme, penicillin G) or membrane level (polymyxin B, phosphonomycin). Results of the biochemical composition revealed that during hyperosmotic growth, the cell wall increased in thickness, and among the membrane lipids, glycolipid and cardiolipin increased in parallel with a decrease in phosphatidylglycerol. The fatty acid composition was also modified, and an increase in saturated straight chain with a decrease of saturated iso-branched fatty acids was observed. The increase of monounsaturated 18-1 (ω-9) fatty acid was probably related to the absence of sporulation observed in hypertonic media, since its increase has been shown to inhibit the KinA sensor of sporulation. The significance of the other wall and membrane composition variations (and hydrophobic surface properties) in relation to the osmotic adaptation are discussed.
author López, Claudia Susana
Ruzal, Sandra Mónica
author_facet López, Claudia Susana
Ruzal, Sandra Mónica
author_sort López, Claudia Susana
title Variations of the envelope composition of Bacillus subtilis during growth in hyperosmotic medium
title_short Variations of the envelope composition of Bacillus subtilis during growth in hyperosmotic medium
title_full Variations of the envelope composition of Bacillus subtilis during growth in hyperosmotic medium
title_fullStr Variations of the envelope composition of Bacillus subtilis during growth in hyperosmotic medium
title_full_unstemmed Variations of the envelope composition of Bacillus subtilis during growth in hyperosmotic medium
title_sort variations of the envelope composition of bacillus subtilis during growth in hyperosmotic medium
publishDate 1998
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v36_n1_p55_Lopez
http://hdl.handle.net/20.500.12110/paper_03438651_v36_n1_p55_Lopez
work_keys_str_mv AT lopezclaudiasusana variationsoftheenvelopecompositionofbacillussubtilisduringgrowthinhyperosmoticmedium
AT ruzalsandramonica variationsoftheenvelopecompositionofbacillussubtilisduringgrowthinhyperosmoticmedium
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