Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole
Metronidazole (MTZ) is a wonderful drug, which is used clinically to treat a wide range of bacterial and protozoal infections. This study aimed to achieve a precise characterization of the cytotoxic and genotoxic activities of MTZ in cultured human lymphocytes at therapeutic concentrations and to ev...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03262383_v29_n8_p1319_LopezNigro http://hdl.handle.net/20.500.12110/paper_03262383_v29_n8_p1319_LopezNigro |
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paper:paper_03262383_v29_n8_p1319_LopezNigro2023-06-08T15:33:01Z Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole Mudry, Marta Dolores Cell death Cytotoxicity Genetic toxicology Genotoxicity Metronidazole Short term tests metronidazole adult apoptosis article cell death cell viability chromosome aberration chromosome replication concentration response controlled study diploidy DNA content DNA structure drug cytotoxicity female genotoxicity human human cell lymphocyte male mitosis inhibition sister chromatid exchange toxicity testing Metronidazole (MTZ) is a wonderful drug, which is used clinically to treat a wide range of bacterial and protozoal infections. This study aimed to achieve a precise characterization of the cytotoxic and genotoxic activities of MTZ in cultured human lymphocytes at therapeutic concentrations and to evaluate the possible cell death mechanism associated with it. A significant decrease in Mitotic Index (P < 0.001) as well as an increase in Sister Chromatid Exchange (P < 0.001) and Chromosomal Aberrations (P < 0.001) frequencies with no modifications in Replication Index was observed. DNA extracts of MTZ treated cells resulted in nucleosomal DNA ladder pattern after 48 h of cell treatment and this pattern correlated with a decrease in cellular viability (P < 0.001), morphological evidence of apoptosis and increase in the percentage of nuclei with hypodiploid DNA content (P < 0.001). We concluded that MTZ is genotoxic, cytotoxic and is able to modulate cell death through apoptotic mechanisms in the experimental design employed. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03262383_v29_n8_p1319_LopezNigro http://hdl.handle.net/20.500.12110/paper_03262383_v29_n8_p1319_LopezNigro |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Cell death Cytotoxicity Genetic toxicology Genotoxicity Metronidazole Short term tests metronidazole adult apoptosis article cell death cell viability chromosome aberration chromosome replication concentration response controlled study diploidy DNA content DNA structure drug cytotoxicity female genotoxicity human human cell lymphocyte male mitosis inhibition sister chromatid exchange toxicity testing |
spellingShingle |
Cell death Cytotoxicity Genetic toxicology Genotoxicity Metronidazole Short term tests metronidazole adult apoptosis article cell death cell viability chromosome aberration chromosome replication concentration response controlled study diploidy DNA content DNA structure drug cytotoxicity female genotoxicity human human cell lymphocyte male mitosis inhibition sister chromatid exchange toxicity testing Mudry, Marta Dolores Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole |
topic_facet |
Cell death Cytotoxicity Genetic toxicology Genotoxicity Metronidazole Short term tests metronidazole adult apoptosis article cell death cell viability chromosome aberration chromosome replication concentration response controlled study diploidy DNA content DNA structure drug cytotoxicity female genotoxicity human human cell lymphocyte male mitosis inhibition sister chromatid exchange toxicity testing |
description |
Metronidazole (MTZ) is a wonderful drug, which is used clinically to treat a wide range of bacterial and protozoal infections. This study aimed to achieve a precise characterization of the cytotoxic and genotoxic activities of MTZ in cultured human lymphocytes at therapeutic concentrations and to evaluate the possible cell death mechanism associated with it. A significant decrease in Mitotic Index (P < 0.001) as well as an increase in Sister Chromatid Exchange (P < 0.001) and Chromosomal Aberrations (P < 0.001) frequencies with no modifications in Replication Index was observed. DNA extracts of MTZ treated cells resulted in nucleosomal DNA ladder pattern after 48 h of cell treatment and this pattern correlated with a decrease in cellular viability (P < 0.001), morphological evidence of apoptosis and increase in the percentage of nuclei with hypodiploid DNA content (P < 0.001). We concluded that MTZ is genotoxic, cytotoxic and is able to modulate cell death through apoptotic mechanisms in the experimental design employed. |
author |
Mudry, Marta Dolores |
author_facet |
Mudry, Marta Dolores |
author_sort |
Mudry, Marta Dolores |
title |
Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole |
title_short |
Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole |
title_full |
Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole |
title_fullStr |
Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole |
title_full_unstemmed |
Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole |
title_sort |
use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of metronidazole |
publishDate |
2010 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03262383_v29_n8_p1319_LopezNigro http://hdl.handle.net/20.500.12110/paper_03262383_v29_n8_p1319_LopezNigro |
work_keys_str_mv |
AT mudrymartadolores useofshorttermgenotoxicologicalteststostudycytotoxicgenotoxicandcelldeathmechanismofmetronidazole |
_version_ |
1768543800914870272 |