Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis

Fusarium oxysporum f.sp. melonis, a pathogen of melon (Cucumis melo L.), was grown in shaken cultures at 26 degrees C in a mineral salts medium containing glucose, xylan and apple pectin as carbon sources. The extracellular enzymic complex obtained from these cultures showed lytic activity on plant...

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Detalles Bibliográficos
Autor principal: Alconada Magliano, Teresa María
Publicado: 1995
Materias:
cellulase
fungal protein
galacturonic acid
hexuronic acid
polygalacturonase
xylan 1,4 beta xylosidase
xylan endo 1,3 beta xylosidase
article
carrot
cell wall
comparative study
cytology
drug effect
enzymology
extracellular space
fruit
Fusarium
hydrolysis
potato
protoplast
Cell Wall
Cellulase
Daucus carota
Extracellular Space
Fruit
Fungal Proteins
Hexuronic Acids
Hydrolysis
Polygalacturonase
Protoplasts
Solanum tuberosum
Xylan Endo-1,3-beta-Xylosidase
Xylosidases
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v27_n4_p191_Alconada
http://hdl.handle.net/20.500.12110/paper_03257541_v27_n4_p191_Alconada
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Fusarium oxysporum f.sp. melonis, a pathogen of melon (Cucumis melo L.), was grown in shaken cultures at 26 degrees C in a mineral salts medium containing glucose, xylan and apple pectin as carbon sources. The extracellular enzymic complex obtained from these cultures showed lytic activity on plant tissues, causing maceration of melon fruits, potato tubers and carrot roots. Protoplasts were isolated from melon fruits when the maceration was carried out under appropriate osmotic conditions. This fact suggest a possible relationship between the enzymes produced by Fusarium oxysporum f.sp. melonis and their pathogenicity on melon plants.

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