Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors

In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the m...

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Autores principales:	Gazzaniga, Silvina N., Wainstok de Calmanovici, Rosa
Publicado:	1997
Materias:	Endothelial cells MEC 13.3 Mice mammary tumors Mouse CD31/PECAM monoclonal antibody adenocarcinoma animal antibody specificity antigen antibody reaction article Bagg albino mouse cell culture chemistry cytology experimental neoplasm female immunohistochemistry immunology microcirculation mouse udder vascular endothelium vascularization Adenocarcinoma Animals Antibodies, Monoclonal Antibody Specificity Antigen-Antibody Reactions Endothelium, Vascular Female Immunohistochemistry Mammary Glands, Animal Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Microcirculation Tumor Cells, Cultured
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v21_n1_p39_Vanzulli http://hdl.handle.net/20.500.12110/paper_03279545_v21_n1_p39_Vanzulli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03279545_v21_n1_p39_Vanzulli
record_format	dspace
spelling	paper:paper_03279545_v21_n1_p39_Vanzulli2023-06-08T15:33:36Z Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors Gazzaniga, Silvina N. Wainstok de Calmanovici, Rosa Endothelial cells MEC 13.3 Mice mammary tumors Mouse CD31/PECAM monoclonal antibody adenocarcinoma animal antibody specificity antigen antibody reaction article Bagg albino mouse cell culture chemistry cytology experimental neoplasm female immunohistochemistry immunology microcirculation mouse udder vascular endothelium vascularization Adenocarcinoma Animals Antibodies, Monoclonal Antibody Specificity Antigen-Antibody Reactions Endothelium, Vascular Female Immunohistochemistry Mammary Glands, Animal Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Microcirculation Tumor Cells, Cultured In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the murine form of CD31/PECAM. This mAb with immunoenzymatic technique or inmunofluorescent labelling, was found to be a useful tool to quantify tumor neovascularization. Specifically, membrane reinforcement could be observed in vessel ECs, indicating the expression of CD31/ PECAM in their surface. The staining of ECs from tumors and from normal tissues was also compared. In this work, the use of MEC13.3 mAb is reported to recognize mice mammary tumor ECs as a useful tool to identify neovascularization. It would also be helpful for research on the origin and function of vascular endothelium in murine tumor experimental models. Fil:Gazzaniga, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wainstok De Calmanovici, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v21_n1_p39_Vanzulli http://hdl.handle.net/20.500.12110/paper_03279545_v21_n1_p39_Vanzulli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Endothelial cells MEC 13.3 Mice mammary tumors Mouse CD31/PECAM monoclonal antibody adenocarcinoma animal antibody specificity antigen antibody reaction article Bagg albino mouse cell culture chemistry cytology experimental neoplasm female immunohistochemistry immunology microcirculation mouse udder vascular endothelium vascularization Adenocarcinoma Animals Antibodies, Monoclonal Antibody Specificity Antigen-Antibody Reactions Endothelium, Vascular Female Immunohistochemistry Mammary Glands, Animal Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Microcirculation Tumor Cells, Cultured
spellingShingle	Endothelial cells MEC 13.3 Mice mammary tumors Mouse CD31/PECAM monoclonal antibody adenocarcinoma animal antibody specificity antigen antibody reaction article Bagg albino mouse cell culture chemistry cytology experimental neoplasm female immunohistochemistry immunology microcirculation mouse udder vascular endothelium vascularization Adenocarcinoma Animals Antibodies, Monoclonal Antibody Specificity Antigen-Antibody Reactions Endothelium, Vascular Female Immunohistochemistry Mammary Glands, Animal Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Microcirculation Tumor Cells, Cultured Gazzaniga, Silvina N. Wainstok de Calmanovici, Rosa Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors
topic_facet	Endothelial cells MEC 13.3 Mice mammary tumors Mouse CD31/PECAM monoclonal antibody adenocarcinoma animal antibody specificity antigen antibody reaction article Bagg albino mouse cell culture chemistry cytology experimental neoplasm female immunohistochemistry immunology microcirculation mouse udder vascular endothelium vascularization Adenocarcinoma Animals Antibodies, Monoclonal Antibody Specificity Antigen-Antibody Reactions Endothelium, Vascular Female Immunohistochemistry Mammary Glands, Animal Mammary Neoplasms, Experimental Mice Mice, Inbred BALB C Microcirculation Tumor Cells, Cultured
description	In order to clearly visualize blood vessels, the monoclonal antibody (mAb) MEC 13.3 was used for an immunohistochemical staining on frozen sections of different mice mammary tumors. MEC 13.3 mAb is specific for endothelial cells (ECs) of mouse blood vessels and recognizes a molecule related to the murine form of CD31/PECAM. This mAb with immunoenzymatic technique or inmunofluorescent labelling, was found to be a useful tool to quantify tumor neovascularization. Specifically, membrane reinforcement could be observed in vessel ECs, indicating the expression of CD31/ PECAM in their surface. The staining of ECs from tumors and from normal tissues was also compared. In this work, the use of MEC13.3 mAb is reported to recognize mice mammary tumor ECs as a useful tool to identify neovascularization. It would also be helpful for research on the origin and function of vascular endothelium in murine tumor experimental models.
author	Gazzaniga, Silvina N. Wainstok de Calmanovici, Rosa
author_facet	Gazzaniga, Silvina N. Wainstok de Calmanovici, Rosa
author_sort	Gazzaniga, Silvina N.
title	Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors
title_short	Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors
title_full	Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors
title_fullStr	Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors
title_full_unstemmed	Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors
title_sort	detection of endothelial cells by mec 13.3 monoclonal antibody in mice mammary tumors
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v21_n1_p39_Vanzulli http://hdl.handle.net/20.500.12110/paper_03279545_v21_n1_p39_Vanzulli
work_keys_str_mv	AT gazzanigasilvinan detectionofendothelialcellsbymec133monoclonalantibodyinmicemammarytumors AT wainstokdecalmanovicirosa detectionofendothelialcellsbymec133monoclonalantibodyinmicemammarytumors
_version_	1768542504982937600

Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors

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