Endoglucanase production in 4 species of the genus Saccobolus
Endoglucanase production was measured in culture filtrates of four species of Saccobolus growing in media containing glucose or crystalline cellulose as the only carbon sources. Enzyme activity was four to seven times higher in the presence of cellulose than glucose. S. saccoboloides showed maximal...
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1996
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v28_n2_p55_Ramos http://hdl.handle.net/20.500.12110/paper_03257541_v28_n2_p55_Ramos |
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paper:paper_03257541_v28_n2_p55_Ramos2023-06-08T15:32:40Z Endoglucanase production in 4 species of the genus Saccobolus Ramos, Araceli Marcela Forchíassin, Flavia cellulase cellulose fungal protein glucose isoenzyme article Ascomycetes biosynthesis classification comparative study drug effect enzyme induction enzymology gene expression regulation genetics metabolism polyacrylamide gel electrophoresis species difference Ascomycota Cellulase Cellulose Electrophoresis, Polyacrylamide Gel Enzyme Induction Fungal Proteins Gene Expression Regulation, Fungal Glucose Isoenzymes Species Specificity Endoglucanase production was measured in culture filtrates of four species of Saccobolus growing in media containing glucose or crystalline cellulose as the only carbon sources. Enzyme activity was four to seven times higher in the presence of cellulose than glucose. S. saccoboloides showed maximal growth and enzyme production. The extracellular proteins secreted during growth on cellulose were separated by polyacrylamide gel electrophoresis and stained for proteins. A zymogram technique was used to visualize bands with endoglucanase activity. The four species showed different protein and isoenzyme patterns. Fil:Ramos, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v28_n2_p55_Ramos http://hdl.handle.net/20.500.12110/paper_03257541_v28_n2_p55_Ramos |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cellulase cellulose fungal protein glucose isoenzyme article Ascomycetes biosynthesis classification comparative study drug effect enzyme induction enzymology gene expression regulation genetics metabolism polyacrylamide gel electrophoresis species difference Ascomycota Cellulase Cellulose Electrophoresis, Polyacrylamide Gel Enzyme Induction Fungal Proteins Gene Expression Regulation, Fungal Glucose Isoenzymes Species Specificity |
| spellingShingle |
cellulase cellulose fungal protein glucose isoenzyme article Ascomycetes biosynthesis classification comparative study drug effect enzyme induction enzymology gene expression regulation genetics metabolism polyacrylamide gel electrophoresis species difference Ascomycota Cellulase Cellulose Electrophoresis, Polyacrylamide Gel Enzyme Induction Fungal Proteins Gene Expression Regulation, Fungal Glucose Isoenzymes Species Specificity Ramos, Araceli Marcela Forchíassin, Flavia Endoglucanase production in 4 species of the genus Saccobolus |
| topic_facet |
cellulase cellulose fungal protein glucose isoenzyme article Ascomycetes biosynthesis classification comparative study drug effect enzyme induction enzymology gene expression regulation genetics metabolism polyacrylamide gel electrophoresis species difference Ascomycota Cellulase Cellulose Electrophoresis, Polyacrylamide Gel Enzyme Induction Fungal Proteins Gene Expression Regulation, Fungal Glucose Isoenzymes Species Specificity |
| description |
Endoglucanase production was measured in culture filtrates of four species of Saccobolus growing in media containing glucose or crystalline cellulose as the only carbon sources. Enzyme activity was four to seven times higher in the presence of cellulose than glucose. S. saccoboloides showed maximal growth and enzyme production. The extracellular proteins secreted during growth on cellulose were separated by polyacrylamide gel electrophoresis and stained for proteins. A zymogram technique was used to visualize bands with endoglucanase activity. The four species showed different protein and isoenzyme patterns. |
| author |
Ramos, Araceli Marcela Forchíassin, Flavia |
| author_facet |
Ramos, Araceli Marcela Forchíassin, Flavia |
| author_sort |
Ramos, Araceli Marcela |
| title |
Endoglucanase production in 4 species of the genus Saccobolus |
| title_short |
Endoglucanase production in 4 species of the genus Saccobolus |
| title_full |
Endoglucanase production in 4 species of the genus Saccobolus |
| title_fullStr |
Endoglucanase production in 4 species of the genus Saccobolus |
| title_full_unstemmed |
Endoglucanase production in 4 species of the genus Saccobolus |
| title_sort |
endoglucanase production in 4 species of the genus saccobolus |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v28_n2_p55_Ramos http://hdl.handle.net/20.500.12110/paper_03257541_v28_n2_p55_Ramos |
| work_keys_str_mv |
AT ramosaracelimarcela endoglucanaseproductionin4speciesofthegenussaccobolus AT forchiassinflavia endoglucanaseproductionin4speciesofthegenussaccobolus |
| _version_ |
1768543898663124992 |