Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity

This study was conducted to compare different liquid culture media for Paenibacillus larvae growth in order to find the best one to be used in studies on activity of antimicrobial substances, such as essential oils. P. larvae presented poor growth in usual broths such as Mueller-Hinton, commonly emp...

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Publicado: 2008
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v40_n3_p147_Gende
http://hdl.handle.net/20.500.12110/paper_03257541_v40_n3_p147_Gende
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spelling paper:paper_03257541_v40_n3_p147_Gende2023-06-08T15:32:53Z Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity Antimicrobial activity Culture media Paenibacillus larvae antimicrobial activity article bacterial growth bacterial strain bacterium culture controlled study culture medium nonhuman Paenibacillus Culture Media Gram-Positive Endospore-Forming Bacteria Microbial Sensitivity Tests This study was conducted to compare different liquid culture media for Paenibacillus larvae growth in order to find the best one to be used in studies on activity of antimicrobial substances, such as essential oils. P. larvae presented poor growth in usual broths such as Mueller-Hinton, commonly employed in antimicrobial activity assays. Growth in liquid media was evaluated using Paenibacillus larvae strains isolated from hives located in different geographical zones. The MYT medium (Mueller-Hinton broth, yeast extract and thiamine) was selected out of the eight liquid media analyzed, as it proved to be the most adequate due to its higher absorbance at 620 nm. The following mean values were obtained from the four P. larvae strains: 0.227 ± 0.016 for the Cobo strain, 0.279 ± 0.015 for La Plata strain, 0.758 ± 0.020 for Mechongué strain and 0.244 ± 0.0079 for Sierra de los Padres strain, respectively. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v40_n3_p147_Gende http://hdl.handle.net/20.500.12110/paper_03257541_v40_n3_p147_Gende
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Antimicrobial activity
Culture media
Paenibacillus larvae
antimicrobial activity
article
bacterial growth
bacterial strain
bacterium culture
controlled study
culture medium
nonhuman
Paenibacillus
Culture Media
Gram-Positive Endospore-Forming Bacteria
Microbial Sensitivity Tests
spellingShingle Antimicrobial activity
Culture media
Paenibacillus larvae
antimicrobial activity
article
bacterial growth
bacterial strain
bacterium culture
controlled study
culture medium
nonhuman
Paenibacillus
Culture Media
Gram-Positive Endospore-Forming Bacteria
Microbial Sensitivity Tests
Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity
topic_facet Antimicrobial activity
Culture media
Paenibacillus larvae
antimicrobial activity
article
bacterial growth
bacterial strain
bacterium culture
controlled study
culture medium
nonhuman
Paenibacillus
Culture Media
Gram-Positive Endospore-Forming Bacteria
Microbial Sensitivity Tests
description This study was conducted to compare different liquid culture media for Paenibacillus larvae growth in order to find the best one to be used in studies on activity of antimicrobial substances, such as essential oils. P. larvae presented poor growth in usual broths such as Mueller-Hinton, commonly employed in antimicrobial activity assays. Growth in liquid media was evaluated using Paenibacillus larvae strains isolated from hives located in different geographical zones. The MYT medium (Mueller-Hinton broth, yeast extract and thiamine) was selected out of the eight liquid media analyzed, as it proved to be the most adequate due to its higher absorbance at 620 nm. The following mean values were obtained from the four P. larvae strains: 0.227 ± 0.016 for the Cobo strain, 0.279 ± 0.015 for La Plata strain, 0.758 ± 0.020 for Mechongué strain and 0.244 ± 0.0079 for Sierra de los Padres strain, respectively.
title Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity
title_short Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity
title_full Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity
title_fullStr Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity
title_full_unstemmed Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity
title_sort evaluation of culture media for paenibacillus larvae applied to studies of antimicrobial activity
publishDate 2008
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v40_n3_p147_Gende
http://hdl.handle.net/20.500.12110/paper_03257541_v40_n3_p147_Gende
_version_ 1768544041147826176