Immunohistochemical localization of vascular endothelial growth factor and its receptor Flk-1 in the amphibian developing principal and accessory olfactory system
In the last years several studies have shown that vascular endothelial growth factor (VEGF) is present in neural stem cells and mature neurons from different neural tissues where it may play an important role as a neuroproliferative and/or antiapoptotic factor. The olfactory neuroepithelium has the...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03402061_v211_n5_p549_Pozzi http://hdl.handle.net/20.500.12110/paper_03402061_v211_n5_p549_Pozzi |
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paper:paper_03402061_v211_n5_p549_Pozzi2023-06-08T15:33:52Z Immunohistochemical localization of vascular endothelial growth factor and its receptor Flk-1 in the amphibian developing principal and accessory olfactory system Pozzi, Andrea Gabriela Yovanovich, Carola Anabel Mariana Jungblut, Lucas David Paz, Dante Agustin Flk-1 Neural differentiation Olfactory epithelium Toad VEGF vasculotropin vasculotropin receptor 2 Amphibia animal cell animal experiment animal tissue article basement membrane Bowman membrane cell labeling cell maturation cell membrane cell nucleus cytoplasm embryo embryo development epithelium cell immunofluorescence immunohistochemistry immunoreactivity larval stage metamorphosis nerve cell differentiation nonhuman olfactory bulb olfactory epithelium olfactory nerve olfactory system priority journal protein expression protein localization toad Amphibia Anura Bufo arenarum In the last years several studies have shown that vascular endothelial growth factor (VEGF) is present in neural stem cells and mature neurons from different neural tissues where it may play an important role as a neuroproliferative and/or antiapoptotic factor. The olfactory neuroepithelium has the capability to replace dying neurons with new neurons formed by cell division from stem cells in the basal region of the epithelium. The present study demonstrates, for the first time, that VEGF is present in the olfactory epithelium, nerves and bulbs (both main and accessory) during the development of the toad Bufo arenarum. In this report, we detected VEGF immunoreactivity in mature olfactory neurons from early larval stages until the beginning of the metamorphic climax. VEGF expression decreases dramatically after metamorphosis. VEGF receptor Flk-1 was localized by immunohistochemistry, from premetamorphic larval stages until the climax in the neurons of the olfactory epithelium with a more intense labeling in the basal cell layer. Double-label immunofluorescence studies localized VEGF to the cytoplasm and the nucleus of mature neurons whereas Flk-1 was expressed in cell membranes. Flk-1 was present in neurons of both the main and accessory olfactory bulbs. After the end of metamorphosis, Flk-1 expression was limited to basal cells in the olfactory epithelium and Bowman's glands. The main and accessory olfactory bulbs showed the same pattern of Flk-1 immunostaining before and after the end of metamorphosis. The presence of VEGF and its receptor in the olfactory system suggests that VEGF may play an important role during neural development. © 2006 Springer-Verlag. Fil:Pozzi, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Yovanovich, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jungblut, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03402061_v211_n5_p549_Pozzi http://hdl.handle.net/20.500.12110/paper_03402061_v211_n5_p549_Pozzi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Flk-1 Neural differentiation Olfactory epithelium Toad VEGF vasculotropin vasculotropin receptor 2 Amphibia animal cell animal experiment animal tissue article basement membrane Bowman membrane cell labeling cell maturation cell membrane cell nucleus cytoplasm embryo embryo development epithelium cell immunofluorescence immunohistochemistry immunoreactivity larval stage metamorphosis nerve cell differentiation nonhuman olfactory bulb olfactory epithelium olfactory nerve olfactory system priority journal protein expression protein localization toad Amphibia Anura Bufo arenarum |
| spellingShingle |
Flk-1 Neural differentiation Olfactory epithelium Toad VEGF vasculotropin vasculotropin receptor 2 Amphibia animal cell animal experiment animal tissue article basement membrane Bowman membrane cell labeling cell maturation cell membrane cell nucleus cytoplasm embryo embryo development epithelium cell immunofluorescence immunohistochemistry immunoreactivity larval stage metamorphosis nerve cell differentiation nonhuman olfactory bulb olfactory epithelium olfactory nerve olfactory system priority journal protein expression protein localization toad Amphibia Anura Bufo arenarum Pozzi, Andrea Gabriela Yovanovich, Carola Anabel Mariana Jungblut, Lucas David Paz, Dante Agustin Immunohistochemical localization of vascular endothelial growth factor and its receptor Flk-1 in the amphibian developing principal and accessory olfactory system |
| topic_facet |
Flk-1 Neural differentiation Olfactory epithelium Toad VEGF vasculotropin vasculotropin receptor 2 Amphibia animal cell animal experiment animal tissue article basement membrane Bowman membrane cell labeling cell maturation cell membrane cell nucleus cytoplasm embryo embryo development epithelium cell immunofluorescence immunohistochemistry immunoreactivity larval stage metamorphosis nerve cell differentiation nonhuman olfactory bulb olfactory epithelium olfactory nerve olfactory system priority journal protein expression protein localization toad Amphibia Anura Bufo arenarum |
| description |
In the last years several studies have shown that vascular endothelial growth factor (VEGF) is present in neural stem cells and mature neurons from different neural tissues where it may play an important role as a neuroproliferative and/or antiapoptotic factor. The olfactory neuroepithelium has the capability to replace dying neurons with new neurons formed by cell division from stem cells in the basal region of the epithelium. The present study demonstrates, for the first time, that VEGF is present in the olfactory epithelium, nerves and bulbs (both main and accessory) during the development of the toad Bufo arenarum. In this report, we detected VEGF immunoreactivity in mature olfactory neurons from early larval stages until the beginning of the metamorphic climax. VEGF expression decreases dramatically after metamorphosis. VEGF receptor Flk-1 was localized by immunohistochemistry, from premetamorphic larval stages until the climax in the neurons of the olfactory epithelium with a more intense labeling in the basal cell layer. Double-label immunofluorescence studies localized VEGF to the cytoplasm and the nucleus of mature neurons whereas Flk-1 was expressed in cell membranes. Flk-1 was present in neurons of both the main and accessory olfactory bulbs. After the end of metamorphosis, Flk-1 expression was limited to basal cells in the olfactory epithelium and Bowman's glands. The main and accessory olfactory bulbs showed the same pattern of Flk-1 immunostaining before and after the end of metamorphosis. The presence of VEGF and its receptor in the olfactory system suggests that VEGF may play an important role during neural development. © 2006 Springer-Verlag. |
| author |
Pozzi, Andrea Gabriela Yovanovich, Carola Anabel Mariana Jungblut, Lucas David Paz, Dante Agustin |
| author_facet |
Pozzi, Andrea Gabriela Yovanovich, Carola Anabel Mariana Jungblut, Lucas David Paz, Dante Agustin |
| author_sort |
Pozzi, Andrea Gabriela |
| title |
Immunohistochemical localization of vascular endothelial growth factor and its receptor Flk-1 in the amphibian developing principal and accessory olfactory system |
| title_short |
Immunohistochemical localization of vascular endothelial growth factor and its receptor Flk-1 in the amphibian developing principal and accessory olfactory system |
| title_full |
Immunohistochemical localization of vascular endothelial growth factor and its receptor Flk-1 in the amphibian developing principal and accessory olfactory system |
| title_fullStr |
Immunohistochemical localization of vascular endothelial growth factor and its receptor Flk-1 in the amphibian developing principal and accessory olfactory system |
| title_full_unstemmed |
Immunohistochemical localization of vascular endothelial growth factor and its receptor Flk-1 in the amphibian developing principal and accessory olfactory system |
| title_sort |
immunohistochemical localization of vascular endothelial growth factor and its receptor flk-1 in the amphibian developing principal and accessory olfactory system |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03402061_v211_n5_p549_Pozzi http://hdl.handle.net/20.500.12110/paper_03402061_v211_n5_p549_Pozzi |
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| _version_ |
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