Effect of culture conditions on the production of cellulases by Trametes trogii

Production of the components of the cellulase complex by Trametes trogii was investigated under different culture conditions. Endoglucanase, exoglucanase and beta-glucosidase were produced by Trametes trogii under shaken and stationary conditions using crystalline cellulose as the only carbon source...

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Autores principales:	Levin, Laura Noemi, Forchíassin, Flavia
Publicado:	1997
Materias:	beta glucosidase cellulase fungal protein glucan 1,3 beta glucosidase glucose article Basidiomycetes biosynthesis comparative study culture medium drug effect enzyme induction enzymology gene expression regulation genetics growth, development and aging methodology mycology pH temperature Basidiomycota beta-Glucosidase Cellulase Culture Media Enzyme Induction Fungal Proteins Gene Expression Regulation, Fungal Glucan 1,3-beta-Glucosidase Glucose Hydrogen-Ion Concentration Mycology Temperature
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v29_n1_p16_Levin http://hdl.handle.net/20.500.12110/paper_03257541_v29_n1_p16_Levin
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_03257541_v29_n1_p16_Levin
record_format	dspace
spelling	paper:paper_03257541_v29_n1_p16_Levin2023-06-08T15:32:42Z Effect of culture conditions on the production of cellulases by Trametes trogii Levin, Laura Noemi Forchíassin, Flavia beta glucosidase cellulase fungal protein glucan 1,3 beta glucosidase glucose article Basidiomycetes biosynthesis comparative study culture medium drug effect enzyme induction enzymology gene expression regulation genetics growth, development and aging methodology mycology pH temperature Basidiomycota beta-Glucosidase Cellulase Culture Media Enzyme Induction Fungal Proteins Gene Expression Regulation, Fungal Glucan 1,3-beta-Glucosidase Glucose Hydrogen-Ion Concentration Mycology Temperature Production of the components of the cellulase complex by Trametes trogii was investigated under different culture conditions. Endoglucanase, exoglucanase and beta-glucosidase were produced by Trametes trogii under shaken and stationary conditions using crystalline cellulose as the only carbon source. No marked differences were found for endo- and exoglucanase production between both culture conditions. In shaken cultures beta-glucosidase values doubled those obtained in stationary conditions. All maxima were reached approximately 10 days earlier in static cultures than in shaken ones. The addition of glucose to the culture medium improved growth but decreased abruptly the enzyme production. The optimal pH of the medium for the synthesis of the enzymes was 5.6, and the best incubation temperature was 25-28 degrees C. Enzyme activities were maximal at pH 3.1 and 45 degrees C (endoglucanase), pH 4.1 and 50 degrees C (exoglucanase) and pH 5.3 and 60 degrees C (beta-glucosidase). Fil:Levin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v29_n1_p16_Levin http://hdl.handle.net/20.500.12110/paper_03257541_v29_n1_p16_Levin
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	beta glucosidase cellulase fungal protein glucan 1,3 beta glucosidase glucose article Basidiomycetes biosynthesis comparative study culture medium drug effect enzyme induction enzymology gene expression regulation genetics growth, development and aging methodology mycology pH temperature Basidiomycota beta-Glucosidase Cellulase Culture Media Enzyme Induction Fungal Proteins Gene Expression Regulation, Fungal Glucan 1,3-beta-Glucosidase Glucose Hydrogen-Ion Concentration Mycology Temperature
spellingShingle	beta glucosidase cellulase fungal protein glucan 1,3 beta glucosidase glucose article Basidiomycetes biosynthesis comparative study culture medium drug effect enzyme induction enzymology gene expression regulation genetics growth, development and aging methodology mycology pH temperature Basidiomycota beta-Glucosidase Cellulase Culture Media Enzyme Induction Fungal Proteins Gene Expression Regulation, Fungal Glucan 1,3-beta-Glucosidase Glucose Hydrogen-Ion Concentration Mycology Temperature Levin, Laura Noemi Forchíassin, Flavia Effect of culture conditions on the production of cellulases by Trametes trogii
topic_facet	beta glucosidase cellulase fungal protein glucan 1,3 beta glucosidase glucose article Basidiomycetes biosynthesis comparative study culture medium drug effect enzyme induction enzymology gene expression regulation genetics growth, development and aging methodology mycology pH temperature Basidiomycota beta-Glucosidase Cellulase Culture Media Enzyme Induction Fungal Proteins Gene Expression Regulation, Fungal Glucan 1,3-beta-Glucosidase Glucose Hydrogen-Ion Concentration Mycology Temperature
description	Production of the components of the cellulase complex by Trametes trogii was investigated under different culture conditions. Endoglucanase, exoglucanase and beta-glucosidase were produced by Trametes trogii under shaken and stationary conditions using crystalline cellulose as the only carbon source. No marked differences were found for endo- and exoglucanase production between both culture conditions. In shaken cultures beta-glucosidase values doubled those obtained in stationary conditions. All maxima were reached approximately 10 days earlier in static cultures than in shaken ones. The addition of glucose to the culture medium improved growth but decreased abruptly the enzyme production. The optimal pH of the medium for the synthesis of the enzymes was 5.6, and the best incubation temperature was 25-28 degrees C. Enzyme activities were maximal at pH 3.1 and 45 degrees C (endoglucanase), pH 4.1 and 50 degrees C (exoglucanase) and pH 5.3 and 60 degrees C (beta-glucosidase).
author	Levin, Laura Noemi Forchíassin, Flavia
author_facet	Levin, Laura Noemi Forchíassin, Flavia
author_sort	Levin, Laura Noemi
title	Effect of culture conditions on the production of cellulases by Trametes trogii
title_short	Effect of culture conditions on the production of cellulases by Trametes trogii
title_full	Effect of culture conditions on the production of cellulases by Trametes trogii
title_fullStr	Effect of culture conditions on the production of cellulases by Trametes trogii
title_full_unstemmed	Effect of culture conditions on the production of cellulases by Trametes trogii
title_sort	effect of culture conditions on the production of cellulases by trametes trogii
publishDate	1997
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v29_n1_p16_Levin http://hdl.handle.net/20.500.12110/paper_03257541_v29_n1_p16_Levin
work_keys_str_mv	AT levinlauranoemi effectofcultureconditionsontheproductionofcellulasesbytrametestrogii AT forchiassinflavia effectofcultureconditionsontheproductionofcellulasesbytrametestrogii
_version_	1768542887818035200

Effect of culture conditions on the production of cellulases by Trametes trogii

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