cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii

Intracellular levels of cAMP and specific activities of adenylate cyclase and cAMP phosphodiesterase were measured during yeast-to-hyphae transition in the dimorphic fungus M. rouxii. Enzymatic activities were measured in permeabilized cells under conditions preventing protein dephosphorylation and...

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Autores principales: Cantore de Ezcurra, María Leonor, Galvagno, Miguel Angel
Publicado: 1983
Materias:
3',5' cyclic nucleotide phosphodiesterase
adenylate cyclase
bucladesine
cyclic AMP
article
comparative study
drug effect
kinetics
metabolism
Mucor
physiology
species difference
3',5'-Cyclic-Nucleotide Phosphodiesterase
Adenylate Cyclase
Bucladesine
Comparative Study
Cyclic AMP
Kinetics
Species Specificity
Support, Non-U.S. Gov't
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v7_n11_p947_Cantore
http://hdl.handle.net/20.500.12110/paper_03091651_v7_n11_p947_Cantore
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03091651_v7_n11_p947_Cantore
record_format dspace
spelling paper:paper_03091651_v7_n11_p947_Cantore2023-06-08T15:31:49Z cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii Cantore de Ezcurra, María Leonor Galvagno, Miguel Angel 3',5' cyclic nucleotide phosphodiesterase adenylate cyclase bucladesine cyclic AMP article comparative study drug effect kinetics metabolism Mucor physiology species difference 3',5'-Cyclic-Nucleotide Phosphodiesterase Adenylate Cyclase Bucladesine Comparative Study Cyclic AMP Kinetics Mucor Species Specificity Support, Non-U.S. Gov't Intracellular levels of cAMP and specific activities of adenylate cyclase and cAMP phosphodiesterase were measured during yeast-to-hyphae transition in the dimorphic fungus M. rouxii. Enzymatic activities were measured in permeabilized cells under conditions preventing protein dephosphorylation and proteolysis. A two-fold decrease in intracellular cAMP levels occurred shortly after exposure of the yeast to air quite before morphological changes became evident. Morphogenesis to hyphae after exposure to air was inhibited by the addition of 10 mM dibutyryl cAMP to the culture medium, and the yeast morphology was maintained for at least 24 hours. The decrease in cAMP levels that occurs shortly after exposure of yeast culture to air was mainly accounted for by variations in the state of activation of cAMP phosphodiesterase while the specific activity of adenylate cyclase did not vary significantly during yeast-to-hyphae transition. © 1983. Fil:Cantore, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Galvagno, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1983 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v7_n11_p947_Cantore http://hdl.handle.net/20.500.12110/paper_03091651_v7_n11_p947_Cantore
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 3',5' cyclic nucleotide phosphodiesterase
adenylate cyclase
bucladesine
cyclic AMP
article
comparative study
drug effect
kinetics
metabolism
Mucor
physiology
species difference
3',5'-Cyclic-Nucleotide Phosphodiesterase
Adenylate Cyclase
Bucladesine
Comparative Study
Cyclic AMP
Kinetics
Mucor
Species Specificity
Support, Non-U.S. Gov't
spellingShingle 3',5' cyclic nucleotide phosphodiesterase
adenylate cyclase
bucladesine
cyclic AMP
article
comparative study
drug effect
kinetics
metabolism
Mucor
physiology
species difference
3',5'-Cyclic-Nucleotide Phosphodiesterase
Adenylate Cyclase
Bucladesine
Comparative Study
Cyclic AMP
Kinetics
Mucor
Species Specificity
Support, Non-U.S. Gov't
Cantore de Ezcurra, María Leonor
Galvagno, Miguel Angel
cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii
topic_facet 3',5' cyclic nucleotide phosphodiesterase
adenylate cyclase
bucladesine
cyclic AMP
article
comparative study
drug effect
kinetics
metabolism
Mucor
physiology
species difference
3',5'-Cyclic-Nucleotide Phosphodiesterase
Adenylate Cyclase
Bucladesine
Comparative Study
Cyclic AMP
Kinetics
Mucor
Species Specificity
Support, Non-U.S. Gov't
description Intracellular levels of cAMP and specific activities of adenylate cyclase and cAMP phosphodiesterase were measured during yeast-to-hyphae transition in the dimorphic fungus M. rouxii. Enzymatic activities were measured in permeabilized cells under conditions preventing protein dephosphorylation and proteolysis. A two-fold decrease in intracellular cAMP levels occurred shortly after exposure of the yeast to air quite before morphological changes became evident. Morphogenesis to hyphae after exposure to air was inhibited by the addition of 10 mM dibutyryl cAMP to the culture medium, and the yeast morphology was maintained for at least 24 hours. The decrease in cAMP levels that occurs shortly after exposure of yeast culture to air was mainly accounted for by variations in the state of activation of cAMP phosphodiesterase while the specific activity of adenylate cyclase did not vary significantly during yeast-to-hyphae transition. © 1983.
author Cantore de Ezcurra, María Leonor
Galvagno, Miguel Angel
author_facet Cantore de Ezcurra, María Leonor
Galvagno, Miguel Angel
author_sort Cantore de Ezcurra, María Leonor
title cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii
title_short cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii
title_full cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii
title_fullStr cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii
title_full_unstemmed cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii
title_sort camp levels and in situ measurement of adenylate cyclase and camp phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus mucor rouxii
publishDate 1983
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v7_n11_p947_Cantore
http://hdl.handle.net/20.500.12110/paper_03091651_v7_n11_p947_Cantore
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