A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation
Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correla...
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v32_n1_p1_Capani http://hdl.handle.net/20.500.12110/paper_03279545_v32_n1_p1_Capani |
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paper:paper_03279545_v32_n1_p1_Capani2023-06-08T15:33:43Z A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation 3-D reconstructions Electron tomography F-actin Phalloidin-eosin Phootoxidation eosin F actin phalloidin actin filament cellular distribution central nervous system electron beam tomography fluorescence microscopy nonhuman photooxidation Purkinje cell review three dimensional imaging transmission electron microscopy Actins Animals Central Nervous System Eosine Yellowish-(YS) Fluorescent Dyes Humans Imaging, Three-Dimensional Microfilaments Microscopy, Fluorescence Models, Molecular Oxidation-Reduction Phalloidine Photons Staining and Labeling Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a valuable tool for revealing aspects of the synaptic morphology that are not available when conventional electron microscopy is used. By applying this selective staining technique and three-dimensional imaging, we uncovered the structural organization of actin in the postsynaptic densities in physiological and pathological conditions. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v32_n1_p1_Capani http://hdl.handle.net/20.500.12110/paper_03279545_v32_n1_p1_Capani |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
3-D reconstructions Electron tomography F-actin Phalloidin-eosin Phootoxidation eosin F actin phalloidin actin filament cellular distribution central nervous system electron beam tomography fluorescence microscopy nonhuman photooxidation Purkinje cell review three dimensional imaging transmission electron microscopy Actins Animals Central Nervous System Eosine Yellowish-(YS) Fluorescent Dyes Humans Imaging, Three-Dimensional Microfilaments Microscopy, Fluorescence Models, Molecular Oxidation-Reduction Phalloidine Photons Staining and Labeling |
| spellingShingle |
3-D reconstructions Electron tomography F-actin Phalloidin-eosin Phootoxidation eosin F actin phalloidin actin filament cellular distribution central nervous system electron beam tomography fluorescence microscopy nonhuman photooxidation Purkinje cell review three dimensional imaging transmission electron microscopy Actins Animals Central Nervous System Eosine Yellowish-(YS) Fluorescent Dyes Humans Imaging, Three-Dimensional Microfilaments Microscopy, Fluorescence Models, Molecular Oxidation-Reduction Phalloidine Photons Staining and Labeling A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation |
| topic_facet |
3-D reconstructions Electron tomography F-actin Phalloidin-eosin Phootoxidation eosin F actin phalloidin actin filament cellular distribution central nervous system electron beam tomography fluorescence microscopy nonhuman photooxidation Purkinje cell review three dimensional imaging transmission electron microscopy Actins Animals Central Nervous System Eosine Yellowish-(YS) Fluorescent Dyes Humans Imaging, Three-Dimensional Microfilaments Microscopy, Fluorescence Models, Molecular Oxidation-Reduction Phalloidine Photons Staining and Labeling |
| description |
Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a valuable tool for revealing aspects of the synaptic morphology that are not available when conventional electron microscopy is used. By applying this selective staining technique and three-dimensional imaging, we uncovered the structural organization of actin in the postsynaptic densities in physiological and pathological conditions. |
| title |
A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation |
| title_short |
A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation |
| title_full |
A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation |
| title_fullStr |
A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation |
| title_full_unstemmed |
A tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation |
| title_sort |
tridimensional view of the organization of actin filaments in the central nervous system by use of fluorescent photooxidation |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v32_n1_p1_Capani http://hdl.handle.net/20.500.12110/paper_03279545_v32_n1_p1_Capani |
| _version_ |
1768546253872824320 |