Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L.
Crude extracts from fresh green leaves of Melia azedarach L contain an antiviral factor (FAV) able to inhibit the replication of several animal viruses, e.g. Polio, VSV, HSV, FMDV, Sindbis, Junín, Pichinde and Tacaribe in Vero or BHK-21 cells. Crude preparations were subjected to different steps of...
Guardado en:
Autores principales: | , |
---|---|
Publicado: |
1985
|
Materias: | |
Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v17_n4_p187_Andrei http://hdl.handle.net/20.500.12110/paper_03257541_v17_n4_p187_Andrei |
Aporte de: |
id |
paper:paper_03257541_v17_n4_p187_Andrei |
---|---|
record_format |
dspace |
spelling |
paper:paper_03257541_v17_n4_p187_Andrei2023-06-08T15:32:32Z Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L. Andrei, Graciela Mónica Coto, Celia Esther antivirus agent plant extract Arenavirus Argentina article cell division cell survival drug effect Herpes virus medicinal plant physiology Poliomyelitis virus Sindbis virus tree virus replication Antiviral Agents Arenaviruses, New World Argentina Cell Division Cell Survival Herpesviridae Plant Extracts Plants, Medicinal Poliovirus Sindbis Virus Trees Virus Replication Crude extracts from fresh green leaves of Melia azedarach L contain an antiviral factor (FAV) able to inhibit the replication of several animal viruses, e.g. Polio, VSV, HSV, FMDV, Sindbis, Junín, Pichinde and Tacaribe in Vero or BHK-21 cells. Crude preparations were subjected to different steps of purification like chromatography on Sephadex G-100 and DEAE-Sephadex. The antiviral activity of G-100 and DEAE fractions was fully conserved, whereas contaminating proteins were lost. Two types of cytotoxicity tests were performed with the different fractions. Two-fold serial dilutions of each of them were added to preformed monolayers of Vero or BHK-21 cells and cellular viability was tested. While crude extracts were toxic at low dilutions (less than or equal to 1:10), G-100 and DEAE fractions were not. The other cytotoxicity assay consisted in seeding the cells in the presence of different concentrations of each fraction. G-100 fraction affected cell growth at low dilutions (less than or equal to 1:5), while DEAE fraction did not. It should be remarked that the purification procedure rendered a partial purified DEAE fraction with an increased specific activity (antiviral activity/mg of protein). It is concluded that an antiviral factor devoid of toxicity exists in M. azedarach L extracts, which exhibited a broad spectrum of antiviral activity. Fil:Andrei, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1985 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v17_n4_p187_Andrei http://hdl.handle.net/20.500.12110/paper_03257541_v17_n4_p187_Andrei |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
antivirus agent plant extract Arenavirus Argentina article cell division cell survival drug effect Herpes virus medicinal plant physiology Poliomyelitis virus Sindbis virus tree virus replication Antiviral Agents Arenaviruses, New World Argentina Cell Division Cell Survival Herpesviridae Plant Extracts Plants, Medicinal Poliovirus Sindbis Virus Trees Virus Replication |
spellingShingle |
antivirus agent plant extract Arenavirus Argentina article cell division cell survival drug effect Herpes virus medicinal plant physiology Poliomyelitis virus Sindbis virus tree virus replication Antiviral Agents Arenaviruses, New World Argentina Cell Division Cell Survival Herpesviridae Plant Extracts Plants, Medicinal Poliovirus Sindbis Virus Trees Virus Replication Andrei, Graciela Mónica Coto, Celia Esther Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L. |
topic_facet |
antivirus agent plant extract Arenavirus Argentina article cell division cell survival drug effect Herpes virus medicinal plant physiology Poliomyelitis virus Sindbis virus tree virus replication Antiviral Agents Arenaviruses, New World Argentina Cell Division Cell Survival Herpesviridae Plant Extracts Plants, Medicinal Poliovirus Sindbis Virus Trees Virus Replication |
description |
Crude extracts from fresh green leaves of Melia azedarach L contain an antiviral factor (FAV) able to inhibit the replication of several animal viruses, e.g. Polio, VSV, HSV, FMDV, Sindbis, Junín, Pichinde and Tacaribe in Vero or BHK-21 cells. Crude preparations were subjected to different steps of purification like chromatography on Sephadex G-100 and DEAE-Sephadex. The antiviral activity of G-100 and DEAE fractions was fully conserved, whereas contaminating proteins were lost. Two types of cytotoxicity tests were performed with the different fractions. Two-fold serial dilutions of each of them were added to preformed monolayers of Vero or BHK-21 cells and cellular viability was tested. While crude extracts were toxic at low dilutions (less than or equal to 1:10), G-100 and DEAE fractions were not. The other cytotoxicity assay consisted in seeding the cells in the presence of different concentrations of each fraction. G-100 fraction affected cell growth at low dilutions (less than or equal to 1:5), while DEAE fraction did not. It should be remarked that the purification procedure rendered a partial purified DEAE fraction with an increased specific activity (antiviral activity/mg of protein). It is concluded that an antiviral factor devoid of toxicity exists in M. azedarach L extracts, which exhibited a broad spectrum of antiviral activity. |
author |
Andrei, Graciela Mónica Coto, Celia Esther |
author_facet |
Andrei, Graciela Mónica Coto, Celia Esther |
author_sort |
Andrei, Graciela Mónica |
title |
Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L. |
title_short |
Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L. |
title_full |
Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L. |
title_fullStr |
Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L. |
title_full_unstemmed |
Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L. |
title_sort |
assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of melia azedarach l. |
publishDate |
1985 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v17_n4_p187_Andrei http://hdl.handle.net/20.500.12110/paper_03257541_v17_n4_p187_Andrei |
work_keys_str_mv |
AT andreigracielamonica assaysofcytotoxicityandantiviralactivityofcrudeandsemipurifiedextractsofgreenleavesofmeliaazedarachl AT cotoceliaesther assaysofcytotoxicityandantiviralactivityofcrudeandsemipurifiedextractsofgreenleavesofmeliaazedarachl |
_version_ |
1768541939093733376 |