Comparative analysis of isoenzyme patterns of beta-1,4 endoglucanase in species of Saccobolus genus (Ascobolaceae-Pezizales)

Growth and beta-1, 4 endoglucanase production of six species of Saccobolus were determined. Twenty nine strains of thirteen geographical isolates were tested, using culture medium with crystalline cellulose as carbon source. All species showed good growth and enzyme activity. A high intraspecific ho...

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Detalles Bibliográficos
Autores principales: Ramos, Araceli Marcela, Forchíassin, Flavia
Publicado: 1998
Materias:
cellulase
isoenzyme
article
Ascomycetes
chemistry
enzymology
isoelectric focusing
Ascomycota
Cellulase
Isoelectric Focusing
Isoenzymes
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v30_n3_p130_Ramos
http://hdl.handle.net/20.500.12110/paper_03257541_v30_n3_p130_Ramos
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03257541_v30_n3_p130_Ramos
record_format dspace
spelling paper:paper_03257541_v30_n3_p130_Ramos2023-06-08T15:32:44Z Comparative analysis of isoenzyme patterns of beta-1,4 endoglucanase in species of Saccobolus genus (Ascobolaceae-Pezizales) Ramos, Araceli Marcela Forchíassin, Flavia cellulase isoenzyme article Ascomycetes chemistry enzymology isoelectric focusing Ascomycota Cellulase Isoelectric Focusing Isoenzymes Growth and beta-1, 4 endoglucanase production of six species of Saccobolus were determined. Twenty nine strains of thirteen geographical isolates were tested, using culture medium with crystalline cellulose as carbon source. All species showed good growth and enzyme activity. A high intraspecific homogeneity was observed as there were no differences in the variables measured within the strains of each geographical isolate and the only difference was between the two isolates of S. infestans. Each species had a characteristic electromorph, showing a variable number of isozymes with a maximum of 11 for S. seudodepauperatus and a minimum of 7 for S. platensis. These results support the usage of isoenzymatic patterns of beta-1, 4 endoglucanase and physiological parameters for the identification of species of Saccobolus as an aid to traditional taxonomy. This methodology is an additional tool to traditional taxonomy, based only on morphological characters. Fil:Ramos, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v30_n3_p130_Ramos http://hdl.handle.net/20.500.12110/paper_03257541_v30_n3_p130_Ramos
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic cellulase
isoenzyme
article
Ascomycetes
chemistry
enzymology
isoelectric focusing
Ascomycota
Cellulase
Isoelectric Focusing
Isoenzymes
spellingShingle cellulase
isoenzyme
article
Ascomycetes
chemistry
enzymology
isoelectric focusing
Ascomycota
Cellulase
Isoelectric Focusing
Isoenzymes
Ramos, Araceli Marcela
Forchíassin, Flavia
Comparative analysis of isoenzyme patterns of beta-1,4 endoglucanase in species of Saccobolus genus (Ascobolaceae-Pezizales)
topic_facet cellulase
isoenzyme
article
Ascomycetes
chemistry
enzymology
isoelectric focusing
Ascomycota
Cellulase
Isoelectric Focusing
Isoenzymes
description Growth and beta-1, 4 endoglucanase production of six species of Saccobolus were determined. Twenty nine strains of thirteen geographical isolates were tested, using culture medium with crystalline cellulose as carbon source. All species showed good growth and enzyme activity. A high intraspecific homogeneity was observed as there were no differences in the variables measured within the strains of each geographical isolate and the only difference was between the two isolates of S. infestans. Each species had a characteristic electromorph, showing a variable number of isozymes with a maximum of 11 for S. seudodepauperatus and a minimum of 7 for S. platensis. These results support the usage of isoenzymatic patterns of beta-1, 4 endoglucanase and physiological parameters for the identification of species of Saccobolus as an aid to traditional taxonomy. This methodology is an additional tool to traditional taxonomy, based only on morphological characters.
author Ramos, Araceli Marcela
Forchíassin, Flavia
author_facet Ramos, Araceli Marcela
Forchíassin, Flavia
author_sort Ramos, Araceli Marcela
title Comparative analysis of isoenzyme patterns of beta-1,4 endoglucanase in species of Saccobolus genus (Ascobolaceae-Pezizales)
title_short Comparative analysis of isoenzyme patterns of beta-1,4 endoglucanase in species of Saccobolus genus (Ascobolaceae-Pezizales)
title_full Comparative analysis of isoenzyme patterns of beta-1,4 endoglucanase in species of Saccobolus genus (Ascobolaceae-Pezizales)
title_fullStr Comparative analysis of isoenzyme patterns of beta-1,4 endoglucanase in species of Saccobolus genus (Ascobolaceae-Pezizales)
title_full_unstemmed Comparative analysis of isoenzyme patterns of beta-1,4 endoglucanase in species of Saccobolus genus (Ascobolaceae-Pezizales)
title_sort comparative analysis of isoenzyme patterns of beta-1,4 endoglucanase in species of saccobolus genus (ascobolaceae-pezizales)
publishDate 1998
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v30_n3_p130_Ramos
http://hdl.handle.net/20.500.12110/paper_03257541_v30_n3_p130_Ramos
work_keys_str_mv AT ramosaracelimarcela comparativeanalysisofisoenzymepatternsofbeta14endoglucanaseinspeciesofsaccobolusgenusascobolaceaepezizales
AT forchiassinflavia comparativeanalysisofisoenzymepatternsofbeta14endoglucanaseinspeciesofsaccobolusgenusascobolaceaepezizales
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