DNA supercoiling and osmoresistance in Bacillus subtilis 168

The importance of the DNA structure for the expression of the osmotic response (osmotolerance) was investigated in Bacillus subtilis 168. Plasmid pUB110 DNA was used as a reporter of the chromosomal DNA topology, and analyses were performed in chloroquine agarose gels. Plasmidic DNA obtained from cu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Alice, Alejandro Fabián
Publicado:	1997
Materias:	dna topoisomerase (atp hydrolysing) novobiocin plasmid dna article bacillus subtilis bacterial growth bacterium mutant dna supercoiling enzyme activity nonhuman osmoregulation priority journal Anti-Bacterial Agents Bacillus subtilis Chloroquine Culture Media DNA Gyrase DNA Topoisomerases, Type II DNA, Bacterial DNA, Superhelical Genes, Reporter Novobiocin Osmotic Pressure Plasmids Spores, Bacterial Bacteria (microorganisms) bacterium subtilis
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v35_n5_p309_Alice http://hdl.handle.net/20.500.12110/paper_03438651_v35_n5_p309_Alice
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03438651_v35_n5_p309_Alice
record_format	dspace
spelling	paper:paper_03438651_v35_n5_p309_Alice2023-06-08T15:34:30Z DNA supercoiling and osmoresistance in Bacillus subtilis 168 Alice, Alejandro Fabián dna topoisomerase (atp hydrolysing) novobiocin plasmid dna article bacillus subtilis bacterial growth bacterium mutant dna supercoiling enzyme activity nonhuman osmoregulation priority journal Anti-Bacterial Agents Bacillus subtilis Chloroquine Culture Media DNA Gyrase DNA Topoisomerases, Type II DNA, Bacterial DNA, Superhelical Genes, Reporter Novobiocin Osmotic Pressure Plasmids Spores, Bacterial Bacillus subtilis Bacteria (microorganisms) bacterium subtilis The importance of the DNA structure for the expression of the osmotic response (osmotolerance) was investigated in Bacillus subtilis 168. Plasmid pUB110 DNA was used as a reporter of the chromosomal DNA topology, and analyses were performed in chloroquine agarose gels. Plasmidic DNA obtained from cultures in Schaeffer medium (D) taken in those periods in which B. subtilis is able to express osmotolerance (early stationary phase or from germinating spores) or from adapted cultures to hyperosmotic medium (DN) presented a higher level of negative supercoiling than DNA samples from vegetative cultures, normally refractory to induction of osmotolerance. The involvement of the DNA gyrase was investigated through the sensitivity to novobiocin, an antibiotic inhibitor of its activity and the behavior of a gyrB1 mutant strain (RG1). In the wild-type strain, the addition of a sublethal concentration of novobiocin (0.5 μg/ml) to the hyperosmotic medium relaxed DNA and inhibited growth. Moreover, already growing cultures in DN medium and later submitted to the same antibiotic presented a relaxed DNA and stopped growing. The RG1 mutant strain submitted to similar novobiocin treatments displayed normal growth in DN novobiocin medium. These results pointed to the requirement of a highly negative supercoiled DNA structure involving the gyrase activity in osmotic response. Fil:Alice, A.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v35_n5_p309_Alice http://hdl.handle.net/20.500.12110/paper_03438651_v35_n5_p309_Alice
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	dna topoisomerase (atp hydrolysing) novobiocin plasmid dna article bacillus subtilis bacterial growth bacterium mutant dna supercoiling enzyme activity nonhuman osmoregulation priority journal Anti-Bacterial Agents Bacillus subtilis Chloroquine Culture Media DNA Gyrase DNA Topoisomerases, Type II DNA, Bacterial DNA, Superhelical Genes, Reporter Novobiocin Osmotic Pressure Plasmids Spores, Bacterial Bacillus subtilis Bacteria (microorganisms) bacterium subtilis
spellingShingle	dna topoisomerase (atp hydrolysing) novobiocin plasmid dna article bacillus subtilis bacterial growth bacterium mutant dna supercoiling enzyme activity nonhuman osmoregulation priority journal Anti-Bacterial Agents Bacillus subtilis Chloroquine Culture Media DNA Gyrase DNA Topoisomerases, Type II DNA, Bacterial DNA, Superhelical Genes, Reporter Novobiocin Osmotic Pressure Plasmids Spores, Bacterial Bacillus subtilis Bacteria (microorganisms) bacterium subtilis Alice, Alejandro Fabián DNA supercoiling and osmoresistance in Bacillus subtilis 168
topic_facet	dna topoisomerase (atp hydrolysing) novobiocin plasmid dna article bacillus subtilis bacterial growth bacterium mutant dna supercoiling enzyme activity nonhuman osmoregulation priority journal Anti-Bacterial Agents Bacillus subtilis Chloroquine Culture Media DNA Gyrase DNA Topoisomerases, Type II DNA, Bacterial DNA, Superhelical Genes, Reporter Novobiocin Osmotic Pressure Plasmids Spores, Bacterial Bacillus subtilis Bacteria (microorganisms) bacterium subtilis
description	The importance of the DNA structure for the expression of the osmotic response (osmotolerance) was investigated in Bacillus subtilis 168. Plasmid pUB110 DNA was used as a reporter of the chromosomal DNA topology, and analyses were performed in chloroquine agarose gels. Plasmidic DNA obtained from cultures in Schaeffer medium (D) taken in those periods in which B. subtilis is able to express osmotolerance (early stationary phase or from germinating spores) or from adapted cultures to hyperosmotic medium (DN) presented a higher level of negative supercoiling than DNA samples from vegetative cultures, normally refractory to induction of osmotolerance. The involvement of the DNA gyrase was investigated through the sensitivity to novobiocin, an antibiotic inhibitor of its activity and the behavior of a gyrB1 mutant strain (RG1). In the wild-type strain, the addition of a sublethal concentration of novobiocin (0.5 μg/ml) to the hyperosmotic medium relaxed DNA and inhibited growth. Moreover, already growing cultures in DN medium and later submitted to the same antibiotic presented a relaxed DNA and stopped growing. The RG1 mutant strain submitted to similar novobiocin treatments displayed normal growth in DN novobiocin medium. These results pointed to the requirement of a highly negative supercoiled DNA structure involving the gyrase activity in osmotic response.
author	Alice, Alejandro Fabián
author_facet	Alice, Alejandro Fabián
author_sort	Alice, Alejandro Fabián
title	DNA supercoiling and osmoresistance in Bacillus subtilis 168
title_short	DNA supercoiling and osmoresistance in Bacillus subtilis 168
title_full	DNA supercoiling and osmoresistance in Bacillus subtilis 168
title_fullStr	DNA supercoiling and osmoresistance in Bacillus subtilis 168
title_full_unstemmed	DNA supercoiling and osmoresistance in Bacillus subtilis 168
title_sort	dna supercoiling and osmoresistance in bacillus subtilis 168
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03438651_v35_n5_p309_Alice http://hdl.handle.net/20.500.12110/paper_03438651_v35_n5_p309_Alice
work_keys_str_mv	AT alicealejandrofabian dnasupercoilingandosmoresistanceinbacillussubtilis168
_version_	1768546302392532992

DNA supercoiling and osmoresistance in Bacillus subtilis 168

Ejemplares similares