Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation
Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) expressing cells were detected in pituitary, brain and ovary of the Perciform cichlid fish Cichlasoma dimerus. This detection was carried out by immunohistochemistry (IHC) and Western blot techniques using antisera of the Cyprinodontifo...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03402061_v211_n5_p355_Pandolfi http://hdl.handle.net/20.500.12110/paper_03402061_v211_n5_p355_Pandolfi |
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paper:paper_03402061_v211_n5_p355_Pandolfi2023-06-08T15:33:52Z Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation Pandolfi, Matías Lo Nostro, Fabiana Laura Pozzi, Andrea Gabriela Meijide, Fernando Javier Maggese, María Cristina Irene Cichlidae Gonadotropins Ontogeny Sexual differentiation Teleostei antiserum follitropin luteinizing hormone animal cell article brain cell type cichlid controlled study Cyprinodontiformes cytoplasm female follitropin release hypophysis hypophysis intermediate lobe hypophysis pars distalis hypothalamus hypothalamus nucleus immunocompetent cell immunohistochemistry luteinizing hormone release male molecular weight nerve cell neurohypophysis nonhuman ontogeny organ culture ovary preoptic area priority journal sex differentiation teleost vitellogenesis Western blotting Age Factors Animals Brain Cichlids Female Follicle Stimulating Hormone Gonadotropin-Releasing Hormone Immunohistochemistry Luteinizing Hormone Male Nerve Fibers Organ Culture Techniques Ovary Pituitary Gland Sex Differentiation Testis Vitellogenesis Cichlasoma Cichlidae Cyprinodontiformes Fundulus heteroclitus Perciformes Teleostei Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) expressing cells were detected in pituitary, brain and ovary of the Perciform cichlid fish Cichlasoma dimerus. This detection was carried out by immunohistochemistry (IHC) and Western blot techniques using antisera of the Cyprinodontiform Fundulus heteroclitus raised against the conservative region of the teleost βFSH and the βLH subunits. The estimated molecular weights were 24 kDa for LH and 19 and 15 kDa for FSH. In the adult pituitary, both cell types were distributed along mid and ventral zones of the proximal pars distalis (PPD, mid-immunoreactive cells), and along the ventral and dorsal external border of the pars intermedia (PI, high-immunoreactive cells). Double IHC showed that FSH and LH are mainly expressed in different pituitary cells. FSH cells were detected in the pituitary around day 21 after hatching (ah) (prior to sex differentiation), while LH cells were detected by day 60 ah (during the sexual differentiation period). A correlation between gonadal sex differentiation and FSH was demonstrated in a 15 days organ culture system. FSH and LH neurons were localized in the nucleus lateralis tuberis and their fibers project through the ventral hypothalamus, preoptic area and neurohypophysis. FSH neurons differentiated on day 21 ah, while LH neurons appeared on day 15 ah. In the ovary, the immunoreactivity for both FSH and LH was restricted to the cytoplasm of previtellogenic and early vitellogenic oocytes. © 2006 Springer-Verlag. Fil:Pandolfi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lo Nostro, F.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pozzi, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Meijide, F.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maggese, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03402061_v211_n5_p355_Pandolfi http://hdl.handle.net/20.500.12110/paper_03402061_v211_n5_p355_Pandolfi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cichlidae Gonadotropins Ontogeny Sexual differentiation Teleostei antiserum follitropin luteinizing hormone animal cell article brain cell type cichlid controlled study Cyprinodontiformes cytoplasm female follitropin release hypophysis hypophysis intermediate lobe hypophysis pars distalis hypothalamus hypothalamus nucleus immunocompetent cell immunohistochemistry luteinizing hormone release male molecular weight nerve cell neurohypophysis nonhuman ontogeny organ culture ovary preoptic area priority journal sex differentiation teleost vitellogenesis Western blotting Age Factors Animals Brain Cichlids Female Follicle Stimulating Hormone Gonadotropin-Releasing Hormone Immunohistochemistry Luteinizing Hormone Male Nerve Fibers Organ Culture Techniques Ovary Pituitary Gland Sex Differentiation Testis Vitellogenesis Cichlasoma Cichlidae Cyprinodontiformes Fundulus heteroclitus Perciformes Teleostei |
| spellingShingle |
Cichlidae Gonadotropins Ontogeny Sexual differentiation Teleostei antiserum follitropin luteinizing hormone animal cell article brain cell type cichlid controlled study Cyprinodontiformes cytoplasm female follitropin release hypophysis hypophysis intermediate lobe hypophysis pars distalis hypothalamus hypothalamus nucleus immunocompetent cell immunohistochemistry luteinizing hormone release male molecular weight nerve cell neurohypophysis nonhuman ontogeny organ culture ovary preoptic area priority journal sex differentiation teleost vitellogenesis Western blotting Age Factors Animals Brain Cichlids Female Follicle Stimulating Hormone Gonadotropin-Releasing Hormone Immunohistochemistry Luteinizing Hormone Male Nerve Fibers Organ Culture Techniques Ovary Pituitary Gland Sex Differentiation Testis Vitellogenesis Cichlasoma Cichlidae Cyprinodontiformes Fundulus heteroclitus Perciformes Teleostei Pandolfi, Matías Lo Nostro, Fabiana Laura Pozzi, Andrea Gabriela Meijide, Fernando Javier Maggese, María Cristina Irene Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation |
| topic_facet |
Cichlidae Gonadotropins Ontogeny Sexual differentiation Teleostei antiserum follitropin luteinizing hormone animal cell article brain cell type cichlid controlled study Cyprinodontiformes cytoplasm female follitropin release hypophysis hypophysis intermediate lobe hypophysis pars distalis hypothalamus hypothalamus nucleus immunocompetent cell immunohistochemistry luteinizing hormone release male molecular weight nerve cell neurohypophysis nonhuman ontogeny organ culture ovary preoptic area priority journal sex differentiation teleost vitellogenesis Western blotting Age Factors Animals Brain Cichlids Female Follicle Stimulating Hormone Gonadotropin-Releasing Hormone Immunohistochemistry Luteinizing Hormone Male Nerve Fibers Organ Culture Techniques Ovary Pituitary Gland Sex Differentiation Testis Vitellogenesis Cichlasoma Cichlidae Cyprinodontiformes Fundulus heteroclitus Perciformes Teleostei |
| description |
Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) expressing cells were detected in pituitary, brain and ovary of the Perciform cichlid fish Cichlasoma dimerus. This detection was carried out by immunohistochemistry (IHC) and Western blot techniques using antisera of the Cyprinodontiform Fundulus heteroclitus raised against the conservative region of the teleost βFSH and the βLH subunits. The estimated molecular weights were 24 kDa for LH and 19 and 15 kDa for FSH. In the adult pituitary, both cell types were distributed along mid and ventral zones of the proximal pars distalis (PPD, mid-immunoreactive cells), and along the ventral and dorsal external border of the pars intermedia (PI, high-immunoreactive cells). Double IHC showed that FSH and LH are mainly expressed in different pituitary cells. FSH cells were detected in the pituitary around day 21 after hatching (ah) (prior to sex differentiation), while LH cells were detected by day 60 ah (during the sexual differentiation period). A correlation between gonadal sex differentiation and FSH was demonstrated in a 15 days organ culture system. FSH and LH neurons were localized in the nucleus lateralis tuberis and their fibers project through the ventral hypothalamus, preoptic area and neurohypophysis. FSH neurons differentiated on day 21 ah, while LH neurons appeared on day 15 ah. In the ovary, the immunoreactivity for both FSH and LH was restricted to the cytoplasm of previtellogenic and early vitellogenic oocytes. © 2006 Springer-Verlag. |
| author |
Pandolfi, Matías Lo Nostro, Fabiana Laura Pozzi, Andrea Gabriela Meijide, Fernando Javier Maggese, María Cristina Irene |
| author_facet |
Pandolfi, Matías Lo Nostro, Fabiana Laura Pozzi, Andrea Gabriela Meijide, Fernando Javier Maggese, María Cristina Irene |
| author_sort |
Pandolfi, Matías |
| title |
Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation |
| title_short |
Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation |
| title_full |
Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation |
| title_fullStr |
Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation |
| title_full_unstemmed |
Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation |
| title_sort |
identification of immunoreactive fsh and lh cells in the cichlid fish cichlasoma dimerus during the ontogeny and sexual differentiation |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03402061_v211_n5_p355_Pandolfi http://hdl.handle.net/20.500.12110/paper_03402061_v211_n5_p355_Pandolfi |
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