In vitro growth and cell wall degrading enzyme production by Argentinean isolates of Macrophomina phaseolina, the causative agent of charcoal rot in corn
Macrophomina phaseolina is a polyphagous phytopathogen, causing stalk rot on many commercially important species. Damages caused by this pathogen in soybean and maize crops in Argentina during drought and hot weather have increased due its ability to survive as sclerotia in soil and crop debris unde...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v48_n4_p267_Ramos http://hdl.handle.net/20.500.12110/paper_03257541_v48_n4_p267_Ramos |
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paper:paper_03257541_v48_n4_p267_Ramos2023-06-08T15:32:57Z In vitro growth and cell wall degrading enzyme production by Argentinean isolates of Macrophomina phaseolina, the causative agent of charcoal rot in corn Ramos, Araceli Marcela Gally, Marcela Edith Carabajal, Maira Lia Levin, Laura Noemi Cell wall-degrading enzymes Charcoal rot Corn Macrophomina phaseolina carboxymethylcellulose cellulase charcoal glucose glutamic acid laccase nitrogen pectin polygalacturonase xylan carbon culture medium endo 1,4 beta xylanase fungal protein glycosidase polymethylgalacturonase Argentina Article cell wall cytolysis disease severity enzymatic degradation enzyme activity enzyme assay enzyme synthesis fungal biomass fungus growth Helianthus annuus in vitro study Macrophomina phaseolina maize nonhuman pathogenicity skin maceration submerged fermentation watermelon Ascomycetes cell wall Citrullus comparative study culture medium enzymology fermentation growth, development and aging isolation and purification metabolism microbiology plant seed procedures sunflower Argentina Ascomycota Carbon Cell Wall Cellulase Citrullus Culture Media Endo-1,4-beta Xylanases Fermentation Fungal Proteins Glycoside Hydrolases Helianthus Industrial Microbiology Laccase Nitrogen Polygalacturonase Seeds Zea mays Macrophomina phaseolina is a polyphagous phytopathogen, causing stalk rot on many commercially important species. Damages caused by this pathogen in soybean and maize crops in Argentina during drought and hot weather have increased due its ability to survive as sclerotia in soil and crop debris under non-till practices. In this work, we explored the in vitro production of plant cell wall-degrading enzymes [pectinases (polygalacturonase and polymethylgalacturonase); cellulases (endoglucanase); hemicellulases (endoxylanase) and the ligninolytic enzyme laccase] by several Argentinean isolates of M. phaseolina, and assessed the pathogenicity of these isolates as a preliminary step to establish the role of these enzymes in M. phaseolina–maize interaction. The isolates were grown in liquid synthetic medium supplemented with glucose, pectin, carboxymethylcellulose or xylan as carbon sources and/or enzyme inducers and glutamic acid as nitrogen source. Pectinases were the first cell wall-degrading enzymes detected and the activities obtained (polygalacturonase activity was between 0.4 and 1.3 U/ml and polymethylgalacturonase between 0.15 and 1.3 U/ml) were higher than those of cellulases and xylanases, which appeared later and in a lesser magnitude. This sequence would promote initial tissue maceration followed by cell wall degradation. Laccase was detected in all the isolates evaluated (activity was between 36 U/l and 63 U/l). The aggressiveness of the isolates was tested in maize, sunflower and watermelon seeds, being high on all the plants assayed. This study reports for the first time the potential of different isolates of M. phaseolina to produce plant cell wall-degrading enzymes in submerged fermentation. © 2016 Asociación Argentina de Microbiología Fil:Ramos, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gally, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carabajal, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v48_n4_p267_Ramos http://hdl.handle.net/20.500.12110/paper_03257541_v48_n4_p267_Ramos |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cell wall-degrading enzymes Charcoal rot Corn Macrophomina phaseolina carboxymethylcellulose cellulase charcoal glucose glutamic acid laccase nitrogen pectin polygalacturonase xylan carbon culture medium endo 1,4 beta xylanase fungal protein glycosidase polymethylgalacturonase Argentina Article cell wall cytolysis disease severity enzymatic degradation enzyme activity enzyme assay enzyme synthesis fungal biomass fungus growth Helianthus annuus in vitro study Macrophomina phaseolina maize nonhuman pathogenicity skin maceration submerged fermentation watermelon Ascomycetes cell wall Citrullus comparative study culture medium enzymology fermentation growth, development and aging isolation and purification metabolism microbiology plant seed procedures sunflower Argentina Ascomycota Carbon Cell Wall Cellulase Citrullus Culture Media Endo-1,4-beta Xylanases Fermentation Fungal Proteins Glycoside Hydrolases Helianthus Industrial Microbiology Laccase Nitrogen Polygalacturonase Seeds Zea mays |
| spellingShingle |
Cell wall-degrading enzymes Charcoal rot Corn Macrophomina phaseolina carboxymethylcellulose cellulase charcoal glucose glutamic acid laccase nitrogen pectin polygalacturonase xylan carbon culture medium endo 1,4 beta xylanase fungal protein glycosidase polymethylgalacturonase Argentina Article cell wall cytolysis disease severity enzymatic degradation enzyme activity enzyme assay enzyme synthesis fungal biomass fungus growth Helianthus annuus in vitro study Macrophomina phaseolina maize nonhuman pathogenicity skin maceration submerged fermentation watermelon Ascomycetes cell wall Citrullus comparative study culture medium enzymology fermentation growth, development and aging isolation and purification metabolism microbiology plant seed procedures sunflower Argentina Ascomycota Carbon Cell Wall Cellulase Citrullus Culture Media Endo-1,4-beta Xylanases Fermentation Fungal Proteins Glycoside Hydrolases Helianthus Industrial Microbiology Laccase Nitrogen Polygalacturonase Seeds Zea mays Ramos, Araceli Marcela Gally, Marcela Edith Carabajal, Maira Lia Levin, Laura Noemi In vitro growth and cell wall degrading enzyme production by Argentinean isolates of Macrophomina phaseolina, the causative agent of charcoal rot in corn |
| topic_facet |
Cell wall-degrading enzymes Charcoal rot Corn Macrophomina phaseolina carboxymethylcellulose cellulase charcoal glucose glutamic acid laccase nitrogen pectin polygalacturonase xylan carbon culture medium endo 1,4 beta xylanase fungal protein glycosidase polymethylgalacturonase Argentina Article cell wall cytolysis disease severity enzymatic degradation enzyme activity enzyme assay enzyme synthesis fungal biomass fungus growth Helianthus annuus in vitro study Macrophomina phaseolina maize nonhuman pathogenicity skin maceration submerged fermentation watermelon Ascomycetes cell wall Citrullus comparative study culture medium enzymology fermentation growth, development and aging isolation and purification metabolism microbiology plant seed procedures sunflower Argentina Ascomycota Carbon Cell Wall Cellulase Citrullus Culture Media Endo-1,4-beta Xylanases Fermentation Fungal Proteins Glycoside Hydrolases Helianthus Industrial Microbiology Laccase Nitrogen Polygalacturonase Seeds Zea mays |
| description |
Macrophomina phaseolina is a polyphagous phytopathogen, causing stalk rot on many commercially important species. Damages caused by this pathogen in soybean and maize crops in Argentina during drought and hot weather have increased due its ability to survive as sclerotia in soil and crop debris under non-till practices. In this work, we explored the in vitro production of plant cell wall-degrading enzymes [pectinases (polygalacturonase and polymethylgalacturonase); cellulases (endoglucanase); hemicellulases (endoxylanase) and the ligninolytic enzyme laccase] by several Argentinean isolates of M. phaseolina, and assessed the pathogenicity of these isolates as a preliminary step to establish the role of these enzymes in M. phaseolina–maize interaction. The isolates were grown in liquid synthetic medium supplemented with glucose, pectin, carboxymethylcellulose or xylan as carbon sources and/or enzyme inducers and glutamic acid as nitrogen source. Pectinases were the first cell wall-degrading enzymes detected and the activities obtained (polygalacturonase activity was between 0.4 and 1.3 U/ml and polymethylgalacturonase between 0.15 and 1.3 U/ml) were higher than those of cellulases and xylanases, which appeared later and in a lesser magnitude. This sequence would promote initial tissue maceration followed by cell wall degradation. Laccase was detected in all the isolates evaluated (activity was between 36 U/l and 63 U/l). The aggressiveness of the isolates was tested in maize, sunflower and watermelon seeds, being high on all the plants assayed. This study reports for the first time the potential of different isolates of M. phaseolina to produce plant cell wall-degrading enzymes in submerged fermentation. © 2016 Asociación Argentina de Microbiología |
| author |
Ramos, Araceli Marcela Gally, Marcela Edith Carabajal, Maira Lia Levin, Laura Noemi |
| author_facet |
Ramos, Araceli Marcela Gally, Marcela Edith Carabajal, Maira Lia Levin, Laura Noemi |
| author_sort |
Ramos, Araceli Marcela |
| title |
In vitro growth and cell wall degrading enzyme production by Argentinean isolates of Macrophomina phaseolina, the causative agent of charcoal rot in corn |
| title_short |
In vitro growth and cell wall degrading enzyme production by Argentinean isolates of Macrophomina phaseolina, the causative agent of charcoal rot in corn |
| title_full |
In vitro growth and cell wall degrading enzyme production by Argentinean isolates of Macrophomina phaseolina, the causative agent of charcoal rot in corn |
| title_fullStr |
In vitro growth and cell wall degrading enzyme production by Argentinean isolates of Macrophomina phaseolina, the causative agent of charcoal rot in corn |
| title_full_unstemmed |
In vitro growth and cell wall degrading enzyme production by Argentinean isolates of Macrophomina phaseolina, the causative agent of charcoal rot in corn |
| title_sort |
in vitro growth and cell wall degrading enzyme production by argentinean isolates of macrophomina phaseolina, the causative agent of charcoal rot in corn |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v48_n4_p267_Ramos http://hdl.handle.net/20.500.12110/paper_03257541_v48_n4_p267_Ramos |
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