Porphyrinogen carboxylyase. Studies on the existence of isoenzymes.

Porphyrinogen carboxylyase from normal rat liver was subjected to purification methods. Two different purification protocols were used. In both cases, the initial steps consisted in obtaining a liver homogenate, followed by centrifugation, salt precipitation and phosphate gel absorption. Scheme I co...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ríos de Molina, María del Carmen, Chaufan, Rosa Gabriela, Iglesias, Sergio Ricardo, Billi de Catabbi, Silvia Cristina, San Martín de Viale, Leonor Carmen
Publicado:	1996
Materias:	isoenzyme porphyrin porphyrinogen animal article comparative study enzymology isolation and purification liver metabolism rat Animals Isoenzymes Liver Porphyrinogens Porphyrins Rats
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03276309_v46_n4_p265_RiosdeMolina http://hdl.handle.net/20.500.12110/paper_03276309_v46_n4_p265_RiosdeMolina
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03276309_v46_n4_p265_RiosdeMolina
record_format	dspace
spelling	paper:paper_03276309_v46_n4_p265_RiosdeMolina2023-06-08T15:33:27Z Porphyrinogen carboxylyase. Studies on the existence of isoenzymes. Ríos de Molina, María del Carmen Chaufan, Rosa Gabriela Iglesias, Sergio Ricardo Billi de Catabbi, Silvia Cristina San Martín de Viale, Leonor Carmen isoenzyme porphyrin porphyrinogen animal article comparative study enzymology isolation and purification liver metabolism rat Animals Isoenzymes Liver Porphyrinogens Porphyrins Rats Porphyrinogen carboxylyase from normal rat liver was subjected to purification methods. Two different purification protocols were used. In both cases, the initial steps consisted in obtaining a liver homogenate, followed by centrifugation, salt precipitation and phosphate gel absorption. Scheme I consisted in then submitted the preparation to DEAE-cellulose, followed by Sephacryl S-200 and Phenyl-sepharose sequential column chromatographies. Scheme II involved an affinity column followed by a Sephadex G-75 gel filtration column. In both cases, the enzyme was stored at -20 degrees C until its assay. The addition of 2mM dithiotreytol to the incubation media or to the enzyme extract before storage, did not help improve the activity nor the stability of the enzyme. Those fractions containing the maximal enzyme activity, detected using Uroporphyrinogen III or Pentacarboxy-porphyrinogen III as substrate, were not always present in the same tubes for the different columns employed. In addition, the degree of purification obtained in some steps was different according to the substrate employed. The results suggest the existence of at least two isoenzymes for rat liver porphyrinogen carboxy-lyase. Fil:Ríos de Molina, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Chaufan, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iglesias, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Billi de Catabbi, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:San Martín de Viale, L.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03276309_v46_n4_p265_RiosdeMolina http://hdl.handle.net/20.500.12110/paper_03276309_v46_n4_p265_RiosdeMolina
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	isoenzyme porphyrin porphyrinogen animal article comparative study enzymology isolation and purification liver metabolism rat Animals Isoenzymes Liver Porphyrinogens Porphyrins Rats
spellingShingle	isoenzyme porphyrin porphyrinogen animal article comparative study enzymology isolation and purification liver metabolism rat Animals Isoenzymes Liver Porphyrinogens Porphyrins Rats Ríos de Molina, María del Carmen Chaufan, Rosa Gabriela Iglesias, Sergio Ricardo Billi de Catabbi, Silvia Cristina San Martín de Viale, Leonor Carmen Porphyrinogen carboxylyase. Studies on the existence of isoenzymes.
topic_facet	isoenzyme porphyrin porphyrinogen animal article comparative study enzymology isolation and purification liver metabolism rat Animals Isoenzymes Liver Porphyrinogens Porphyrins Rats
description	Porphyrinogen carboxylyase from normal rat liver was subjected to purification methods. Two different purification protocols were used. In both cases, the initial steps consisted in obtaining a liver homogenate, followed by centrifugation, salt precipitation and phosphate gel absorption. Scheme I consisted in then submitted the preparation to DEAE-cellulose, followed by Sephacryl S-200 and Phenyl-sepharose sequential column chromatographies. Scheme II involved an affinity column followed by a Sephadex G-75 gel filtration column. In both cases, the enzyme was stored at -20 degrees C until its assay. The addition of 2mM dithiotreytol to the incubation media or to the enzyme extract before storage, did not help improve the activity nor the stability of the enzyme. Those fractions containing the maximal enzyme activity, detected using Uroporphyrinogen III or Pentacarboxy-porphyrinogen III as substrate, were not always present in the same tubes for the different columns employed. In addition, the degree of purification obtained in some steps was different according to the substrate employed. The results suggest the existence of at least two isoenzymes for rat liver porphyrinogen carboxy-lyase.
author	Ríos de Molina, María del Carmen Chaufan, Rosa Gabriela Iglesias, Sergio Ricardo Billi de Catabbi, Silvia Cristina San Martín de Viale, Leonor Carmen
author_facet	Ríos de Molina, María del Carmen Chaufan, Rosa Gabriela Iglesias, Sergio Ricardo Billi de Catabbi, Silvia Cristina San Martín de Viale, Leonor Carmen
author_sort	Ríos de Molina, María del Carmen
title	Porphyrinogen carboxylyase. Studies on the existence of isoenzymes.
title_short	Porphyrinogen carboxylyase. Studies on the existence of isoenzymes.
title_full	Porphyrinogen carboxylyase. Studies on the existence of isoenzymes.
title_fullStr	Porphyrinogen carboxylyase. Studies on the existence of isoenzymes.
title_full_unstemmed	Porphyrinogen carboxylyase. Studies on the existence of isoenzymes.
title_sort	porphyrinogen carboxylyase. studies on the existence of isoenzymes.
publishDate	1996
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03276309_v46_n4_p265_RiosdeMolina http://hdl.handle.net/20.500.12110/paper_03276309_v46_n4_p265_RiosdeMolina
work_keys_str_mv	AT riosdemolinamariadelcarmen porphyrinogencarboxylyasestudiesontheexistenceofisoenzymes AT chaufanrosagabriela porphyrinogencarboxylyasestudiesontheexistenceofisoenzymes AT iglesiassergioricardo porphyrinogencarboxylyasestudiesontheexistenceofisoenzymes AT billidecatabbisilviacristina porphyrinogencarboxylyasestudiesontheexistenceofisoenzymes AT sanmartindevialeleonorcarmen porphyrinogencarboxylyasestudiesontheexistenceofisoenzymes
_version_	1768544133289345024

Porphyrinogen carboxylyase. Studies on the existence of isoenzymes.

Ejemplares similares