In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis
The chick embryo has been used as a screening system to study porphyrin metabolism, and the toxicological and teratogenic effects of many chemical substances. The aim of this study is to evaluate Cd 2+, Pb 2+, and Cu 2+ teratogenicity; and in vivo effects on chick embryo of d-aminolevulinic acid deh...
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2004
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paper:paper_03252787_v85_n3_p154_Pauza2023-06-08T15:32:09Z In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis Pauza, Noemí Laura Ferramola, Ana María Sancovich, Horacio Alberto Chick embryo D-aminolevulinic acid dehydratase Haem Metals Porphobilinogen deaminase biological marker cadmium cadmium acetate copper copper sulfate heme lead lead acetate liver enzyme membrane enzyme metal porphobilinogen deaminase porphobilinogen synthase animal experiment animal tissue article chick embryo enzyme activity enzyme assay heme synthesis liver mineral metabolism mortality nonhuman teratogenicity vitelline membrane The chick embryo has been used as a screening system to study porphyrin metabolism, and the toxicological and teratogenic effects of many chemical substances. The aim of this study is to evaluate Cd 2+, Pb 2+, and Cu 2+ teratogenicity; and in vivo effects on chick embryo of d-aminolevulinic acid dehydratase (ALA-D) and porphobilinogen deaminase (PBG-D) activities. On day 12 of incubation, White Leghorn eggs were injected in the yolk with cadmium acetate, lead acetate, or copper sulfate in two different doses. The yolk sac membrane and liver were removed 12 and 60 hours later and ALA-D and PBG-D activities assayed. The in vivo Cd 2+ administration in chick embryo induced higher mortality rate and teratogenic effects than Pb 2+ and Cu 2+. The different effects observed on the enzyme activities can be better explained by both (a) differences in metals' capacity to cross the vitelline membrane, and (b) a secondary effect of alterations in general mineral metabolism, more than as a result of direct action on the enzymes themselves. Our results are the first to show that yolk sac membrane enzymes are more sensible than liver enzymes to the action of the metals used in our studies. These findings indicate that the yolk sac membrane is the preferential tissue to be used for metal biomarker toxicity assays. Fil:Pauza, N.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ferramola De Sancovich, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sancovich, H.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252787_v85_n3_p154_Pauza http://hdl.handle.net/20.500.12110/paper_03252787_v85_n3_p154_Pauza |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chick embryo D-aminolevulinic acid dehydratase Haem Metals Porphobilinogen deaminase biological marker cadmium cadmium acetate copper copper sulfate heme lead lead acetate liver enzyme membrane enzyme metal porphobilinogen deaminase porphobilinogen synthase animal experiment animal tissue article chick embryo enzyme activity enzyme assay heme synthesis liver mineral metabolism mortality nonhuman teratogenicity vitelline membrane |
| spellingShingle |
Chick embryo D-aminolevulinic acid dehydratase Haem Metals Porphobilinogen deaminase biological marker cadmium cadmium acetate copper copper sulfate heme lead lead acetate liver enzyme membrane enzyme metal porphobilinogen deaminase porphobilinogen synthase animal experiment animal tissue article chick embryo enzyme activity enzyme assay heme synthesis liver mineral metabolism mortality nonhuman teratogenicity vitelline membrane Pauza, Noemí Laura Ferramola, Ana María Sancovich, Horacio Alberto In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis |
| topic_facet |
Chick embryo D-aminolevulinic acid dehydratase Haem Metals Porphobilinogen deaminase biological marker cadmium cadmium acetate copper copper sulfate heme lead lead acetate liver enzyme membrane enzyme metal porphobilinogen deaminase porphobilinogen synthase animal experiment animal tissue article chick embryo enzyme activity enzyme assay heme synthesis liver mineral metabolism mortality nonhuman teratogenicity vitelline membrane |
| description |
The chick embryo has been used as a screening system to study porphyrin metabolism, and the toxicological and teratogenic effects of many chemical substances. The aim of this study is to evaluate Cd 2+, Pb 2+, and Cu 2+ teratogenicity; and in vivo effects on chick embryo of d-aminolevulinic acid dehydratase (ALA-D) and porphobilinogen deaminase (PBG-D) activities. On day 12 of incubation, White Leghorn eggs were injected in the yolk with cadmium acetate, lead acetate, or copper sulfate in two different doses. The yolk sac membrane and liver were removed 12 and 60 hours later and ALA-D and PBG-D activities assayed. The in vivo Cd 2+ administration in chick embryo induced higher mortality rate and teratogenic effects than Pb 2+ and Cu 2+. The different effects observed on the enzyme activities can be better explained by both (a) differences in metals' capacity to cross the vitelline membrane, and (b) a secondary effect of alterations in general mineral metabolism, more than as a result of direct action on the enzymes themselves. Our results are the first to show that yolk sac membrane enzymes are more sensible than liver enzymes to the action of the metals used in our studies. These findings indicate that the yolk sac membrane is the preferential tissue to be used for metal biomarker toxicity assays. |
| author |
Pauza, Noemí Laura Ferramola, Ana María Sancovich, Horacio Alberto |
| author_facet |
Pauza, Noemí Laura Ferramola, Ana María Sancovich, Horacio Alberto |
| author_sort |
Pauza, Noemí Laura |
| title |
In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis |
| title_short |
In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis |
| title_full |
In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis |
| title_fullStr |
In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis |
| title_full_unstemmed |
In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis |
| title_sort |
in vivo studies of pb 2+, cd 2+ and cu 2+ toxicity and their effects on haem biosynthesis |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252787_v85_n3_p154_Pauza http://hdl.handle.net/20.500.12110/paper_03252787_v85_n3_p154_Pauza |
| work_keys_str_mv |
AT pauzanoemilaura invivostudiesofpb2cd2andcu2toxicityandtheireffectsonhaembiosynthesis AT ferramolaanamaria invivostudiesofpb2cd2andcu2toxicityandtheireffectsonhaembiosynthesis AT sancovichhoracioalberto invivostudiesofpb2cd2andcu2toxicityandtheireffectsonhaembiosynthesis |
| _version_ |
1768546676270694400 |