Low anticoagulant activity of high sulphated heparan sulphates
Two high sulphated heparin-like polysaccharides (L1, MW 16,000 and L2, MW 11,700) were isolated from rat liver tissues, after DEAE-cellulose chromatography. Heparan sulphates from heart and lung tissues were isolated for comparison and fractionated according to their molecular weight. The anticoagul...
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1990
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v63_n3_p488_Kovensky http://hdl.handle.net/20.500.12110/paper_03406245_v63_n3_p488_Kovensky |
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paper:paper_03406245_v63_n3_p488_Kovensky2023-06-08T15:34:07Z Low anticoagulant activity of high sulphated heparan sulphates Kovensky, José Eduardo Sassetti, Beatriz Fernández Cirelli, Alicia Kordich, Lucía Clelia anticoagulant agent heparan sulfate heparin proteoglycan article gel chromatography human priority journal rat Animal Anticoagulants Glycosaminoglycans Heparan Sulfate Proteoglycan Heparitin Sulfate Liver Proteochondroitin Sulfates Rats Support, Non-U.S. Gov't Two high sulphated heparin-like polysaccharides (L1, MW 16,000 and L2, MW 11,700) were isolated from rat liver tissues, after DEAE-cellulose chromatography. Heparan sulphates from heart and lung tissues were isolated for comparison and fractionated according to their molecular weight. The anticoagulant activities in vitro were studied using clotting antifactor Xa, antifactor IIa, and APTT assay methods, falling in a narrow range (5-44 IU/mg) although the wide variability in molecular weight and sulphate content. The heparan sulphate nature of fractions L1 and L2 (sulphate/disaccharide ratio 2.05 and 2.48, respectively) has been verified by: a) low iduronic/glucuronic acid ratio; b) nitrous acid degradataion followed by gel chromatography; c) heparinase treatment followed by gel chromatography; d) electrophoretic behaviour. Native proteoglycans have been isolated and the glycosidic chains compared with L1 and L2. Their anticoagulant activities in vitro and the fact that antiXa clotting activity was not neutralized by protamine sulphate are in accordance with the results of structural studies. Fil:Kovensky, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sassetti, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernandez Cirelli, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kordich, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v63_n3_p488_Kovensky http://hdl.handle.net/20.500.12110/paper_03406245_v63_n3_p488_Kovensky |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
anticoagulant agent heparan sulfate heparin proteoglycan article gel chromatography human priority journal rat Animal Anticoagulants Glycosaminoglycans Heparan Sulfate Proteoglycan Heparitin Sulfate Liver Proteochondroitin Sulfates Rats Support, Non-U.S. Gov't |
spellingShingle |
anticoagulant agent heparan sulfate heparin proteoglycan article gel chromatography human priority journal rat Animal Anticoagulants Glycosaminoglycans Heparan Sulfate Proteoglycan Heparitin Sulfate Liver Proteochondroitin Sulfates Rats Support, Non-U.S. Gov't Kovensky, José Eduardo Sassetti, Beatriz Fernández Cirelli, Alicia Kordich, Lucía Clelia Low anticoagulant activity of high sulphated heparan sulphates |
topic_facet |
anticoagulant agent heparan sulfate heparin proteoglycan article gel chromatography human priority journal rat Animal Anticoagulants Glycosaminoglycans Heparan Sulfate Proteoglycan Heparitin Sulfate Liver Proteochondroitin Sulfates Rats Support, Non-U.S. Gov't |
description |
Two high sulphated heparin-like polysaccharides (L1, MW 16,000 and L2, MW 11,700) were isolated from rat liver tissues, after DEAE-cellulose chromatography. Heparan sulphates from heart and lung tissues were isolated for comparison and fractionated according to their molecular weight. The anticoagulant activities in vitro were studied using clotting antifactor Xa, antifactor IIa, and APTT assay methods, falling in a narrow range (5-44 IU/mg) although the wide variability in molecular weight and sulphate content. The heparan sulphate nature of fractions L1 and L2 (sulphate/disaccharide ratio 2.05 and 2.48, respectively) has been verified by: a) low iduronic/glucuronic acid ratio; b) nitrous acid degradataion followed by gel chromatography; c) heparinase treatment followed by gel chromatography; d) electrophoretic behaviour. Native proteoglycans have been isolated and the glycosidic chains compared with L1 and L2. Their anticoagulant activities in vitro and the fact that antiXa clotting activity was not neutralized by protamine sulphate are in accordance with the results of structural studies. |
author |
Kovensky, José Eduardo Sassetti, Beatriz Fernández Cirelli, Alicia Kordich, Lucía Clelia |
author_facet |
Kovensky, José Eduardo Sassetti, Beatriz Fernández Cirelli, Alicia Kordich, Lucía Clelia |
author_sort |
Kovensky, José Eduardo |
title |
Low anticoagulant activity of high sulphated heparan sulphates |
title_short |
Low anticoagulant activity of high sulphated heparan sulphates |
title_full |
Low anticoagulant activity of high sulphated heparan sulphates |
title_fullStr |
Low anticoagulant activity of high sulphated heparan sulphates |
title_full_unstemmed |
Low anticoagulant activity of high sulphated heparan sulphates |
title_sort |
low anticoagulant activity of high sulphated heparan sulphates |
publishDate |
1990 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v63_n3_p488_Kovensky http://hdl.handle.net/20.500.12110/paper_03406245_v63_n3_p488_Kovensky |
work_keys_str_mv |
AT kovenskyjoseeduardo lowanticoagulantactivityofhighsulphatedheparansulphates AT sassettibeatriz lowanticoagulantactivityofhighsulphatedheparansulphates AT fernandezcirellialicia lowanticoagulantactivityofhighsulphatedheparansulphates AT kordichluciaclelia lowanticoagulantactivityofhighsulphatedheparansulphates |
_version_ |
1768541895209779200 |