MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2
Prior to this work, we found that adrenal as well as extra-adrenal factors activate the response of renal l 11β-hydroxysteroid dehydrogenase 2 to stressful situations. These results -showing ways through which the organism hinders the pathological occupation of mineralocorticoid receptors by glucoco...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v30_n3_p469_Zallocchi http://hdl.handle.net/20.500.12110/paper_03279545_v30_n3_p469_Zallocchi |
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paper:paper_03279545_v30_n3_p469_Zallocchi2023-06-08T15:33:42Z MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2 Zallocchi, Marisa Laura Calvo, Juan Carlos Matkovic, Laura Beatriz 11β-HSD2 Glucocorticoid Mineralocorticoid Serotonin Stress hormones 11beta hydroxysteroid dehydrogenase 11beta hydroxysteroid dehydrogenase 2 corticosterone dehydrocorticosterone glucocorticoid ketanserin metitepine mineralocorticoid receptor serotonin unclassified drug animal cell article cell strain chemical analysis enzyme activation enzyme activity enzyme analysis enzyme inhibition enzyme substrate epithelium cell high performance liquid chromatography hormone action nephron nonhuman protein expression 11-beta-Hydroxysteroid Dehydrogenase Type 2 Animals Cell Line Corticosterone Dogs Enzyme Activation Nephrons Paracrine Communication Serotonin Prior to this work, we found that adrenal as well as extra-adrenal factors activate the response of renal l 11β-hydroxysteroid dehydrogenase 2 to stressful situations. These results -showing ways through which the organism hinders the pathological occupation of mineralocorticoid receptors by glucocorticoids leading to sodium retention and hypertension- prompted the present study on the nature of the above-mentioned extra-adrenal factors. Serotonin was chosen because of its properties as a widely distributed neurohormone, known to interact with glucocorticoids at many sites, also exhibiting increased levels and effects under stressful situations. We studied serotonin effects on 11β-hydroxysteroid dehydrogenase 2 activity in a cell line derived from distal nephron polarized-epithelium, employing 3H-corticosterone as substrate. The end-product, 3H-11- dehydrocorticosterone was separated from the substrate by HPLC and quantified. Serotonin stimulated 11β-hydroxysteroid dehydrogenase 2 activity only at 2nM and 25pM, the magnitude of the response depending also on substrate concentration. The stimulation was blocked by the specific inhibitors methiothepin and ketanserin. We postulate that the organism partially prevents renal mineralocorticoid receptor occupancy by glucocorticoids, circulating at enhanced levels under stressful situations, through serotonin-mediated catabolic regulation of the 11β-hydroxysteroid dehydrogenase 2 activity. Given many, mostly positive, interactions between both hormones, this might eventually pave the way to studies on a new regulatory axis. Fil:Zallocchi, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matkovic', L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v30_n3_p469_Zallocchi http://hdl.handle.net/20.500.12110/paper_03279545_v30_n3_p469_Zallocchi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
11β-HSD2 Glucocorticoid Mineralocorticoid Serotonin Stress hormones 11beta hydroxysteroid dehydrogenase 11beta hydroxysteroid dehydrogenase 2 corticosterone dehydrocorticosterone glucocorticoid ketanserin metitepine mineralocorticoid receptor serotonin unclassified drug animal cell article cell strain chemical analysis enzyme activation enzyme activity enzyme analysis enzyme inhibition enzyme substrate epithelium cell high performance liquid chromatography hormone action nephron nonhuman protein expression 11-beta-Hydroxysteroid Dehydrogenase Type 2 Animals Cell Line Corticosterone Dogs Enzyme Activation Nephrons Paracrine Communication Serotonin |
| spellingShingle |
11β-HSD2 Glucocorticoid Mineralocorticoid Serotonin Stress hormones 11beta hydroxysteroid dehydrogenase 11beta hydroxysteroid dehydrogenase 2 corticosterone dehydrocorticosterone glucocorticoid ketanserin metitepine mineralocorticoid receptor serotonin unclassified drug animal cell article cell strain chemical analysis enzyme activation enzyme activity enzyme analysis enzyme inhibition enzyme substrate epithelium cell high performance liquid chromatography hormone action nephron nonhuman protein expression 11-beta-Hydroxysteroid Dehydrogenase Type 2 Animals Cell Line Corticosterone Dogs Enzyme Activation Nephrons Paracrine Communication Serotonin Zallocchi, Marisa Laura Calvo, Juan Carlos Matkovic, Laura Beatriz MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2 |
| topic_facet |
11β-HSD2 Glucocorticoid Mineralocorticoid Serotonin Stress hormones 11beta hydroxysteroid dehydrogenase 11beta hydroxysteroid dehydrogenase 2 corticosterone dehydrocorticosterone glucocorticoid ketanserin metitepine mineralocorticoid receptor serotonin unclassified drug animal cell article cell strain chemical analysis enzyme activation enzyme activity enzyme analysis enzyme inhibition enzyme substrate epithelium cell high performance liquid chromatography hormone action nephron nonhuman protein expression 11-beta-Hydroxysteroid Dehydrogenase Type 2 Animals Cell Line Corticosterone Dogs Enzyme Activation Nephrons Paracrine Communication Serotonin |
| description |
Prior to this work, we found that adrenal as well as extra-adrenal factors activate the response of renal l 11β-hydroxysteroid dehydrogenase 2 to stressful situations. These results -showing ways through which the organism hinders the pathological occupation of mineralocorticoid receptors by glucocorticoids leading to sodium retention and hypertension- prompted the present study on the nature of the above-mentioned extra-adrenal factors. Serotonin was chosen because of its properties as a widely distributed neurohormone, known to interact with glucocorticoids at many sites, also exhibiting increased levels and effects under stressful situations. We studied serotonin effects on 11β-hydroxysteroid dehydrogenase 2 activity in a cell line derived from distal nephron polarized-epithelium, employing 3H-corticosterone as substrate. The end-product, 3H-11- dehydrocorticosterone was separated from the substrate by HPLC and quantified. Serotonin stimulated 11β-hydroxysteroid dehydrogenase 2 activity only at 2nM and 25pM, the magnitude of the response depending also on substrate concentration. The stimulation was blocked by the specific inhibitors methiothepin and ketanserin. We postulate that the organism partially prevents renal mineralocorticoid receptor occupancy by glucocorticoids, circulating at enhanced levels under stressful situations, through serotonin-mediated catabolic regulation of the 11β-hydroxysteroid dehydrogenase 2 activity. Given many, mostly positive, interactions between both hormones, this might eventually pave the way to studies on a new regulatory axis. |
| author |
Zallocchi, Marisa Laura Calvo, Juan Carlos Matkovic, Laura Beatriz |
| author_facet |
Zallocchi, Marisa Laura Calvo, Juan Carlos Matkovic, Laura Beatriz |
| author_sort |
Zallocchi, Marisa Laura |
| title |
MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2 |
| title_short |
MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2 |
| title_full |
MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2 |
| title_fullStr |
MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2 |
| title_full_unstemmed |
MDCK cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2 |
| title_sort |
mdck cells express serotonin-regulable 11β-hydroxysteroid dehydrogenase type 2 |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v30_n3_p469_Zallocchi http://hdl.handle.net/20.500.12110/paper_03279545_v30_n3_p469_Zallocchi |
| work_keys_str_mv |
AT zallocchimarisalaura mdckcellsexpressserotoninregulable11bhydroxysteroiddehydrogenasetype2 AT calvojuancarlos mdckcellsexpressserotoninregulable11bhydroxysteroiddehydrogenasetype2 AT matkoviclaurabeatriz mdckcellsexpressserotoninregulable11bhydroxysteroiddehydrogenasetype2 |
| _version_ |
1768545556701904896 |