Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation
Carbon monoxide (CO) is a gaseous autacoid known to positively regulate vascular tone; however, its role in angiogenesis is unknown. The aim of this study was to investigate the effect of CO on angiogenesis and vascular endothelial growth factor (VEGF) receptor-2 phosphorylation. Human umbilical vei...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v113_n2_p329_Ahmad http://hdl.handle.net/20.500.12110/paper_03406245_v113_n2_p329_Ahmad |
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paper:paper_03406245_v113_n2_p329_Ahmad2023-06-08T15:34:07Z Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation Angiogenesis Carbon monoxide Endothelial cells Vascular endothelial growth factor Vascular endothelial growth factor receptor-2 actin carbon monoxide cytochrome c oxidase fibroblast growth factor 2 protein kinase B protein tyrosine phosphatase tyrosine vasculotropin receptor 2 carbon monoxide collagen drug combination laminin matrigel organometallic compound proteoglycan recombinant protein tricarbonyldichlororuthenium (II) dimer tyrosine vascular endothelial growth factor receptor-2, human vasculotropin receptor 2 angiogenesis angiogenesis assay animal experiment animal tissue Article cancer inhibition cell migration assay cell proliferation assay controlled study down regulation drug mechanism human human cell immunoprecipitation mouse nonhuman priority journal protein phosphorylation umbilical vein endothelial cell Western blotting animal C57BL mouse cell motion cell proliferation chemistry cytology drug combination endothelium cell metabolism neovascularization (pathology) phosphorylation Animals Carbon Monoxide Cell Movement Cell Proliferation Collagen Drug Combinations Endothelial Cells Human Umbilical Vein Endothelial Cells Humans Immunoprecipitation Laminin Mice, Inbred C57BL Neovascularization, Pathologic Organometallic Compounds Phosphorylation Proteoglycans Recombinant Proteins Tyrosine Vascular Endothelial Growth Factor Receptor-2 Carbon monoxide (CO) is a gaseous autacoid known to positively regulate vascular tone; however, its role in angiogenesis is unknown. The aim of this study was to investigate the effect of CO on angiogenesis and vascular endothelial growth factor (VEGF) receptor-2 phosphorylation. Human umbilical vein endothelial cells (HUVECs) were cultured on growth factor- reduced Matrigel and treated with a CO-releasing molecule (CORM-2) or exposed to CO gas (250 ppm). Here, we report the surprising finding that exposure to CO inhibits vascular endothelial growth factor (VEGF)-induced endothelial cell actin reorganisation, cell proliferation, migration and capillary-like tube formation. Similarly, CO suppressed VEGF-mediated phosphorylation of VEGFR-2 at tyrosine residue 1175 and 1214 and basic fibroblast growth factor- (FGF-2) and VEGF-mediated Akt phosphorylation. Consistent with these data, mice exposed to 250 ppm CO (1h/day for 14 days) exhibited a marked decrease in FGF-2-induced Matrigel plug angiogenesis (p<0.05). These data establish a new biological function for CO in angiogenesis and point to a potential therapeutic use for CO as an anti-angiogenic agent in tumour suppression. © Schattauer 2015. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v113_n2_p329_Ahmad http://hdl.handle.net/20.500.12110/paper_03406245_v113_n2_p329_Ahmad |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Angiogenesis Carbon monoxide Endothelial cells Vascular endothelial growth factor Vascular endothelial growth factor receptor-2 actin carbon monoxide cytochrome c oxidase fibroblast growth factor 2 protein kinase B protein tyrosine phosphatase tyrosine vasculotropin receptor 2 carbon monoxide collagen drug combination laminin matrigel organometallic compound proteoglycan recombinant protein tricarbonyldichlororuthenium (II) dimer tyrosine vascular endothelial growth factor receptor-2, human vasculotropin receptor 2 angiogenesis angiogenesis assay animal experiment animal tissue Article cancer inhibition cell migration assay cell proliferation assay controlled study down regulation drug mechanism human human cell immunoprecipitation mouse nonhuman priority journal protein phosphorylation umbilical vein endothelial cell Western blotting animal C57BL mouse cell motion cell proliferation chemistry cytology drug combination endothelium cell metabolism neovascularization (pathology) phosphorylation Animals Carbon Monoxide Cell Movement Cell Proliferation Collagen Drug Combinations Endothelial Cells Human Umbilical Vein Endothelial Cells Humans Immunoprecipitation Laminin Mice, Inbred C57BL Neovascularization, Pathologic Organometallic Compounds Phosphorylation Proteoglycans Recombinant Proteins Tyrosine Vascular Endothelial Growth Factor Receptor-2 |
| spellingShingle |
Angiogenesis Carbon monoxide Endothelial cells Vascular endothelial growth factor Vascular endothelial growth factor receptor-2 actin carbon monoxide cytochrome c oxidase fibroblast growth factor 2 protein kinase B protein tyrosine phosphatase tyrosine vasculotropin receptor 2 carbon monoxide collagen drug combination laminin matrigel organometallic compound proteoglycan recombinant protein tricarbonyldichlororuthenium (II) dimer tyrosine vascular endothelial growth factor receptor-2, human vasculotropin receptor 2 angiogenesis angiogenesis assay animal experiment animal tissue Article cancer inhibition cell migration assay cell proliferation assay controlled study down regulation drug mechanism human human cell immunoprecipitation mouse nonhuman priority journal protein phosphorylation umbilical vein endothelial cell Western blotting animal C57BL mouse cell motion cell proliferation chemistry cytology drug combination endothelium cell metabolism neovascularization (pathology) phosphorylation Animals Carbon Monoxide Cell Movement Cell Proliferation Collagen Drug Combinations Endothelial Cells Human Umbilical Vein Endothelial Cells Humans Immunoprecipitation Laminin Mice, Inbred C57BL Neovascularization, Pathologic Organometallic Compounds Phosphorylation Proteoglycans Recombinant Proteins Tyrosine Vascular Endothelial Growth Factor Receptor-2 Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation |
| topic_facet |
Angiogenesis Carbon monoxide Endothelial cells Vascular endothelial growth factor Vascular endothelial growth factor receptor-2 actin carbon monoxide cytochrome c oxidase fibroblast growth factor 2 protein kinase B protein tyrosine phosphatase tyrosine vasculotropin receptor 2 carbon monoxide collagen drug combination laminin matrigel organometallic compound proteoglycan recombinant protein tricarbonyldichlororuthenium (II) dimer tyrosine vascular endothelial growth factor receptor-2, human vasculotropin receptor 2 angiogenesis angiogenesis assay animal experiment animal tissue Article cancer inhibition cell migration assay cell proliferation assay controlled study down regulation drug mechanism human human cell immunoprecipitation mouse nonhuman priority journal protein phosphorylation umbilical vein endothelial cell Western blotting animal C57BL mouse cell motion cell proliferation chemistry cytology drug combination endothelium cell metabolism neovascularization (pathology) phosphorylation Animals Carbon Monoxide Cell Movement Cell Proliferation Collagen Drug Combinations Endothelial Cells Human Umbilical Vein Endothelial Cells Humans Immunoprecipitation Laminin Mice, Inbred C57BL Neovascularization, Pathologic Organometallic Compounds Phosphorylation Proteoglycans Recombinant Proteins Tyrosine Vascular Endothelial Growth Factor Receptor-2 |
| description |
Carbon monoxide (CO) is a gaseous autacoid known to positively regulate vascular tone; however, its role in angiogenesis is unknown. The aim of this study was to investigate the effect of CO on angiogenesis and vascular endothelial growth factor (VEGF) receptor-2 phosphorylation. Human umbilical vein endothelial cells (HUVECs) were cultured on growth factor- reduced Matrigel and treated with a CO-releasing molecule (CORM-2) or exposed to CO gas (250 ppm). Here, we report the surprising finding that exposure to CO inhibits vascular endothelial growth factor (VEGF)-induced endothelial cell actin reorganisation, cell proliferation, migration and capillary-like tube formation. Similarly, CO suppressed VEGF-mediated phosphorylation of VEGFR-2 at tyrosine residue 1175 and 1214 and basic fibroblast growth factor- (FGF-2) and VEGF-mediated Akt phosphorylation. Consistent with these data, mice exposed to 250 ppm CO (1h/day for 14 days) exhibited a marked decrease in FGF-2-induced Matrigel plug angiogenesis (p<0.05). These data establish a new biological function for CO in angiogenesis and point to a potential therapeutic use for CO as an anti-angiogenic agent in tumour suppression. © Schattauer 2015. |
| title |
Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation |
| title_short |
Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation |
| title_full |
Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation |
| title_fullStr |
Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation |
| title_full_unstemmed |
Carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation |
| title_sort |
carbon monoxide inhibits sprouting angiogenesis and vascular endothelial growth factor receptor-2 phosphorylation |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03406245_v113_n2_p329_Ahmad http://hdl.handle.net/20.500.12110/paper_03406245_v113_n2_p329_Ahmad |
| _version_ |
1768544366537736192 |