The adenohypophysis of the swamp eel, Synbranchus marmoratus, an immunocytochemical analysis
The adenohypophyseal cell types of the protogynous fish Synbranchus marmoratus were studied by histochemical and immunocytochemical staining with antisera raised against piscine and human pituitary hormones to ascertain their distribution. The prolactin (PRL) cells were distributed in the rostral pa...
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1996
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n2_p155_Vissio http://hdl.handle.net/20.500.12110/paper_03279545_v20_n2_p155_Vissio |
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paper:paper_03279545_v20_n2_p155_Vissio2023-06-08T15:33:36Z The adenohypophysis of the swamp eel, Synbranchus marmoratus, an immunocytochemical analysis Immunocytochemistry Light microscopy Pituitary cells Pituitary gland Synbranchiformes adenohypophysis hormone gonadotropin prolactin adenohypophysis animal article cytology eel female histology human immunohistochemistry immunology male metabolism Animals Eels Female Gonadotropins, Pituitary Humans Immunohistochemistry Male Pituitary Gland, Anterior Pituitary Hormones, Anterior Prolactin The adenohypophyseal cell types of the protogynous fish Synbranchus marmoratus were studied by histochemical and immunocytochemical staining with antisera raised against piscine and human pituitary hormones to ascertain their distribution. The prolactin (PRL) cells were distributed in the rostral pars distalis and showed specific binding to antisera to carp and chum salmon prolactin. No reaction was observed with antiserum to human prolactin. The corticotrops showed strong immunoreactivity with anti-human ACTH, these cells bordered the neurohypophysis and islets between PRL cells in the rostral pars distalis. Growth hormone (GH) cells were densely distributed and associated with the neurohypophysis only in pars distalis proximal. They reacted with antisera to piscine GH but not with antisera to human growth hormone. The thyrotrops were scattered in the proximal pars distalis and showed strong immunoreactivity to the human thyrotropin Beta subunit antiserum. Gonadotrops were located in the central area of the proximal pars distalis and in the external border of the pars intermedia. These cells were alcian blue and PAS positive, and reacted with anti-croaker GTH and anti-coho GTH I and GTH II. The PAS positive cells from the pars intermedia bound specifically to anti-chum somatolactin. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n2_p155_Vissio http://hdl.handle.net/20.500.12110/paper_03279545_v20_n2_p155_Vissio |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Immunocytochemistry Light microscopy Pituitary cells Pituitary gland Synbranchiformes adenohypophysis hormone gonadotropin prolactin adenohypophysis animal article cytology eel female histology human immunohistochemistry immunology male metabolism Animals Eels Female Gonadotropins, Pituitary Humans Immunohistochemistry Male Pituitary Gland, Anterior Pituitary Hormones, Anterior Prolactin |
spellingShingle |
Immunocytochemistry Light microscopy Pituitary cells Pituitary gland Synbranchiformes adenohypophysis hormone gonadotropin prolactin adenohypophysis animal article cytology eel female histology human immunohistochemistry immunology male metabolism Animals Eels Female Gonadotropins, Pituitary Humans Immunohistochemistry Male Pituitary Gland, Anterior Pituitary Hormones, Anterior Prolactin The adenohypophysis of the swamp eel, Synbranchus marmoratus, an immunocytochemical analysis |
topic_facet |
Immunocytochemistry Light microscopy Pituitary cells Pituitary gland Synbranchiformes adenohypophysis hormone gonadotropin prolactin adenohypophysis animal article cytology eel female histology human immunohistochemistry immunology male metabolism Animals Eels Female Gonadotropins, Pituitary Humans Immunohistochemistry Male Pituitary Gland, Anterior Pituitary Hormones, Anterior Prolactin |
description |
The adenohypophyseal cell types of the protogynous fish Synbranchus marmoratus were studied by histochemical and immunocytochemical staining with antisera raised against piscine and human pituitary hormones to ascertain their distribution. The prolactin (PRL) cells were distributed in the rostral pars distalis and showed specific binding to antisera to carp and chum salmon prolactin. No reaction was observed with antiserum to human prolactin. The corticotrops showed strong immunoreactivity with anti-human ACTH, these cells bordered the neurohypophysis and islets between PRL cells in the rostral pars distalis. Growth hormone (GH) cells were densely distributed and associated with the neurohypophysis only in pars distalis proximal. They reacted with antisera to piscine GH but not with antisera to human growth hormone. The thyrotrops were scattered in the proximal pars distalis and showed strong immunoreactivity to the human thyrotropin Beta subunit antiserum. Gonadotrops were located in the central area of the proximal pars distalis and in the external border of the pars intermedia. These cells were alcian blue and PAS positive, and reacted with anti-croaker GTH and anti-coho GTH I and GTH II. The PAS positive cells from the pars intermedia bound specifically to anti-chum somatolactin. |
title |
The adenohypophysis of the swamp eel, Synbranchus marmoratus, an immunocytochemical analysis |
title_short |
The adenohypophysis of the swamp eel, Synbranchus marmoratus, an immunocytochemical analysis |
title_full |
The adenohypophysis of the swamp eel, Synbranchus marmoratus, an immunocytochemical analysis |
title_fullStr |
The adenohypophysis of the swamp eel, Synbranchus marmoratus, an immunocytochemical analysis |
title_full_unstemmed |
The adenohypophysis of the swamp eel, Synbranchus marmoratus, an immunocytochemical analysis |
title_sort |
adenohypophysis of the swamp eel, synbranchus marmoratus, an immunocytochemical analysis |
publishDate |
1996 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03279545_v20_n2_p155_Vissio http://hdl.handle.net/20.500.12110/paper_03279545_v20_n2_p155_Vissio |
_version_ |
1768546630668124160 |