Effects of cholinergic muscarinic agents on protein kinase C activity in rat pineal gland

The role of protein kinase C (PKC) on muscarinic regulation of serotonin release in the pineal gland was investigated by measuring the pineal-PKC activity and serotonin secretion in response to muscarinic agents. Pineal slices, short-term incubated (0-15 min) without additions produced a low seroton...

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Detalles Bibliográficos
Publicado: 1991
Materias:
muscarinic activation
pineal gland
protein kinase C
serotonin
angiotensin
atropine
carbachol
isoprenaline
muscarinic agent
muscarinic receptor
pilocarpine
protein kinase c
staurosporine
animal experiment
animal tissue
article
cholinergic activity
controlled study
enzyme activity
long term exposure
male
metabolic regulation
nonhuman
pineal body
priority journal
rat
serotonin release
Angiotensin II
Animal
Atropine
Carbachol
Cell Membrane
Cytoplasm
Isoproterenol
Male
Parasympathomimetics
Pilocarpine
Pineal Gland
Protein Kinase C
Rats
Rats, Inbred Strains
Serotonin
Support, Non-U.S. Gov't
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v15_n10_p943_Finocchiaro
http://hdl.handle.net/20.500.12110/paper_03091651_v15_n10_p943_Finocchiaro
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_03091651_v15_n10_p943_Finocchiaro
record_format dspace
spelling paper:paper_03091651_v15_n10_p943_Finocchiaro2023-06-08T15:31:48Z Effects of cholinergic muscarinic agents on protein kinase C activity in rat pineal gland muscarinic activation pineal gland protein kinase C serotonin angiotensin atropine carbachol isoprenaline muscarinic agent muscarinic receptor pilocarpine protein kinase c serotonin staurosporine animal experiment animal tissue article cholinergic activity controlled study enzyme activity long term exposure male metabolic regulation nonhuman pineal body priority journal rat serotonin release Angiotensin II Animal Atropine Carbachol Cell Membrane Cytoplasm Isoproterenol Male Parasympathomimetics Pilocarpine Pineal Gland Protein Kinase C Rats Rats, Inbred Strains Serotonin Support, Non-U.S. Gov't The role of protein kinase C (PKC) on muscarinic regulation of serotonin release in the pineal gland was investigated by measuring the pineal-PKC activity and serotonin secretion in response to muscarinic agents. Pineal slices, short-term incubated (0-15 min) without additions produced a low serotonin release and 20 to 24 percent PKC activity was found associated with membrane fractions. Prolonged exposure of pineal slices (30-180 min) produced further translocation of PKC activity to the membranes and a significant increase of serotonin release. Short-term treatment with pilocarpine and carbachol, stimulated PKC activity of both cytosolic and particulate fractions and the release of pineal serotonin. The pilocarpine effect was blocked by atropine indicating that it was mediated by muscarinic receptors. The present data support that PKC activation correlates with the increase of serotonin release by muscarinic agonist in pineal gland. © 1991. 1991 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v15_n10_p943_Finocchiaro http://hdl.handle.net/20.500.12110/paper_03091651_v15_n10_p943_Finocchiaro
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic muscarinic activation
pineal gland
protein kinase C
serotonin
angiotensin
atropine
carbachol
isoprenaline
muscarinic agent
muscarinic receptor
pilocarpine
protein kinase c
serotonin
staurosporine
animal experiment
animal tissue
article
cholinergic activity
controlled study
enzyme activity
long term exposure
male
metabolic regulation
nonhuman
pineal body
priority journal
rat
serotonin release
Angiotensin II
Animal
Atropine
Carbachol
Cell Membrane
Cytoplasm
Isoproterenol
Male
Parasympathomimetics
Pilocarpine
Pineal Gland
Protein Kinase C
Rats
Rats, Inbred Strains
Serotonin
Support, Non-U.S. Gov't
spellingShingle muscarinic activation
pineal gland
protein kinase C
serotonin
angiotensin
atropine
carbachol
isoprenaline
muscarinic agent
muscarinic receptor
pilocarpine
protein kinase c
serotonin
staurosporine
animal experiment
animal tissue
article
cholinergic activity
controlled study
enzyme activity
long term exposure
male
metabolic regulation
nonhuman
pineal body
priority journal
rat
serotonin release
Angiotensin II
Animal
Atropine
Carbachol
Cell Membrane
Cytoplasm
Isoproterenol
Male
Parasympathomimetics
Pilocarpine
Pineal Gland
Protein Kinase C
Rats
Rats, Inbred Strains
Serotonin
Support, Non-U.S. Gov't
Effects of cholinergic muscarinic agents on protein kinase C activity in rat pineal gland
topic_facet muscarinic activation
pineal gland
protein kinase C
serotonin
angiotensin
atropine
carbachol
isoprenaline
muscarinic agent
muscarinic receptor
pilocarpine
protein kinase c
serotonin
staurosporine
animal experiment
animal tissue
article
cholinergic activity
controlled study
enzyme activity
long term exposure
male
metabolic regulation
nonhuman
pineal body
priority journal
rat
serotonin release
Angiotensin II
Animal
Atropine
Carbachol
Cell Membrane
Cytoplasm
Isoproterenol
Male
Parasympathomimetics
Pilocarpine
Pineal Gland
Protein Kinase C
Rats
Rats, Inbred Strains
Serotonin
Support, Non-U.S. Gov't
description The role of protein kinase C (PKC) on muscarinic regulation of serotonin release in the pineal gland was investigated by measuring the pineal-PKC activity and serotonin secretion in response to muscarinic agents. Pineal slices, short-term incubated (0-15 min) without additions produced a low serotonin release and 20 to 24 percent PKC activity was found associated with membrane fractions. Prolonged exposure of pineal slices (30-180 min) produced further translocation of PKC activity to the membranes and a significant increase of serotonin release. Short-term treatment with pilocarpine and carbachol, stimulated PKC activity of both cytosolic and particulate fractions and the release of pineal serotonin. The pilocarpine effect was blocked by atropine indicating that it was mediated by muscarinic receptors. The present data support that PKC activation correlates with the increase of serotonin release by muscarinic agonist in pineal gland. © 1991.
title Effects of cholinergic muscarinic agents on protein kinase C activity in rat pineal gland
title_short Effects of cholinergic muscarinic agents on protein kinase C activity in rat pineal gland
title_full Effects of cholinergic muscarinic agents on protein kinase C activity in rat pineal gland
title_fullStr Effects of cholinergic muscarinic agents on protein kinase C activity in rat pineal gland
title_full_unstemmed Effects of cholinergic muscarinic agents on protein kinase C activity in rat pineal gland
title_sort effects of cholinergic muscarinic agents on protein kinase c activity in rat pineal gland
publishDate 1991
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v15_n10_p943_Finocchiaro
http://hdl.handle.net/20.500.12110/paper_03091651_v15_n10_p943_Finocchiaro
_version_ 1768546116265050112

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