Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase
The localization and subcellular distribution of Trypanosoma cruzi nitric oxide synthase was investigated in epimastigote cells by immunocytochemistry at electron and light microscope level, using a polyclonal antibody to neuronal nitric oxide synthase, and also, at light microscope level, by the ni...
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2000
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v24_n3_p217_Goldstein http://hdl.handle.net/20.500.12110/paper_03279545_v24_n3_p217_Goldstein |
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paper:paper_03279545_v24_n3_p217_Goldstein2023-06-08T15:33:39Z Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase Diaphorase Immunocytochemistry Nitric oxide synthase Subcellular localization Trypanosoma cruzi formazan nitric oxide synthase reduced nicotinamide adenine dinucleotide phosphate dehydrogenase animal cell apical membrane article cell membrane cell motility cell ultrastructure cellular distribution enzyme localization epimastigote flagellum immunocytochemistry nonhuman parasite migration signal transduction Trypanosoma cruzi Animals Cell Compartmentation Cell Membrane Cell Movement Flagella Nitric Oxide Nitric Oxide Synthase Signal Transduction Trypanosoma cruzi Animalia Trypanosoma Trypanosoma cruzi The localization and subcellular distribution of Trypanosoma cruzi nitric oxide synthase was investigated in epimastigote cells by immunocytochemistry at electron and light microscope level, using a polyclonal antibody to neuronal nitric oxide synthase, and also, at light microscope level, by the nicotinamide adenine dinucleotide phosphate-diaphorase histochemical reaction. The immunoreactivity was ultrastructurally localized by electron microscopy in the inner surface of cell membranes and in free cytosolic clusters in the body, flagellum and apical extreme. Light microscopy showed that immunoprecipitates, specific for the Trypanosoma cruzi nitric oxide synthase, co-localized with the formazan precipitates generated by the diaphorase reaction in the same areas identified by electron microscopy. These results, taken together with previous finding from our laboratory could help to explain the involvement of the nitric oxide transduction pathway in T. cruzi epimastigote motility. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v24_n3_p217_Goldstein http://hdl.handle.net/20.500.12110/paper_03279545_v24_n3_p217_Goldstein |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Diaphorase Immunocytochemistry Nitric oxide synthase Subcellular localization Trypanosoma cruzi formazan nitric oxide synthase reduced nicotinamide adenine dinucleotide phosphate dehydrogenase animal cell apical membrane article cell membrane cell motility cell ultrastructure cellular distribution enzyme localization epimastigote flagellum immunocytochemistry nonhuman parasite migration signal transduction Trypanosoma cruzi Animals Cell Compartmentation Cell Membrane Cell Movement Flagella Nitric Oxide Nitric Oxide Synthase Signal Transduction Trypanosoma cruzi Animalia Trypanosoma Trypanosoma cruzi |
| spellingShingle |
Diaphorase Immunocytochemistry Nitric oxide synthase Subcellular localization Trypanosoma cruzi formazan nitric oxide synthase reduced nicotinamide adenine dinucleotide phosphate dehydrogenase animal cell apical membrane article cell membrane cell motility cell ultrastructure cellular distribution enzyme localization epimastigote flagellum immunocytochemistry nonhuman parasite migration signal transduction Trypanosoma cruzi Animals Cell Compartmentation Cell Membrane Cell Movement Flagella Nitric Oxide Nitric Oxide Synthase Signal Transduction Trypanosoma cruzi Animalia Trypanosoma Trypanosoma cruzi Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase |
| topic_facet |
Diaphorase Immunocytochemistry Nitric oxide synthase Subcellular localization Trypanosoma cruzi formazan nitric oxide synthase reduced nicotinamide adenine dinucleotide phosphate dehydrogenase animal cell apical membrane article cell membrane cell motility cell ultrastructure cellular distribution enzyme localization epimastigote flagellum immunocytochemistry nonhuman parasite migration signal transduction Trypanosoma cruzi Animals Cell Compartmentation Cell Membrane Cell Movement Flagella Nitric Oxide Nitric Oxide Synthase Signal Transduction Trypanosoma cruzi Animalia Trypanosoma Trypanosoma cruzi |
| description |
The localization and subcellular distribution of Trypanosoma cruzi nitric oxide synthase was investigated in epimastigote cells by immunocytochemistry at electron and light microscope level, using a polyclonal antibody to neuronal nitric oxide synthase, and also, at light microscope level, by the nicotinamide adenine dinucleotide phosphate-diaphorase histochemical reaction. The immunoreactivity was ultrastructurally localized by electron microscopy in the inner surface of cell membranes and in free cytosolic clusters in the body, flagellum and apical extreme. Light microscopy showed that immunoprecipitates, specific for the Trypanosoma cruzi nitric oxide synthase, co-localized with the formazan precipitates generated by the diaphorase reaction in the same areas identified by electron microscopy. These results, taken together with previous finding from our laboratory could help to explain the involvement of the nitric oxide transduction pathway in T. cruzi epimastigote motility. |
| title |
Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase |
| title_short |
Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase |
| title_full |
Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase |
| title_fullStr |
Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase |
| title_full_unstemmed |
Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase |
| title_sort |
immuno and cytochemical localization of trypanosoma cruzi nitric oxide synthase |
| publishDate |
2000 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v24_n3_p217_Goldstein http://hdl.handle.net/20.500.12110/paper_03279545_v24_n3_p217_Goldstein |
| _version_ |
1768545005741277184 |