Cotinine detection and tobacco smoke exposure
Tobacco is one of the major preventable causes of morbidity and premature mortality in the world. A simple, rapid method for determination of cotinine, major metabolite of nicotine in urine, is described. The assay involves a fast liquid-liquid extraction with chloroform as a solvent in alkaline env...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v40_n2_p181_Vacchino http://hdl.handle.net/20.500.12110/paper_03252957_v40_n2_p181_Vacchino |
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paper:paper_03252957_v40_n2_p181_Vacchino2023-06-08T15:32:20Z Cotinine detection and tobacco smoke exposure Cotinine Environmental tobacco smoke Epidemiological studies Second hand smoker Nicotiana tabacum Tobacco is one of the major preventable causes of morbidity and premature mortality in the world. A simple, rapid method for determination of cotinine, major metabolite of nicotine in urine, is described. The assay involves a fast liquid-liquid extraction with chloroform as a solvent in alkaline environment. The extract was dried at 37°C and the residue was dissolved in 100 μL of standard internal of pyridine in methanol solution, and an aliquot was analyzed by gas-liquid chromatograph equipped with a flame -ionization detector. This technique was performed to test urine of a sample of smokers and non-smokers who were previously asked about their smoking habit. The limit of detection was 3.1 ng/mL, and using two calibration curves, a linearity range from 3 a 5.000 ng/mL was covered. The median and quartiles (Q) of cotinine in smokers were 255.4 ng/mL, 15.9 ng/mL (Q1) - 1050.2 ng/mL (Q3) ng/mL and in non-smokers 15.8 ng/mL, 4.1 ng/mL (Q1) - 38.4 ng/mL (Q3). Wilcoxon sumrank: z = 4.865 p < 0.01. There was a relationship between cotinine concentration in urine and number of smoked cigarettes. Spearman's rho = 0.5672 (p < 0.001). This technique could identify smokers, second hand smokers and non-smokers using cut-off of 10-15 ng/mL of cotinine in urine, usually adopted in epidemiological studies. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v40_n2_p181_Vacchino http://hdl.handle.net/20.500.12110/paper_03252957_v40_n2_p181_Vacchino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cotinine Environmental tobacco smoke Epidemiological studies Second hand smoker Nicotiana tabacum |
| spellingShingle |
Cotinine Environmental tobacco smoke Epidemiological studies Second hand smoker Nicotiana tabacum Cotinine detection and tobacco smoke exposure |
| topic_facet |
Cotinine Environmental tobacco smoke Epidemiological studies Second hand smoker Nicotiana tabacum |
| description |
Tobacco is one of the major preventable causes of morbidity and premature mortality in the world. A simple, rapid method for determination of cotinine, major metabolite of nicotine in urine, is described. The assay involves a fast liquid-liquid extraction with chloroform as a solvent in alkaline environment. The extract was dried at 37°C and the residue was dissolved in 100 μL of standard internal of pyridine in methanol solution, and an aliquot was analyzed by gas-liquid chromatograph equipped with a flame -ionization detector. This technique was performed to test urine of a sample of smokers and non-smokers who were previously asked about their smoking habit. The limit of detection was 3.1 ng/mL, and using two calibration curves, a linearity range from 3 a 5.000 ng/mL was covered. The median and quartiles (Q) of cotinine in smokers were 255.4 ng/mL, 15.9 ng/mL (Q1) - 1050.2 ng/mL (Q3) ng/mL and in non-smokers 15.8 ng/mL, 4.1 ng/mL (Q1) - 38.4 ng/mL (Q3). Wilcoxon sumrank: z = 4.865 p < 0.01. There was a relationship between cotinine concentration in urine and number of smoked cigarettes. Spearman's rho = 0.5672 (p < 0.001). This technique could identify smokers, second hand smokers and non-smokers using cut-off of 10-15 ng/mL of cotinine in urine, usually adopted in epidemiological studies. |
| title |
Cotinine detection and tobacco smoke exposure |
| title_short |
Cotinine detection and tobacco smoke exposure |
| title_full |
Cotinine detection and tobacco smoke exposure |
| title_fullStr |
Cotinine detection and tobacco smoke exposure |
| title_full_unstemmed |
Cotinine detection and tobacco smoke exposure |
| title_sort |
cotinine detection and tobacco smoke exposure |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v40_n2_p181_Vacchino http://hdl.handle.net/20.500.12110/paper_03252957_v40_n2_p181_Vacchino |
| _version_ |
1768543181611204608 |