Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis

The influence of temperature and pH on the activity and stability of the cellulase system (endoglucanase, exoglucanase and cellobiase) was investigated in Nectria catalinensis. Optimal temperature for the activity of the cellulase system ranged from 50 to 55 °C, with an optimum for stability between...

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Autores principales:	Pardo, Alejandro Guillermo, Forchíassin, Flavia
Publicado:	1999
Materias:	Cellulases Fungi Nechia catalinensis beta glucosidase cellulase exoglucanase glucan synthase glucose unclassified drug article ascomycetes enzyme activity enzyme kinetics enzyme stability nectria catalinensis nonhuman pH measurement temperature sensitivity Cellulase Enzyme Stability Hydrogen-Ion Concentration Hypocreales Temperature
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v31_n1_p31_Pardo http://hdl.handle.net/20.500.12110/paper_03257541_v31_n1_p31_Pardo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03257541_v31_n1_p31_Pardo
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spelling	paper:paper_03257541_v31_n1_p31_Pardo2023-06-08T15:32:45Z Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis Pardo, Alejandro Guillermo Forchíassin, Flavia Cellulases Fungi Nechia catalinensis beta glucosidase cellulase exoglucanase glucan synthase glucose unclassified drug article ascomycetes enzyme activity enzyme kinetics enzyme stability nectria catalinensis nonhuman pH measurement temperature sensitivity Cellulase Enzyme Stability Hydrogen-Ion Concentration Hypocreales Temperature The influence of temperature and pH on the activity and stability of the cellulase system (endoglucanase, exoglucanase and cellobiase) was investigated in Nectria catalinensis. Optimal temperature for the activity of the cellulase system ranged from 50 to 55 °C, with an optimum for stability between 23 °C and 37 °C after a 72 h incubation period. For the different enzymes, maximal activity was registered between pH 4.2-5,8, with pH 4.8 being close to optimal for all stability studies. The activation energy was 4.97 Kcal mol-1 for endoglucanase, 4.37 Kcal mol-1 for exoglucanase and 13.73 Kcal mol-1 for cellobiase. The K(m) and V(max) values were 1.73 mg CMC ml-1 and 0.45 μmol glucose min-1 mg protein-1 for endoglucanase, 0.22 mg microcrystalline cellulose ml-1 and 57.1 nmol glucose min-1 mg protein-1 for exoglucanase, and 2.95 mM cellobiose and 0.17 μmol glucose min-1 mg protein-1 for cellobiase. Fil:Pardo, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1999 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v31_n1_p31_Pardo http://hdl.handle.net/20.500.12110/paper_03257541_v31_n1_p31_Pardo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cellulases Fungi Nechia catalinensis beta glucosidase cellulase exoglucanase glucan synthase glucose unclassified drug article ascomycetes enzyme activity enzyme kinetics enzyme stability nectria catalinensis nonhuman pH measurement temperature sensitivity Cellulase Enzyme Stability Hydrogen-Ion Concentration Hypocreales Temperature
spellingShingle	Cellulases Fungi Nechia catalinensis beta glucosidase cellulase exoglucanase glucan synthase glucose unclassified drug article ascomycetes enzyme activity enzyme kinetics enzyme stability nectria catalinensis nonhuman pH measurement temperature sensitivity Cellulase Enzyme Stability Hydrogen-Ion Concentration Hypocreales Temperature Pardo, Alejandro Guillermo Forchíassin, Flavia Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis
topic_facet	Cellulases Fungi Nechia catalinensis beta glucosidase cellulase exoglucanase glucan synthase glucose unclassified drug article ascomycetes enzyme activity enzyme kinetics enzyme stability nectria catalinensis nonhuman pH measurement temperature sensitivity Cellulase Enzyme Stability Hydrogen-Ion Concentration Hypocreales Temperature
description	The influence of temperature and pH on the activity and stability of the cellulase system (endoglucanase, exoglucanase and cellobiase) was investigated in Nectria catalinensis. Optimal temperature for the activity of the cellulase system ranged from 50 to 55 °C, with an optimum for stability between 23 °C and 37 °C after a 72 h incubation period. For the different enzymes, maximal activity was registered between pH 4.2-5,8, with pH 4.8 being close to optimal for all stability studies. The activation energy was 4.97 Kcal mol-1 for endoglucanase, 4.37 Kcal mol-1 for exoglucanase and 13.73 Kcal mol-1 for cellobiase. The K(m) and V(max) values were 1.73 mg CMC ml-1 and 0.45 μmol glucose min-1 mg protein-1 for endoglucanase, 0.22 mg microcrystalline cellulose ml-1 and 57.1 nmol glucose min-1 mg protein-1 for exoglucanase, and 2.95 mM cellobiose and 0.17 μmol glucose min-1 mg protein-1 for cellobiase.
author	Pardo, Alejandro Guillermo Forchíassin, Flavia
author_facet	Pardo, Alejandro Guillermo Forchíassin, Flavia
author_sort	Pardo, Alejandro Guillermo
title	Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis
title_short	Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis
title_full	Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis
title_fullStr	Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis
title_full_unstemmed	Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis
title_sort	influence of temperature and ph on cellulase activity and stability in nectria catalinensis
publishDate	1999
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v31_n1_p31_Pardo http://hdl.handle.net/20.500.12110/paper_03257541_v31_n1_p31_Pardo
work_keys_str_mv	AT pardoalejandroguillermo influenceoftemperatureandphoncellulaseactivityandstabilityinnectriacatalinensis AT forchiassinflavia influenceoftemperatureandphoncellulaseactivityandstabilityinnectriacatalinensis
_version_	1768544913397383168

Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis

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